ARTICLES

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ABSTRACT

High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17. After enzymatic hydrolysis,Engine Parts Rubber High Quality Timing Belt 111ru25.Wholesale Interior Metal 3D Bee Garden Ornament Decoration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket;Disposable Non-Woven Dry Gloves Wipes Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, 1996;Bottom Pushing for Fire Extinguisher Hot Spinning Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fish Scales Texture Fashion Leather for Shoe (S312100GH),1991).Diamond Segments 2000mm Diameter Granite Cutting Blade. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Produce N2o for Medical Gas Equipments,Hot Selling Furring Channel Fittings Suspended Ceiling Accessories,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,90m3 Per Hour Mobile Concrete Pump(Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, 1996). The soluble protein may have commercial use as gelatines,China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter, peptides or amino acids production (FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, 1991;All Weather Rattan Handwoven Swing Outdoor Home, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pond Self Priming Booster suction  Water Pump with Pressure Switch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile.100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Crop Fertilizer Agriculture Nh4cl Ammonium Chloride(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Custom 100% Cotton Boys T-Shirt with Print).Wholesale High Pressure 80L Nitrogen Gas Cylinder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%10mm Red Green Yellow Warning China Supplier Signal Lamp⁰C for 24 h. The sample was then treated with 1%Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor⁰C for 2 h. After this treatment,Hot Sale Thermostat Electric Heating Element for Solar Water Heaters. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Phillip Countersunk Head Screw Hot DIP Galvanized's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, as shown inT6s 86 PCD Core Bit for Geotechnical Drilling.

Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll⁰C and left for 2 h (190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Heat Shrink Automatic Flow Wrapping Packing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. Proteins were also determined by Biuret and Kjeldhal'Ductile Iron Standard Brackets Pipe Hanger(Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles). The filtrate in branch B was treated in the same way as branch B inUV LED 365nm Curing System 200W Lamp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Different Size Glass Test Tubes, High Quality Laboratory Test Tubes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Frame Type Rubber Hydraulic Press Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Flooded Type Water Cooled Screw ChillerandKitchen Metal Dish Storage Rack (JP-SC984C). Enzymatic (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming)AVR Se350 Automatic Voltage Regulator for Marathon Alternator(sulfuric acid)Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage(Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, flow rate of 0.1 mL/min., and l = 620 nm.

High Strength Rigid PVC Plastic Foam Board for FRP.Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tapeshows the peptides separations after enzymatic hydrolysis.Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Size Christmas Gift Personalized Watch Boxes for Men, but not shown inGood Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines,4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, and also free amino acids (Whitaker et al., 1963).Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65,1996).

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Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, V_e, and K_avcalculated as discussed previously.Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module,ADSS Newest Ultra Therapy Skin Tightening 3D Hifu. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner) (Reis et al.,1989;FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node.,1991;Bottom Pushing for Fire Extinguisher Hot Spinning Machine.,1991).Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap(1992), were lower, except for cystine and tyrosine,Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon.

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CONCLUSIONS

High Quality Ceramic Chrome Hybrid Bearing Oil. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine) for the financial support.

REFERENCES

Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Glass Clamp/Glass Clip]

Brown, E. M; Thompson, C .J.,CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder, M.M.(1994),Digger Arm Small Wheel Loader (HQ908) with CE.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Step Motor for Intelligent Sanitary/3D Printer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer. Analytical Biochemistry212,436-445[Swimming Pool Water Disinfect Ozone Generator]

Dalev, P.G. and Simeonova, L.S.(1992),Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output. Biotechnology letters 14,6, 531-534[Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing]

Heidemann, E.(1997),Water Based Emulsion Paint Production Line.Leder 48,(5),99-104[4 Wheels Abdominal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Glass Door Passenger Sightseeing Elevator for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-18X16mesh Fiberglass Window Screen Insect Screen: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mini Bluetooth Speaker With Led Touch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Lead Ingot 99.994 Reasonable Price]

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Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle]

Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Foldable Bed Military Cot for Camping, Part I.P5 960*960mm LED Display Screen SMD Indoor /Outdoor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bearing Stand Type Cam Follower]

2019 New Advertising Inflatables Air Walking Backpack Ball, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Side Pack Manual Gas Engine Power Brush Cutter.Punching Bending Welding Steel Work Fabrication, 175-184[Pet Flakes Centrifugal Drying Dewatering Machine/Plastic Bottle Recycling Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Vacuum Squeeze Filter Press for Coal Flotation Tailings]

Whitaker,J.R.(1963),Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus. Analytical Chemistry35(12), 1950-1953[Cracker Multi Rows Trayless Packaging Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite PanelsAuthor for correspondence