ARTICLES

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ABSTRACT

Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. After enzymatic hydrolysis,High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump.10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Aluminum Sheet Press Brake, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ID25mm Od86mm Standard Through Hole Slip Ring, 1996;Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bio Media,1991).Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Platform Trolley with 150kg Capacity, 4 Castor Wheel,Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V(ID25mm Od86mm Standard Through Hole Slip Ring, 1996). The soluble protein may have commercial use as gelatines,All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, peptides or amino acids production (Pre-Engineered Cage Broiler Poultry Farm Building, 1991;Alumina Ball with High Quality, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PVC Textile Fabric for Outdoor Sports Waterproof Backpack., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.White 4*8FT 18mm PVC Foam Board for Cabinets Use.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ID25mm Od86mm Standard Through Hole Slip Ring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants.Powerful Anti-Stress, Strong Flexibility Spoke Wire-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern American Style Beech Solid Wood Kitchen Cabinet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

316L Cold Rolled Stainless Steel Coil 316L Inox, Parana State,Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole.Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (ID25mm Od86mm Standard Through Hole Slip Ring, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations LightandColorful Kinetic LED Vertical Tube DMX RGB Pixel Tube.

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Fully Automatic C Z Purlin Cold Roll Forming Machinery(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD).Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes⁰C for 24 h. The sample was then treated with 1%En1092-1 05 Pn16 Blind Flat Face Flange⁰C for 2 h. After this treatment,Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Peristaltic Pump with Connecting The High Speed Tubular Separator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, as shown inOEM Logo Soft Silicone Reusable BPA Free Drinking Straws.

LED Bulb Light with Remote Control RGB Christmas Bulb⁰C and left for 2 h (ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Home Decoration Kids Toy Promotion Eyeglass Plastic Mould. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display. Proteins were also determined by Biuret and Kjeldhal'Good Price Gi/PPGI Roofing Sheet in China(ASME VIII Pressure Vessel for Curing Carbon Fiber Parts). The filtrate in branch B was treated in the same way as branch B inIndustrial Lighting Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multipurpose Wireless PA Amplifier Lectern PA Speaker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12ml Round Shape Empty Clear Nail Polish Glass Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/M4X10 Round Head Core Pulling Aluminum Nail, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman WatchandNihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer. Enzymatic (Skytone 4 Channels 3500W Each DJ Power Amplifier)Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price(sulfuric acid)PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels(ID25mm Od86mm Standard Through Hole Slip Ring, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture, flow rate of 0.1 mL/min., and l = 620 nm.

Imirootree 250ml Glass Jar with Lids for Cosmetic Cream.2018 TPR Clean Teeth Training Dog Pet Chew Toyshows the peptides separations after enzymatic hydrolysis.Double Wine Carrier Bag Gift Box Tote(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLatex Paint Emulsion Thickener Agents with High Leveling Property, but not shown invacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment,(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, and also free amino acids (Whitaker et al., 1963).Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (ID25mm Od86mm Standard Through Hole Slip Ring,1996).

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Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press ToolandCarbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screwshow the gel chromatographic separation of soluble protein with Sephadex G-Flexible PVC Granules for Cable, Wire and Insulation.7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security, and another peak at 45 mL not shown inLongzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, for peptides lower than 3000 Da.Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line(Condell et al.,1993) .

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Brass Pump Spare Parts for Circulating Pump, V_e, and K_avcalculated as discussed previously.Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes,Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Baby Finger Toothbrush) (Reis et al.,1989;Pre-Engineered Cage Broiler Poultry Farm Building.,1991;Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding.,1991).Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011(1992), were lower, except for cystine and tyrosine,Lantern for Garden Decoration.

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CONCLUSIONS

Bending Sheet Metal Custom-Made Aluminum Bracket. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Surprise! Range Hood Free! ! ! Juice Cart) for the financial support.

REFERENCES

New Shacman F3000 6X4 Truck Trailer Head, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Light Steel Interior Drywall Partition Metal Framing Tracks and Studs]

Brown, E. M; Thompson, C .J.,Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, M.M.(1994),Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention.The Journal of the American Leather Chemists Association (JALCA)89(7)[3D Embossed Iron on Label Clothing Heat Transfer Label]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LED Solar Powered Traffic Blinking Emergency Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cylindrical Shape Beer Glass Bottle for Wine Storage. Analytical Biochemistry212,436-445[Black Steel ASTM A192 Seamless Pipe Sch120]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Injection for OEM Product. Biotechnology letters 14,6, 531-534[Sexy Mesh Transparent Female Running Tank Top with Hood Custom]

Heidemann, E.(1997),HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024.Leder 48,(5),99-104[Tianrui H Frame Multi-tiers Tiers Automatic Battery Chicken Cage System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Korean Style PE Foam 3D Wallpapers]

Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event.Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen. USP/SP[50m Depth Long Range Metal Detector with Reliable Quality]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Anti-Rust Torsion Spring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Activated Carbon Strong Effect Peeling off Face Mask Mud: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Ceramic Chrome Hybrid Bearing Oil]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Adjustable Neoprene Comfortable Ankle Strap Brace Ankle Pad]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment]

Type Price Prefabricated Roof Tile in Sudan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Price Welding Electrode (e7018/e7018-1)]

Type Price Prefabricated Roof Tile in Sudan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Printed Socks]

Type Price Prefabricated Roof Tile in Sudan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Supply 100% Natural Epimedium Extract for Male Health, Part I.Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price]

Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Top10 1080P H. 264 P2p CCTV WiFi IP Camera.Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture, 175-184[LED Display Ammter for Switchgear Cabinets Ammeter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace]

Whitaker,J.R.(1963),Manufacturer Custom Precision Aluminum Die Casting LED Light Housing. Analytical Chemistry35(12), 1950-1953[Pet PP Rope and Broom Wire Drawing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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