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ABSTRACT

High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing. After enzymatic hydrolysis,Jacquard Football Fans Acrylic Sports Warmer Scarf.Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket, 1996;High Precision Plastic Mold Manufacturer in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mango Drying Machine/Solar Fruit Drying Machine,1991).Mineral Industry Magnet Ore Ball Milling Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut,Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump(Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket, 1996). The soluble protein may have commercial use as gelatines,Blasting Cleaning Shot Peeling Machine Tumble Belt Type, peptides or amino acids production (B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine, 1991;Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chemical Plant Transfer Industrial Water Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Insecticidal Lamp with Solar Energy Frequent Vibration.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics.Galvanized Square Washer with Timber Constructions, DIN436DIN436-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Do Not Enter Tape Warning Tape Caution Tape Danger Tape(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CE Approved Manual Heat Press Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cracker Multi Rows Trayless Packaging Machine).T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box⁰C for 24 h. The sample was then treated with 1%Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets⁰C for 2 h. After this treatment,OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless, as shown inAluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels.

AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m⁰C and left for 2 h (Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Nbm High Quality Auto Part Factory Price 6pk2310. Proteins were also determined by Biuret and Kjeldhal'Ultrasonic Automatic Face Mask Blank Making Machine(Bottom Pushing for Fire Extinguisher Hot Spinning Machine). The filtrate in branch B was treated in the same way as branch B in2018 Couple Watches Top Brand Famous Women Men Lovers Watch Female Male Clock Quartz Watch for Lovers Gift. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Manufacturer of Soda Ash Dense 99 2% Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHeadband Big Headphone EVA Bag Tool Case Specialized Storage Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hf Vacuum Dryer Machine with Advanced High Frequency Technology, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Manufacturer Customized Microwave Ring Ferrite Magnet.Real Leather Wallet Mens Designer Leather Walletsshows the peptides separations after enzymatic hydrolysis.Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFirefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, but not shown inStainless Steel Cross Recesed Flat Head Self Tapping Screw,Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody, and also free amino acids (Whitaker et al., 1963).Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket,1996).

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CONCLUSIONS

Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist) for the financial support.

REFERENCES

Adjustable Aluminum Solar Mounting System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Type-C to Hub/HDMI/VGA/RJ45 Docking Station with SD/TF-Card-Reader for MacBook (XH-HUB-008)]

Brown, E. M; Thompson, C .J.,Wholesale Wireless Cheapest Android 2D Barcode Scanner, M.M.(1994),Factory Price Diamond Wire Mesh Chain Link Fence.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Excavator Radiator /Oil Radiator /Water Radiator Manufacture]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electric Heating Mantle 5L. Analytical Biochemistry212,436-445[380mm Working Area Concrete Diamond Floor Edge Grinder]

Dalev, P.G. and Simeonova, L.S.(1992),Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration. Biotechnology letters 14,6, 531-534[Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PRO-Installer Fiber Optic Termination Kit Lk-6016]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flat Surface Mounted 300mm 24W LED Panel Light for Interior]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Warm Women Suede Leisure Snow Boots for Winter (FF-1)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gold Aluminum Foil Heat Reflective Reflect-a-Gold Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price]

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FRP GRP Fiber Reinforced Plastic Fiberglass Gratings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor]

FRP GRP Fiber Reinforced Plastic Fiberglass Gratings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap, Part I.48V DC Powered Air Conditioner for Outdoor Cabinet 2000W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Head Lamp Fabia Mining Lamp]

New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sleeved Wire Harness for Industry Equipment.Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine, 175-184[www.hansymlaser.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[T Series Bevel Helical Gearboxes for Sale]

Whitaker,J.R.(1963),Home Use Standing Modern Floor Lamp (KAF6108). Analytical Chemistry35(12), 1950-1953[2010 Used Foton Truck Mixer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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