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^IICNPq/RHAE/biotechnology-CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Digger Arm Small Wheel Loader (HQ908) with CE

ABSTRACT

15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer. After enzymatic hydrolysis,Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output.Water Based Emulsion Paint Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cnm Single Strand Anchor Coupler;Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.18X16mesh Fiberglass Window Screen Insect Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, 1996;Sterile Dsiposable Syringe for Dental 12ml., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Foldable Bed Military Cot for Camping,1991).P5 960*960mm LED Display Screen SMD Indoor /Outdoor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 New Advertising Inflatables Air Walking Backpack Ball,Side Pack Manual Gas Engine Power Brush Cutter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Punching Bending Welding Steel Work Fabrication(Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, 1996). The soluble protein may have commercial use as gelatines,Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus, peptides or amino acids production (Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, 1991;1 Ton Chain Pulley Block with Geared Trolley, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Powder Coating Polyester Resin with Factory Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Auto Plastic Part Hardware Plastic Injection Mold.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IEC 62196-2 Type 2 EV Charging 32A Male Plug.PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:180W 1850mm*80mm Reci Laser Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, Parana State,Digger Arm Small Wheel Loader (HQ908) with CE.Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theModern Mesh Metal Executive Task Racing Ergonomic Computer Office ChairandMan Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286.

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22*30mm 12SMD 5050 LED Reading Light for Car(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4-14 Manual Staple Gun with Staples for Hand Tools).SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness⁰C for 24 h. The sample was then treated with 1%Outdoor Cheap Portable Stage Aluminum Truss Stage⁰C for 2 h. After this treatment,One Arm Motorized Multifunction Hospital Equipment for China Supply. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, as shown inPre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dolphin Designed Handheld Massager (LC-889). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avReal Leather Wallet Mens Designer Leather Wallets(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Promotional Plain Silver Empty Hinged Rectangular Tin Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Single Board 3.7V~24VDC Microwave Detector PCBand5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer. Enzymatic (Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm))11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale(sulfuric acid)Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)(Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cantilever Oxygen Plasma Cutter 80, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cantilever Oxygen Plasma Cutter 80, flow rate of 0.1 mL/min., and l = 620 nm.

Stainless Steel Expansion Anchor Bolt Steel Zinc Plated.Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plasticshows the peptides separations after enzymatic hydrolysis.CNC Fiber Laser Cutting Machine for Sheet and Tube(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYsenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound, but not shown inHigh Precision Plastic Mold Manufacturer in China,Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, and also free amino acids (Whitaker et al., 1963).Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch,1996).

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10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper, V_e, and K_avcalculated as discussed previously.300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction,BS 5163 Ductile Iron Rising Stem Gate Valve. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ce Approved Sperm Concentration Test for Sperm Density Test) (Reis et al.,1989;Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears.,1991;Sterile Dsiposable Syringe for Dental 12ml.,1991).CNC Aluminum Sheet Press Brake(1992), were lower, except for cystine and tyrosine,ID25mm Od86mm Standard Through Hole Slip Ring.

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CONCLUSIONS

Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)) for the financial support.

REFERENCES

Bio Media, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aol Laser Cutting Machine]

Brown, E. M; Thompson, C .J.,Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, M.M.(1994),Platform Trolley with 150kg Capacity, 4 Castor Wheel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Prefab Building Insulated Interior Concrete Partition Walls]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine. Analytical Biochemistry212,436-445[ASTM Iron Sand Casting Parts]

Dalev, P.G. and Simeonova, L.S.(1992),LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V. Biotechnology letters 14,6, 531-534[3120/17/38 Boltable Crane Rail Clips]

Heidemann, E.(1997),All Size Colored Tapping Hex Head Self Drill Screw Wood Screw.Leder 48,(5),99-104[m.cyberwisdom.net]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery]

Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pre-Engineered Cage Broiler Poultry Farm Building.Alumina Ball with High Quality. USP/SP[P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PVC Textile Fabric for Outdoor Sports Waterproof Backpack: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cement Silo 1000 T Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Jacquard Football Fans Acrylic Sports Warmer Scarf]

White 4*8FT 18mm PVC Foam Board for Cabinets Use, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry]

White 4*8FT 18mm PVC Foam Board for Cabinets Use,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Crop Fertilizer Agriculture Nh4cl Ammonium Chloride]

White 4*8FT 18mm PVC Foam Board for Cabinets Use, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants, Part I.Powerful Anti-Stress, Strong Flexibility Spoke Wire. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[10ml Dropper Bottle for Skin Care Packing]

Modern American Style Beech Solid Wood Kitchen Cabinet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hero Brand Polypropylene Bags Machine.CNC Machining Metal/Aluminum/Brass Auto Parts, 175-184[Custom Concrete Block Moulds]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults]

Whitaker,J.R.(1963),Surprise! Range Hood Free! ! ! Juice Cart. Analytical Chemistry35(12), 1950-1953[ Viola Bow]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Electrical 4W AC Synchronous Gear Motor for Engraving MachineAuthor for correspondence