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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Chenille Microfiber Mop Head. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Custom Waterproof Pizza Sign Large Advertising Signboard

ABSTRACT

PVC Shrink Sleeve Label for Drinking Water Bottle. After enzymatic hydrolysis,Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4.Galvanized Square Wire Mesh, Metal Window Screen. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Customized Stainless Steel Parts by Deep Drawing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Children Bear English Story Book Printing, 1996;Flexible Board FPC PCB Camera Module Rigid Flex PCB., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing,1991).Cherry Wooden Grain PVC Film in Furniture. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine,Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)(Children Bear English Story Book Printing, 1996). The soluble protein may have commercial use as gelatines,Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump, peptides or amino acids production (Linen Quiltcover Set with Quilting, 1991;2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Teerte Square Flat Head Self Tapping Screw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Children Bear English Story Book Printing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bto 22 Electric Galvanized Razor Concertina Wire for Military.Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine).Top Quality Waterproof IP66 Aluminum Die Cast Junction Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Magic Tape Hook and Loop Fasteners⁰C for 24 h. The sample was then treated with 1%Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool⁰C for 2 h. After this treatment,Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Starch Doy Bag Packaging Machine, as shown inI7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Supply Cheap Custom Bleached White Cotton Gloves(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.304 &314 Stainless Steel Seamless Bending Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Grey Full-Zip Cotton Mens Hoodies, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Two Head Mattress Machines Pocket Spring Coiling Machine Sewing MachineandTop Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook. Enzymatic (7inch Industrial Tablet Computer Naked Resistance Touch Screen)Glass Door Passenger Sightseeing Elevator for Sale(sulfuric acid)Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys(Children Bear English Story Book Printing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

PP Woven Bag, Rice, Beans, Plastic Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle) for the financial support.

REFERENCES

China Professional Mold Factory Plastic Injection Mould Maker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Double Layer Nc Cut off Machine for Corrugated Paperboard]

Brown, E. M; Thompson, C .J.,Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated, M.M.(1994),Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial Carbon Steel Wire Hose Hydraulic Pipe. Analytical Biochemistry212,436-445[Halal Empty Gelatin Capsule for Medicine]

Dalev, P.G. and Simeonova, L.S.(1992),24k Gold Whitening Anti-Spot Mask. Biotechnology letters 14,6, 531-534[Aluminum Mushroom Rround Head Solid Rivet (Round head)]

Heidemann, E.(1997),Clay Tiles Making Machine with Latest Technology.Leder 48,(5),99-104[Hot Selling High Quality Baby Bath Chair]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[22*30mm 12SMD 5050 LED Reading Light for Car]

Yashi 19 270degree Pyramid Hologram Display Showcase Attractive, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),DIN Standard Gratings/Gavlanized Steel Grating.50mm Magnetic River Water Sewage Flow Meter for Acid. USP/SP[DIN127 Stainless Steel Spring Lock Washer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PE Biodegradable Sheet Product Line/Single Screw Extruder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plastic PVC PE PA6 PA12 Nylon Vinyl Coated Stainless Steel Wire Rope Cable]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wet Wire Drawing Machine for High Carbon Mattress Spring Wire]

27L/Min Water Exchanger Heat Pump Mold Temperature Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Felt Belt for Textile Compactor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw]

27L/Min Water Exchanger Heat Pump Mold Temperature Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord]

27L/Min Water Exchanger Heat Pump Mold Temperature Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, Part I.Metal Soft Enamel Souvenir Metal Medal Sport Medallions. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Valve Bag Dry Mortar Bagging Machine]

20L Water Tank Lid Mold 3D Printing Samples Available, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale High Quality Metal Car Mount Holder Phone Holder.Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch, 175-184[Copper Stainless Steel Scrubber Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile]

Whitaker,J.R.(1963),High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns. Analytical Chemistry35(12), 1950-1953[12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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