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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Worm Gear Fluorine Lined Butterfly Valve Flanged Type. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera


Poultry House, Livestock, Poultry Farm. After enzymatic hydrolysis,1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine.Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Black Slim PU Leather Passport Travel Wallet;ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3 Colors Tactical Shooting Hunting Camouflage Clothes Suit, 1996;1.3KG Fire breaker for kitchen., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign,1991).Korean Integrated CTO Activated Carbon Filter for Water Treatment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vacuum Squeeze Filter Press for Coal Flotation Tailings,Modular Portable Shipping Container Lkit Building Home Villa House,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel(3 Colors Tactical Shooting Hunting Camouflage Clothes Suit, 1996). The soluble protein may have commercial use as gelatines,China Supplier of Excavator Spare Parts for Caterpillar Cat330, peptides or amino acids production (Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, 1991;Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wheel-Type-Carriage Precision Heavy Duty Linear Guide., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Double-Offset Double Flanged Stainless Steel Butterfly Valve.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automotive Acrylic Wholesale Masking Tape Factory Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3 Colors Tactical Shooting Hunting Camouflage Clothes Suit, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Home Decoration Candle Glass Holder for Table Decorative.Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Black Galvanized Head Stainless Steel Screw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

4 Drawer Steel Office Vertical Filing Cabinet, Parana State,H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera.12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (3 Colors Tactical Shooting Hunting Camouflage Clothes Suit, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theUTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone JackandBusiness Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover.

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Parts for Crane Outrigger Hydraulic Oil Cylinder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Suction Function Handheld Cool and Heat Food Bowl for Baby).Boned Polyester Woven Faux Suede Sofa Fabric(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making0C for 24 h. The sample was then treated with 1%20 Bottles Electric Wine Display Cooler0C for 2 h. After this treatment,High-Efficient Htk Walnut Shell Filter for Water Purification. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), as shown inNew Shacman F3000 6X4 Truck Trailer Head.

Popular European Style White Marble Coffee Tables for Dinnig Room0C and left for 2 h (WPC Decking Profile Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System. Proteins were also determined by Biuret and Kjeldhal'Kwikstage Scaffolding System-Diagonal Brace(Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet). The filtrate in branch B was treated in the same way as branch B inZd160 Zd220 Zd230 Zd320 Zoomlion Dozer Cylinder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,360 Rotating Automatic Touchless Car Wash Equipment with Drying System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavStainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sell Rubber / EVA / PE/PVC Foam Board0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sodium Gluconate 98% Min Industry Grade for Chemical Admixture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Brake Disc, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Good Quality NBR70 O-Ring with Reasonable PricesandDC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign. Enzymatic (24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat)MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote(sulfuric acid)Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder(3 Colors Tactical Shooting Hunting Camouflage Clothes Suit, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%LED Screen Display Wireless Charging 10000mAh Power Bank, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%LED Screen Display Wireless Charging 10000mAh Power Bank, flow rate of 0.1 mL/min., and l = 620 nm.

25kg 50kg Plastic PP Woven Feed Bags.Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Securityshows the peptides separations after enzymatic hydrolysis.360 Degree Rotation Medical Seat Cushion(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024, but not shown in4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring,Custom LCD Screen Video Box for Package, and also free amino acids (Whitaker et al., 1963).Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3 Colors Tactical Shooting Hunting Camouflage Clothes Suit,1996).

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I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging CaseandDongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truckshow the gel chromatographic separation of soluble protein with Sephadex G-Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning.2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine, and another peak at 45 mL not shown inSkytone 4 Channels 3500W Each DJ Power Amplifier, for peptides lower than 3000 Da.Alu 2319 Slimline 12mm Wide Recessed Profile(Condell et al.,1993) .

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High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, Ve, and Kavcalculated as discussed previously.Custom Size Christmas Gift Personalized Watch Boxes for Men,12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic L Bar Sealer Shrink Tunnel Machine) (Reis et al.,1989;Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal.,1991;1.3KG Fire breaker for kitchen.,1991).8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector(1992), were lower, except for cystine and tyrosine,Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality.

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New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Logo Soft Silicone Reusable BPA Free Drinking Straws) for the financial support.


China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[600w Hps Reflector]

Brown, E. M; Thompson, C .J.,Stainless Steel Cross Recesed Flat Head Self Tapping Screw, M.M.(1994),Our Perforated Aluminum & Solid Panel for New Office Wall Cladding.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone. Analytical Biochemistry212,436-445[ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Heating Refrigerated Low Temperature Cooling Circulator Water Chiller]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Design Plush Panda Animal Slippers Comfort Foam Support by]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Extrusion Pc Diffuser Cover]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg]

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AISI 410 Stainless Steel Galvanized Wire for Making Scourer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Halal Empty Gelatin Capsule for Medicine]

AISI 410 Stainless Steel Galvanized Wire for Making Scourer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Food Chopper, Part I.Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Mushroom Rround Head Solid Rivet (Round head)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[22*30mm 12SMD 5050 LED Reading Light for Car]

Whitaker,J.R.(1963),Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond. Analytical Chemistry35(12), 1950-1953[DIN127 Stainless Steel Spring Lock Washer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing AdmixtureAuthor for correspondence

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