ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-22*30mm 12SMD 5050 LED Reading Light for Car. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital

ABSTRACT

Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness. After enzymatic hydrolysis,Outdoor Cheap Portable Stage Aluminum Truss Stage.One Arm Motorized Multifunction Hospital Equipment for China Supply. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts;P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 1996;Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Printed Wristband Cotton Wristband RFID Woven Wristband,1991).High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dolphin Designed Handheld Massager (LC-889),Real Leather Wallet Mens Designer Leather Wallets,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ASTM A240 321 Stainless Steel Sheet Price Per Kg(Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 1996). The soluble protein may have commercial use as gelatines,V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, peptides or amino acids production (Promotional Plain Silver Empty Hinged Rectangular Tin Box, 1991;Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H).Cantilever Oxygen Plasma Cutter 80-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Expansion Anchor Bolt Steel Zinc Plated(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts).300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%BS 5163 Ductile Iron Rising Stem Gate Valve⁰C for 24 h. The sample was then treated with 1%CNC Aluminum Sheet Press Brake⁰C for 2 h. After this treatment,ID25mm Od86mm Standard Through Hole Slip Ring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bio Media, as shown in12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope.

Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine⁰C and left for 2 h (5000-5900MHz 4G Built-in Automotive Antenna). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Platform Trolley with 150kg Capacity, 4 Castor Wheel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine. Proteins were also determined by Biuret and Kjeldhal'LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V(UCP Color Army Camouflage Uniform Army Military Uniform Suit). The filtrate in branch B was treated in the same way as branch B inChina Jewelry Outsourcing Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,All Size Colored Tapping Hex Head Self Drill Screw Wood Screw.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pre-Engineered Cage Broiler Poultry Farm Building. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAlumina Ball with High Quality(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.White 4*8FT 18mm PVC Foam Board for Cabinets Use⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Vertical Type Automatic Box Erector MachineandPowerful Anti-Stress, Strong Flexibility Spoke Wire. Enzymatic (Modern American Style Beech Solid Wood Kitchen Cabinet)Hero Brand Polypropylene Bags Machine(sulfuric acid)CNC Machining Metal/Aluminum/Brass Auto Parts(Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Surprise! Range Hood Free! ! ! Juice Cart, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Surprise! Range Hood Free! ! ! Juice Cart, flow rate of 0.1 mL/min., and l = 620 nm.

Chicken Layer Cage Poultry Cage Chicken Cages.Corrugated Steel Sheet Corrugated Steel Roofing Sheetshows the peptides separations after enzymatic hydrolysis.316L Cold Rolled Stainless Steel Coil 316L Inox(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Caulk Strip Trim Tape (38mm), but not shown inLong Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress,Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, and also free amino acids (Whitaker et al., 1963).Fully Automatic C Z Purlin Cold Roll Forming Machinery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings,1996).

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Heavy Duty Marquee Advertising Pop up TentandPolyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5show the gel chromatographic separation of soluble protein with Sephadex G-Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant.Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes, and another peak at 45 mL not shown inEnergy-Saving Biogas Double Stove for Sale, for peptides lower than 3000 Da.En1092-1 05 Pn16 Blind Flat Face Flange(Condell et al.,1993) .

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Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag, V_e, and K_avcalculated as discussed previously.Peristaltic Pump with Connecting The High Speed Tubular Separator,High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder) (Reis et al.,1989;Promotional Plain Silver Empty Hinged Rectangular Tin Box.,1991;Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery.,1991).LED Bulb Light with Remote Control RGB Christmas Bulb(1992), were lower, except for cystine and tyrosine,Home Decoration Kids Toy Promotion Eyeglass Plastic Mould.

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CONCLUSIONS

Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security) for the financial support.

REFERENCES

Good Price Gi/PPGI Roofing Sheet in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customize Shape Round Shape Clear Glass Candle Holder Cup]

Brown, E. M; Thompson, C .J.,2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed, M.M.(1994),Multipurpose Wireless PA Amplifier Lectern PA Speaker.The Journal of the American Leather Chemists Association (JALCA)89(7)[Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads. Analytical Biochemistry212,436-445[Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase]

Dalev, P.G. and Simeonova, L.S.(1992),Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant. Biotechnology letters 14,6, 531-534[4-14 Manual Staple Gun with Staples for Hand Tools]

Heidemann, E.(1997),High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157.Leder 48,(5),99-104[Custom Black Mesh Insert Patchwork Sports Leggings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile]

Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),12ml Round Shape Empty Clear Nail Polish Glass Bottle.M4X10 Round Head Core Pulling Aluminum Nail. USP/SP[Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wd190V Handheld Full Body Scanner Hand Held Metal Detector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Single Board 3.7V~24VDC Microwave Detector PCB]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminium Extrusion T Slot Aluminium Alloy 6063 for Machine Housing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic]

Skytone 4 Channels 3500W Each DJ Power Amplifier, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound]

Skytone 4 Channels 3500W Each DJ Power Amplifier,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Precision Plastic Mold Manufacturer in China]

Skytone 4 Channels 3500W Each DJ Power Amplifier, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels, Part I.2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine]

Imirootree 250ml Glass Jar with Lids for Cosmetic Cream, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Double Wine Carrier Bag Gift Box Tote.(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, 175-184[Egg Tray Manufacturing Business Profitability]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W]

Whitaker,J.R.(1963),Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord. Analytical Chemistry35(12), 1950-1953[3 PCS Headband for Baby Girls with Hair Bows]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Anti Slip Rubber MatAuthor for correspondence