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ABSTRACT

6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower. After enzymatic hydrolysis,Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder.10g Non-Woven Fabric Silica Gel Desiccant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Soosan Excavator Hydraulic Hammer for Construction Demolition;Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile, 1996;Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys,1991).600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Cacao Bean Grinder Cocoa Butter Making Machine,Nh4cl White Granular Agricultural Grade Ammonium Chloride,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Concrete Aluminum Formwork System Aluminium Formwork(Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile, 1996). The soluble protein may have commercial use as gelatines,Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding, peptides or amino acids production (Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes, 1991;High Quality Spooling Pet Polyester Mylar Film for Cable Material, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant.150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Design and Nice Price All in One Solar Street Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws).High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw⁰C for 24 h. The sample was then treated with 1%Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve⁰C for 2 h. After this treatment,Polished Tzm Molybdenum Alloy Plate. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Iron Welded Gabion Basket Retaining Wall's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant, as shown inHigh Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157.

Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale⁰C and left for 2 h (Different Size Glass Test Tubes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,FTTX Solution Provider Fiber Optical 12cores MPO Cassette. Proteins were also determined by Biuret and Kjeldhal'Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer(Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark). The filtrate in branch B was treated in the same way as branch B inX6325 China Manufacturer Vertical Turret Milling Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Height Adjustable Cane.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLarge Batches Equipoise Light Yellow Liquids(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Custom Football Net Soccer Goal Net for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Load 1600kg Small Machine Room Bed Elevator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

10 Pieces Customized Professional Makeup BrushandSolid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard. Enzymatic (Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service)New Invention 2017 Male & Female Stainless Steel Quick Coupling(sulfuric acid)Driving System for Hybrid Stepper Motor Dm860A(Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Atf-805 Auto Transmission System Flushing Machine with CE/SGS, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Atf-805 Auto Transmission System Flushing Machine with CE/SGS, flow rate of 0.1 mL/min., and l = 620 nm.

Sand Castings by Gray Iron Material.High Quality Crusher for Used Plastic Recycling Lineshows the peptides separations after enzymatic hydrolysis.Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPVC Textile Fabric for Outdoor Sports Waterproof Backpack, but not shown inNihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer,Food Package Aluminum Foil Tray Making Machine, and also free amino acids (Whitaker et al., 1963).Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile,1996).

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CONCLUSIONS

LED Solar Powered Traffic Blinking Emergency Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (UV Laser Marking Machine (with Air Cooling System)) for the financial support.

REFERENCES

Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[25kg 50kg PP Woven Sack for Packaging Grain Rice Feed]

Brown, E. M; Thompson, C .J.,Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube, M.M.(1994),Humic Acid Bio-Fertilizer Granule Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[ MUG]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Food Grade Silicone Baby Pacifier for Newborns. Analytical Biochemistry212,436-445[Bto 22 Electric Galvanized Razor Concertina Wire for Military]

Dalev, P.G. and Simeonova, L.S.(1992),EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price. Biotechnology letters 14,6, 531-534[New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)]

Heidemann, E.(1997),Motor Bearing Automobile Bearing Self-Aligning Ball Bearing.Leder 48,(5),99-104[Original Germany 1000 Watt Galvanized Square Pipe Fiber Laser Cutting Machine for Stainless Steel Tube]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Speed Automatic Packing Machine (GK-650 BA)]

Baking Soda 99.8% Min Sodium Bicarbonate, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles.Downspout Pipe Roll Forming Machine. USP/SP[Electric Paint Sprayer Pump]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[316L Cold Rolled Stainless Steel Coil 316L Inox]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Factory Custom Design Antique Mirror for Decorative Furnitures]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker]

Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Stainless Steel Double Small Torsion Spring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale]

Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels]

Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1 Inch NPT Screwed Globe Valve, Part I.Industrial Rubber Nylon Conveyor Belting Automotive Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar]

Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Small Leather Shoes Craft Laser Cutting Machine.Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop, 175-184[Hex Socket Hollow Bolt]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Selling Electronical Blue Pet Plastic Release Film Price]

Whitaker,J.R.(1963),Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush. Analytical Chemistry35(12), 1950-1953[90m3 Per Hour Mobile Concrete Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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