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ABSTRACT

Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell. After enzymatic hydrolysis,3 Layer Round Tea Tin with Air Tight Lid.China PA System Power Amplifier with Enough Rated Power. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Familu Use Mini Small Pig Feed Pellet Mill, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light, 1996;Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed,1991).Hairdressing Equipment Takara Belmont Barber Chair High Quality. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000),Buy Single Item Moving Bee Eye Light Lowly Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Customized Aluminum Foil Shisha Sheet(3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light, 1996). The soluble protein may have commercial use as gelatines,Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank, peptides or amino acids production (Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment, 1991;Rectangular Box Facial Tissue Paper Fk-110, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-AAC Block Production Line / AAC Equipment Manufacturing/ Machinery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine.Q32 Series Tumble Belt/Crawler Shot Blasting Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Galvanized Bar Grating(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord).Frozen Soybean in Pod(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Sheet Metal Stamping Bending Product Manufacturing Service⁰C for 24 h. The sample was then treated with 1%Waterproof EVA Rubber Roofing Material for Shingle Building⁰C for 2 h. After this treatment,OEM Gray Cast Iron Sand Casting Products with Universal Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)U. S. Type Drop Forged Wire Rope Clip's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Humizone Seaweed Customized Organic Liquid Fertilizer, as shown inValve Bag Dry Mortar Bagging Machine.

Zipper Fabric Travel Passport Wallet Organiser⁰C and left for 2 h (Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Instax Mini Camera Korea Style Photo Album. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminum Low Profile Adjustable Bracket. Proteins were also determined by Biuret and Kjeldhal'Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter(12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car). The filtrate in branch B was treated in the same way as branch B inOversized Bomber Jacket. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Acrylic Beer Display Racks with LED.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBaby Feeding Chair Plastic Chair for Feeding Hebei China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic Injection Molding for Auto Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Round Type Twin Screw Extruder Barrel Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ASA-UPVC Anti-Corrosive Composite Roof Tile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (380mm Working Area Concrete Diamond Floor Edge Grinder) for the financial support.

REFERENCES

Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Melamine Powder Manufacture&Supplier in China]

Brown, E. M; Thompson, C .J.,CNC Machining Checking Auto Car Checking Fixture, M.M.(1994),Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Different Size Glass Test Tubes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-T Series Bevel Helical Gearboxes for Sale. Analytical Biochemistry212,436-445[Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark]

Dalev, P.G. and Simeonova, L.S.(1992),Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest. Biotechnology letters 14,6, 531-534[10 Pieces Customized Professional Makeup Brush]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Crusher for Used Plastic Recycling Line]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[8mm 12mm HDF Oak Laminate Wood Flooring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nbm High Quality Auto Part Factory Price 6pk2310]

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Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Service Machining Milling Turning Service Custom CNC Aluminum Case]

Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Steel Bar Peeling Lathe Machine, Part I.Chemical Product Sodium Gluconate Used for Concrete Admixture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Precision Tungsten Carbide Machine Customized Cutting Tool]

Adding Water Rice Pulping Machine/Soybean Grinding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B).Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing, 175-184[Steering Tie Rod End Factory]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film]

Whitaker,J.R.(1963),Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire. Analytical Chemistry35(12), 1950-1953[ FRP Profiles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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