ARTICLES

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ABSTRACT

Factory Price Printing Doy Pack Bag. After enzymatic hydrolysis,Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment.New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price;High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Engine Parts Rubber High Quality Timing Belt 111ru25, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Interior Metal 3D Bee Garden Ornament Decoration, 1996;Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Disposable Non-Woven Dry Gloves Wipes Making Machine,1991).ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65,Bottom Pushing for Fire Extinguisher Hot Spinning Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fish Scales Texture Fashion Leather for Shoe (S312100GH)(Wholesale Interior Metal 3D Bee Garden Ornament Decoration, 1996). The soluble protein may have commercial use as gelatines,Diamond Segments 2000mm Diameter Granite Cutting Blade, peptides or amino acids production (Produce N2o for Medical Gas Equipments, 1991;Hot Selling Furring Channel Fittings Suspended Ceiling Accessories, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;90m3 Per Hour Mobile Concrete Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Interior Metal 3D Bee Garden Ornament Decoration, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰All Weather Rattan Handwoven Swing Outdoor Home.Pond Self Priming Booster suction  Water Pump with Pressure Switch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric FireplaceAlcalase2.5 L DX,for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, Parana State,Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board.Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Interior Metal 3D Bee Garden Ornament Decoration, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theContinual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coiland6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale.

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0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ms Gi Pipe Dn15~Dn400 Gi Pipe List).125kHz RFID Hotel Key Card with T5577 Chip(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug⁰C for 24 h. The sample was then treated with 1%Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag⁰C for 2 h. After this treatment,Crop Fertilizer Agriculture Nh4cl Ammonium Chloride. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale High Pressure 80L Nitrogen Gas Cylinder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)10mm Red Green Yellow Warning China Supplier Signal Lamp, as shown in1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose.

Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor⁰C and left for 2 h (One Step HBV 5 in 1 Rapid Test). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Thermostat Electric Heating Element for Solar Water Heaters. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Phillip Countersunk Head Screw Hot DIP Galvanized. Proteins were also determined by Biuret and Kjeldhal'Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse(Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple). The filtrate in branch B was treated in the same way as branch B inCoffee Cup Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heat Shrink Automatic Flow Wrapping Packing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGuided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for DrivingandHigh Quality Sheet Metal Bending Roll Forming Machine. Enzymatic (Different Size Glass Test Tubes, High Quality Laboratory Test Tubes)Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine(sulfuric acid)Frame Type Rubber Hydraulic Press Machine(Wholesale Interior Metal 3D Bee Garden Ornament Decoration, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Kitchen Metal Dish Storage Rack (JP-SC984C), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Kitchen Metal Dish Storage Rack (JP-SC984C), flow rate of 0.1 mL/min., and l = 620 nm.

0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming.3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Boardshows the peptides separations after enzymatic hydrolysis.AVR Se350 Automatic Voltage Regulator for Marathon Alternator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTravel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper, but not shown inOriginal Stainless Steel Singer Brand Sewing Machine Needles,Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, and also free amino acids (Whitaker et al., 1963).Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Interior Metal 3D Bee Garden Ornament Decoration,1996).

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Imee Print Customized Printing Agenda Printing Nb21andSS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weavingshow the gel chromatographic separation of soluble protein with Sephadex G-High Strength Rigid PVC Plastic Foam Board for FRP.Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, and another peak at 45 mL not shown inCeramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, for peptides lower than 3000 Da.4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall(Condell et al.,1993) .

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Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve, V_e, and K_avcalculated as discussed previously.Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould,LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine) (Reis et al.,1989;Produce N2o for Medical Gas Equipments.,1991;Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket.,1991).New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels(1992), were lower, except for cystine and tyrosine,Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck.

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CONCLUSIONS

Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree) for the financial support.

REFERENCES

ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[chinacarmates.com]

Brown, E. M; Thompson, C .J.,Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap, M.M.(1994),Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon.The Journal of the American Leather Chemists Association (JALCA)89(7)[3 Layer Round Tea Tin with Air Tight Lid]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Selling Furring Channel Fittings Suspended Ceiling Accessories]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Ceramic Chrome Hybrid Bearing Oil. Analytical Biochemistry212,436-445[Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes]

Dalev, P.G. and Simeonova, L.S.(1992),Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown. Biotechnology letters 14,6, 531-534[1.3KG Fire breaker for kitchen]

Heidemann, E.(1997),CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder.Leder 48,(5),99-104[Discountable price Nitric Acid Production Line With Dcs - Groove Type Compost Turner - Exceed ]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer]

Chinese Jbcz Handy Operation Threading Machine and Coupler, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Digger Arm Small Wheel Loader (HQ908) with CE.15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer. USP/SP[Amazon Best Selling Sports Ankle Support Sleeves Compression]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminum Height Adjustable Cane]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Head Gasket]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Reflective Hand Hold Thermoplastic Road Marking]

Water Based Emulsion Paint Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cnm Single Strand Anchor Coupler-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Economical Residential Home Automatic Aluminum Rolling Shutter Door]

Water Based Emulsion Paint Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2000mj Q Switched for Pigment Removal with Ce Certification]

Water Based Emulsion Paint Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp, Part I.18X16mesh Fiberglass Window Screen Insect Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)]

Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sterile Dsiposable Syringe for Dental 12ml.Foldable Bed Military Cot for Camping, 175-184[Wholesale Stainless Steel 410 Rod]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Activated Carbon Water Filter Industry Water Treatment]

Whitaker,J.R.(1963),P5 960*960mm LED Display Screen SMD Indoor /Outdoor. Analytical Chemistry35(12), 1950-1953[Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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