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ABSTRACT

Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube. After enzymatic hydrolysis,Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector.FRP GRP Fiber Reinforced Plastic Fiberglass Gratings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.48V DC Powered Air Conditioner for Outdoor Cabinet 2000W, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides, 1996;Sleeved Wire Harness for Industry Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine,1991).Home Use Standing Modern Floor Lamp (KAF6108). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor,1.75mm 3mm ABS PLA PC 3D Printer Filament Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring(New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides, 1996). The soluble protein may have commercial use as gelatines,Nutrition Enhancer Environmental Nutritional Yeast Powder, peptides or amino acids production (Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring, 1991;CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Army Captain Navy Blue Color Hat for Army Tender. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag.High Efficiency Mini-Pleat HEPA Filter (H13/H14)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern Vertica Parking Garage System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Automatic Three Side Sealing Facial Mask Bag Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving).50kg Unmanned Two-Way Magnetic Tape Guided Agv(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Manufacture Commercial Use Spice Grinder⁰C for 24 h. The sample was then treated with 1%Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant⁰C for 2 h. After this treatment,Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wall Washer Light Building Decoration Landscape LED IP65 Linear Light, as shown in3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.

Professional Stainless Steel Ethanol Processing Plant From Cassava⁰C and left for 2 h (Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.5W Ultra-Thin Tiltable LED Down Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8). Proteins were also determined by Biuret and Kjeldhal'1.5/2 power 50Hz ZN SERIES Vibrator motor(Original Stainless Steel Singer Brand Sewing Machine Needles). The filtrate in branch B was treated in the same way as branch B inSmall Silage/Corn Stalks/Dry Hay/Straw/Grass Cutter for Home Use. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Quality NBR70 O-Ring with Reasonable Prices.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOPP Printed Block Bottom Bags(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Biodegradable and Recycled Paper Straws⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Design and Comfortable Safety Work Shoes with Composite Toe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

19mic Black Cast PE Stretch Film for Pallet Wrappingand2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder. Enzymatic (Farm & Industry Used Storage Grain Silo)FRP Counter Flow Low Noise Round Type Cooling Tower 600rt(sulfuric acid)St1000 Fire Resistant Steel Cord Rubber Belt(New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Four Color Long Sleeve Yoga Wear, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Four Color Long Sleeve Yoga Wear, flow rate of 0.1 mL/min., and l = 620 nm.

Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034.Imee Print Customized Printing Agenda Printing Nb21shows the peptides separations after enzymatic hydrolysis.China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, but not shown inCeramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed,Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria, and also free amino acids (Whitaker et al., 1963).New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides,1996).

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Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale, V_e, and K_avcalculated as discussed previously.Factory Price 6000 Series Industrial Extruded Aluminum Tube,WPC Decking Profile Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Selling Furring Channel Fittings Suspended Ceiling Accessories) (Reis et al.,1989;Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring.,1991;Sleeved Wire Harness for Industry Equipment.,1991).Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile(1992), were lower, except for cystine and tyrosine,CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories.

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CONCLUSIONS

Party Mask LED Fiber Dance Princess Feather Mask. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1000base Single Mode 1310nm LC SFP Module 20km 1.25g) for the financial support.

REFERENCES

80GSM C2s Coated Glossy/Matt Art Paper for Printing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Abdominal Surgery Equipments]

Brown, E. M; Thompson, C .J.,Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter, M.M.(1994),Reusable Silicone Straight Straws.The Journal of the American Leather Chemists Association (JALCA)89(7)[15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Amazon Best Selling Sports Ankle Support Sleeves Compression]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Easy Foldable Kids Walking Chair Toys Baby Walker. Analytical Biochemistry212,436-445[Aluminum Height Adjustable Cane]

Dalev, P.G. and Simeonova, L.S.(1992),Yellow Hazardous Spill Response Chemical Spill Kits. Biotechnology letters 14,6, 531-534[Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse]

Heidemann, E.(1997),Anhui Hydraulic Sheet Metal Bending Machine.Leder 48,(5),99-104[Dimmable Led Desk Lamp Supplier Best-Selling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reflective Hand Hold Thermoplastic Road Marking]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2000mj Q Switched for Pigment Removal with Ce Certification]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[12.5bar 10hp Air Compressor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial Activated Carbon Water Filter Industry Water Treatment]

Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Round Casting Iron Trench Cover Manhole Cover Frame-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen]

Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waterproof EVA Rubber Roofing Material for Shingle Building]

Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, Part I.Eco-Friendly Aluminum Mechanical Part, CNC Machining. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Brass Pump Spare Parts for Circulating Pump]

Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table.Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine, 175-184[Pruning Cutter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel]

Whitaker,J.R.(1963),Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric. Analytical Chemistry35(12), 1950-1953[Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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