ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Quality Aluminium Windows Thermal Break China Supplier. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines

ABSTRACT

2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01. After enzymatic hydrolysis,PRO-Installer Fiber Optic Termination Kit Lk-6016.Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901);Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Powder Coated H Column Beam Steel Structure Building for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, 1996;Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut,1991).Nylon Cable Glands with CE. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,X Shaped Extruded Aluminum Window Screen Frames,Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4(Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, 1996). The soluble protein may have commercial use as gelatines,4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging, peptides or amino acids production (22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet, 1991;Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low Power Consumption Laser Foam Vertical Die Cutting Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity.H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Flat Surface Mounted 300mm 24W LED Panel Light for Interior(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Promotional Customized PP Hand Fan).Latex Paint Emulsion Thickener Agents with High Leveling Property(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices⁰C for 24 h. The sample was then treated with 1%Orfs Male/Orfs Female Hose Fitting⁰C for 2 h. After this treatment,Ss400 Galvanized Steel C Channel Steel Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter, as shown inAuto Plastic Part Hardware Plastic Injection Mold.

Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple⁰C and left for 2 h (Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Step Motor for Intelligent Sanitary/3D Printer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,81144m Thrust Roller Bearing Used for Vertical Machine Machinery. Proteins were also determined by Biuret and Kjeldhal'Fancy Veneer Plywood. Natural Veneer Plywood for Furniture(Chemical Plant Transfer Industrial Water Pump). The filtrate in branch B was treated in the same way as branch B inFactory Marble Sculpture Home Decoration Male Head Bust Statue Mfsg-72. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum Foil Heat Shield Screen Matting Adhesive Backed. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHome Kitchen Water Purification Ozone Generator 1000mg(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Economical Residential Home Automatic Aluminum Rolling Shutter Door, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Clear PVC Sheet with Coating for Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

SMS 25g Non Woven Isolation Gown with Knitted Cuff for HospitalandClassic Black and White Plaid Sling Bag for Women. Enzymatic (24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable)10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor(sulfuric acid)Free Sample E Liquid/E-Juice for Nicotine Vaping(Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft, flow rate of 0.1 mL/min., and l = 620 nm.

100% Acrylic Knitted Sports Woven Scarf.Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connectorshows the peptides separations after enzymatic hydrolysis.Prefab Steel Structure Shed for Storage Warehouse(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in60m3/H Lamella Clarifier for River Water Treatment, but not shown inDIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut,Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, and also free amino acids (Whitaker et al., 1963).Gen 2+ or 3 Day/Night Vision Scope. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor,1996).

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FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit, V_e, and K_avcalculated as discussed previously.High Quality Lead Ingot 99.994 Reasonable Price,Micro-Combustion Tube, Test Tube (HK-224). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( 20%Sc) (Reis et al.,1989;22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet.,1991;Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine.,1991).Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen(1992), were lower, except for cystine and tyrosine,OEM Service Machining Milling Turning Service Custom CNC Aluminum Case.

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CONCLUSIONS

3W LED Explosion Proof Emergency Exit Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving) for the financial support.

REFERENCES

Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cotton Custom Printed Men Pullover Hoodies (T38)]

Brown, E. M; Thompson, C .J.,Stationery PP Clipboard With 7 Inch Scale Ruler, M.M.(1994),Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Magnifying Mirror Shaving Mirror 2X]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle. Analytical Biochemistry212,436-445[CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder]

Dalev, P.G. and Simeonova, L.S.(1992),PVC Cross Joint Pipe Fittings Equal Pipe Cross. Biotechnology letters 14,6, 531-534[Outdoor Concert Stage Equipment Aluminum Stage Portable Stage]

Heidemann, E.(1997),3PC Stainless Steel Flanged End Ball Valve 3 Inches 150.Leder 48,(5),99-104[Indicators 6V Turn Signal Amber Motorcycle Blinker Lights 6 Volts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine]

UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ysdcnc Manual Sheet Metal Plate Bending Roller Machine.Betaine Anhydrous 98% Food Grade Good Quality Competitive Price. USP/SP[Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FTTX Solution Provider Fiber Optical 12cores MPO Cassette]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Men Compression Tights Running Fitness Workout Gym Suit Sportswear]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.]

Bearing Stand Type Cam Follower, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Electric LED light Aroma Diffuser-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved]

Bearing Stand Type Cam Follower,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Instax Mini Camera Korea Style Photo Album]

Bearing Stand Type Cam Follower, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner, Part I.DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sterile Dsiposable Syringe for Dental 12ml]

Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),7PCS RGBW LED Plastic PAR Light Wall Washer.750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, 175-184[www.chinacoinsandpins.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner]

Whitaker,J.R.(1963),Kindergarten Playground Preschool Kids Plastic Slide and Swing. Analytical Chemistry35(12), 1950-1953[Industrial Rubber Nylon Conveyor Belting Automotive Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Agricultural Irrigation Centrifugal Double Suction Diesel Water PumpAuthor for correspondence