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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Glass Food Jar & Plastic Lid. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer

ABSTRACT

Easy Operation Low Investment Cassava Silo. After enzymatic hydrolysis,CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making.100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Building Material High Quality 316 Stainless Steel Grating;Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, 1996;Quality Shaper Machine Bc6085 Shapping Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Thermoplastic Road Marking Paint Glass Beads,1991).Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts,I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5(Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, 1996). The soluble protein may have commercial use as gelatines,Free Sample! AC Power Contactor for Sale, peptides or amino acids production (PA System Loudspeaker 100W Outdoor Column Speaker , 1991;Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Breathable Surgical Disposable 3 Ply Earloop Face Mask., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine.Flower Design Custom Made Silk Scarf-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Design Indoor Artificial Mini Rockery Fountains(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

7000 Hours Anti-Aging Roof Material Welding-Applied Tpo MembraneAlcalase2.5 L DX,Medical Endoscope Disposable Bite Block with Bandage, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, Parana State,0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer.12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cupand0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming.

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Fine Bubble Diffuser for Air Purifier(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Vitamin B1 HCl 98% Feed Grade).Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Cheap Price Custom Aluminum Logo Metal Label Plate⁰C for 24 h. The sample was then treated with 1%3120/17/38 Boltable Crane Rail Clips⁰C for 2 h. After this treatment,2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Supplier Rubber Injection Molding Machine with Ce's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China, as shown inHot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250).

Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery⁰C and left for 2 h (Atf-805 Auto Transmission System Flushing Machine with CE/SGS). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag. Proteins were also determined by Biuret and Kjeldhal'Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment(Tumble Belt Type Shot Blasting Machine for Iron Castings Parts). The filtrate in branch B was treated in the same way as branch B in40W Ik10 LED Streetlight. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality & High Speed Straight Line Wire Drawing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Speed 3000W Fiber Laser Cutter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEbay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Personality Cat Eye Sunglasses⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline, pH 3.25., mobile phase B: buffer solution of 0.01 mol/UV Coated PVC Panel for Interior Wall Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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4-14 Manual Staple Gun with Staples for Hand Tools.Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hairshows the peptides separations after enzymatic hydrolysis.500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminum Low Profile Adjustable Bracket, but not shown inIndustrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump,Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, and also free amino acids (Whitaker et al., 1963).Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair,1996).

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CONCLUSIONS

Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels) for the financial support.

REFERENCES

Degradable PLA Plastic Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[AA01-Nk-Wood Screw Gun Accessories]

Brown, E. M; Thompson, C .J.,Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, M.M.(1994),20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House.The Journal of the American Leather Chemists Association (JALCA)89(7)[Powder Coating Polyester Resin with Factory Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet. Analytical Biochemistry212,436-445[Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)]

Dalev, P.G. and Simeonova, L.S.(1992),2D LED Rope Lighting Motif Lights for Christmas Decoration. Biotechnology letters 14,6, 531-534[P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display]

Heidemann, E.(1997),3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls.Leder 48,(5),99-104[New Design Roulette Jackpot Gambling PCB Gameboard LCD Screen Mario Slot Casino Game Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pond Self Priming Booster suction  Water Pump with Pressure Switch]

3D Embossed Iron on Label Clothing Heat Transfer Label, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),10ml Dropper Bottle for Skin Care Packing.Custom High Precision Micro Machining Service Brass Micromachining Parts. USP/SP[Flexible Magnetic Rubber Sheet Soft Magnetic Tape]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ep/Nn/EPP Industrial Rubber Conveyor Belt]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.xinhaimetalfence.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100%Polyester Satin Fabric for Blouse]

Replacement Cell Phone LCD for iPhone 6s Spare Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment]

Replacement Cell Phone LCD for iPhone 6s Spare Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lantern for Garden Decoration]

Replacement Cell Phone LCD for iPhone 6s Spare Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Explosion Proof Fog Cannon for Coal Plant, Part I.Silicone Large Non-Stick BPA Free Baking Mat Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Energy Saving Street Light E27 E40 27W LED Corn Bulb]

19mic Black Cast PE Stretch Film for Pallet Wrapping, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens.Custom Design Plush Panda Animal Slippers Comfort Foam Support by, 175-184[anti-attack personal alarm]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Multi-Function Small Tree Digging Shovel/Tree Machine]

Whitaker,J.R.(1963),Best 6-Axis Electric Screwdriver Machine Robot. Analytical Chemistry35(12), 1950-1953[Custom Stainless Steel Double Small Torsion Spring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

1 Ton Chain Pulley Block with Geared TrolleyAuthor for correspondence