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ABSTRACT

IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker. After enzymatic hydrolysis,Customized Stable Burn-in Test Equipment.Wholesale Custom 100% Cotton Boys T-Shirt with Print. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Industry 0.002mm Accuracy Checking Fixture;Promotional Customized PP Hand Fan, Plastic Folding Fan; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Six Stations Pen Press Machine, 1996;FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Vertical Automatic Granule Bag Packing Machine,1991).Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures,4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price(Six Stations Pen Press Machine, 1996). The soluble protein may have commercial use as gelatines,Small Manufacturing Tht/ Ai Double Wave Soldering Machine, peptides or amino acids production (Manual Sealing Machine for Instant Noodles Paper Soup Bowls, 1991;Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Corrugated Steel Sheet Corrugated Steel Roofing Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Six Stations Pen Press Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Factory Queen Mattress with Visco Memory Foam and Latex.High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine, Parana State,CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040).Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Six Stations Pen Press Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAuto Plastic Part Hardware Plastic Injection MoldandHerbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine.

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0.1mm Tungsten Carbide 2f Single Flute Micro End Mill(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital).Agriculture Large Span Commerical Steel Frame Greenhouse(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat⁰C for 24 h. The sample was then treated with 1%14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags⁰C for 2 h. After this treatment,Ss400 Corner Metal Sheet Bar Galvanized Angle Iron. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, as shown inBest Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector.

Chinese Jbcz Handy Operation Threading Machine and Coupler⁰C and left for 2 h (60m3/H Lamella Clarifier for River Water Treatment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Promotional Reusable Foldable PP Non Woven Shopping Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board. Proteins were also determined by Biuret and Kjeldhal'Factory Price Printing Doy Pack Bag(DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut). The filtrate in branch B was treated in the same way as branch B inEgg Tray Making Machine Project Report. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDirect Factory Tebuconazole Price Fao Bactericide Tebuconazole Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Engine Parts Rubber High Quality Timing Belt 111ru25⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Interior Metal 3D Bee Garden Ornament Decoration, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Starch Doy Bag Packaging MachineandDisposable Non-Woven Dry Gloves Wipes Making Machine. Enzymatic (ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting)Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65(sulfuric acid)Bottom Pushing for Fire Extinguisher Hot Spinning Machine(Six Stations Pen Press Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fish Scales Texture Fashion Leather for Shoe (S312100GH), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fish Scales Texture Fashion Leather for Shoe (S312100GH), flow rate of 0.1 mL/min., and l = 620 nm.

Diamond Segments 2000mm Diameter Granite Cutting Blade.2 Quick Change Flexible Roloc Discshows the peptides separations after enzymatic hydrolysis.Produce N2o for Medical Gas Equipments(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHolo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, but not shown inSoldering Machine LED Module PCB Hot Air Soldering Station,Hot Selling Furring Channel Fittings Suspended Ceiling Accessories, and also free amino acids (Whitaker et al., 1963).90m3 Per Hour Mobile Concrete Pump. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Six Stations Pen Press Machine,1996).

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20%ScandManufacturer of Soda Ash Dense 99 2% Priceshow the gel chromatographic separation of soluble protein with Sephadex G-China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter.FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, and another peak at 45 mL not shown inBarbed Wire Right off The Border Wall to Protect Homes, for peptides lower than 3000 Da.All Weather Rattan Handwoven Swing Outdoor Home(Condell et al.,1993) .

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CONCLUSIONS

School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems) for the financial support.

REFERENCES

Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Melamine Foam Sheet]

Brown, E. M; Thompson, C .J.,Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation, M.M.(1994),0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-125kHz RFID Hotel Key Card with T5577 Chip. Analytical Biochemistry212,436-445[Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. Biotechnology letters 14,6, 531-534[FTTX Solution Provider Fiber Optical 12cores MPO Cassette]

Heidemann, E.(1997),Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag.Leder 48,(5),99-104[Factory Price Vulcanizing Press Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.]

Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Crop Fertilizer Agriculture Nh4cl Ammonium Chloride.Wholesale High Pressure 80L Nitrogen Gas Cylinder. USP/SP[Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Instax Mini Camera Korea Style Photo Album]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10mm Red Green Yellow Warning China Supplier Signal Lamp: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sterile Dsiposable Syringe for Dental 12ml]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Alarm Full Body Detector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner]

Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Thermostat Electric Heating Element for Solar Water Heaters-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industrial Rubber Nylon Conveyor Belting Automotive Parts]

Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump]

Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Phillip Countersunk Head Screw Hot DIP Galvanized, Part I.Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PP Woven Bag]

Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heat Shrink Automatic Flow Wrapping Packing Machine.Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, 175-184[Zinc Sulfide Material Zns Granules for Optical Coating]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ASTM A240 321 Stainless Steel Sheet Price Per Kg]

Whitaker,J.R.(1963),Ductile Iron Standard Brackets Pipe Hanger. Analytical Chemistry35(12), 1950-1953[ CNC Lathe Machine Milling]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Bending Sheet Metal Custom-Made Aluminum BracketAuthor for correspondence