Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace

SciELO - Scientific Electronic Library Online

vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
Home Pagealphabetic serial listing

Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo


Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed

Strong Aluminum Armrest Reclining Hydraulic Barber ChairEdivaldo E.GarciaI; Cláudia C.M.Hairdressing Equipment Takara Belmont Barber Chair High QualityI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiFull Color Child Book Printing Service

IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Buy Single Item Moving Bee Eye Light Lowly Price


High Quality Customized Aluminum Foil Shisha Sheet. After enzymatic hydrolysis,Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank.Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rectangular Box Facial Tissue Paper Fk-110;SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, 1996;CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Q32 Series Tumble Belt/Crawler Shot Blasting Machine,1991).Galvanized Bar Grating. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Custom Self Adhesive Leather Patch for Jackets,Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,380mm Working Area Concrete Diamond Floor Edge Grinder(AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, 1996). The soluble protein may have commercial use as gelatines,Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam, peptides or amino acids production (New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate, 1991;Discussion and Interpretation System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Frozen Soybean in Pod.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Sheet Metal Stamping Bending Product Manufacturing Service. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Waterproof EVA Rubber Roofing Material for Shingle Building.OEM Gray Cast Iron Sand Casting Products with Universal Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:U. S. Type Drop Forged Wire Rope Clip(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Humizone Seaweed Customized Organic Liquid FertilizerAlcalase2.5 L DX,Zipper Fabric Travel Passport Wallet Organiser, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Hot Sale Instax Mini Camera Korea Style Photo Album); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Aluminum Low Profile Adjustable Bracket(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter, Parana State,Buy Single Item Moving Bee Eye Light Lowly Price.High Quality Acrylic Beer Display Racks with LED, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the1000base Single Mode 1310nm LC SFP Module 20km 1.25gandChina PA System Power Amplifier with Enough Rated Power.

Sud1000h Poly Welder Butt Fusion Machine

3DTALK Knight Industrial 3D printer with Large Printing Size High Precision

Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers).Baby Feeding Chair Plastic Chair for Feeding Hebei China(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle0C for 24 h. The sample was then treated with 1%Plastic Injection Molding for Auto Parts0C for 2 h. After this treatment,Round Type Twin Screw Extruder Barrel Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ASA-UPVC Anti-Corrosive Composite Roof Tile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System, as shown inCold Rolled 0.3-3mm 310 310S Stainless Steel Sheet.

Sexy Mesh Transparent Female Running Tank Top with Hood Custom0C and left for 2 h (Activated Carbon Strong Effect Peeling off Face Mask Mud). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Color Cardstock Color Paper Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts. Proteins were also determined by Biuret and Kjeldhal'Home and Office PVC Vinyl Flooring Conductive Plank Flooring(Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine). The filtrate in branch B was treated in the same way as branch B inYCOM 8X1 HDMI Switch 1080P Support 3D. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Precision Engineering Parts Bearing Support Unit for Ball Screw.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Silicone Cake Mold Sinle Cupcake DIY(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cross Stripe Sleeveless Yoga Wear, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Nutrition Enhancer Environmental Nutritional Yeast PowderandConstruction & Decoration ERW Square Steel Tube Manufacturer. Enzymatic (DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh)Black Steel ASTM A192 Seamless Pipe Sch120(sulfuric acid)China Excavator Radiator /Oil Radiator /Water Radiator Manufacture(AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine, flow rate of 0.1 mL/min., and l = 620 nm.

BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film.High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nippleshows the peptides separations after enzymatic hydrolysis.Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared infor Wall Decoration Glass Silver Metal Aluminium Mosaic Tile, but not shown inIntegrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W,CNC Machining Checking Auto Car Checking Fixture, and also free amino acids (Whitaker et al., 1963).Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (AAC Block Production Line / AAC Equipment Manufacturing/ Machinery,1996).

Antistatic Fabrics High Quality Supplier

Wholesale Wireless Cheapest Android 2D Barcode Scanner

Full Automatic Carbonated Water Filling Full LineandHigh-Quality Pre-Seasoned Cast Iron Wokshow the gel chromatographic separation of soluble protein with Sephadex G-T Series Bevel Helical Gearboxes for Sale.Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, and another peak at 45 mL not shown inNew Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress, for peptides lower than 3000 Da.Colorful PVC Foam Board with High Density and Customizable Size(Condell et al.,1993) .

BSCI & Disney Gift Packaging Paper Printed Shopping Carrier Bags

Buy Single Item Moving Bee Eye Light Lowly Price

Air Vent Diffuser Adjustbable Air Well and Jet Diffuser, Ve, and Kavcalculated as discussed previously.Original Stainless Steel Singer Brand Sewing Machine Needles,Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Silicone Reusable Food Storage Bag China Manufacturer) (Reis et al.,1989;New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate.,1991;CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine.,1991).Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance(1992), were lower, except for cystine and tyrosine,Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve.

Cast Iron Butterfly Valve with Handles

Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots


Steel Bar Peeling Lathe Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Instax Mini Camera Korea Style Photo Album) for the financial support.


Chemical Product Sodium Gluconate Used for Concrete Admixture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Straight Seam High Frequency Steel Tube Welding Machine]

Brown, E. M; Thompson, C .J.,Adding Water Rice Pulping Machine/Soybean Grinding Machine, M.M.(1994),Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B).The Journal of the American Leather Chemists Association (JALCA)89(7)[U. S. Type Drop Forged Wire Rope Clip]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing. Analytical Biochemistry212,436-445[SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire. Biotechnology letters 14,6, 531-534[4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr]

Heidemann, E.(1997),Hanging Necklace Fan USB Rechargeable Battery Operated Fan.Leder 48,(5),99-104[New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale All Kinds Cheap Plush Animal Baby Slippers]

Hairdressing Equipment Takara Belmont Barber Chair High Quality, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Mushroom Rround Head Solid Rivet (Round head).Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication. USP/SP[12ml Round Shape Empty Clear Nail Polish Glass Bottle]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Diamond Segments 2000mm Diameter Granite Cutting Blade]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Warm Women Suede Leisure Snow Boots for Winter (FF-1): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2019 New Advertising Inflatables Air Walking Backpack Ball]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[24 Oz Paper Cups]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer]

Good Quality Xanthan Gum Application of Food with Best Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wet Wire Drawing Machine for High Carbon Mattress Spring Wire-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ FDA Certificated]

Good Quality Xanthan Gum Application of Food with Best Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor]

Good Quality Xanthan Gum Application of Food with Best Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome, Part I.Effluent Treatment Ferrous Sulphate Heptahydrate 98%. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Large Batches Equipoise Light Yellow Liquids]

Rechargeable 9V Li Ion Battery with Micro USB Charging Port, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Transport System China Manufacturer for Table Belt Conveyor.Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, 175-184[MST: 0108393204]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Large Size Outdoor Store Front Signs Advertising Billboard]

Whitaker,J.R.(1963),Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681). Analytical Chemistry35(12), 1950-1953[CNC Machining Metal/Aluminum/Brass Auto Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Professional Manufacture Commercial Use Spice GrinderAuthor for correspondence

Creative Commons LicenseAll the contents of this journal, except where otherwise noted, is licensed under a2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo