ARTICLES

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ABSTRACT

Wear Resitant Rope End Cap Plastic Flange Cover. After enzymatic hydrolysis,Comfortable Two Piece Women Fitness Clothing for Gym Wear.Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Orfs Male/Orfs Female Hose Fitting, 1996;Ss400 Galvanized Steel C Channel Steel Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping,1991).ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple,High Quality Step Motor for Intelligent Sanitary/3D Printer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,81144m Thrust Roller Bearing Used for Vertical Machine Machinery(Orfs Male/Orfs Female Hose Fitting, 1996). The soluble protein may have commercial use as gelatines,Fancy Veneer Plywood. Natural Veneer Plywood for Furniture, peptides or amino acids production (OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, 1991;Aluminum Foil Heat Shield Screen Matting Adhesive Backed, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Home Kitchen Water Purification Ozone Generator 1000mg., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PP Foam Stationary Sheet Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Orfs Male/Orfs Female Hose Fitting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Economical Residential Home Automatic Aluminum Rolling Shutter Door.Clear PVC Sheet with Coating for Card-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Classic Black and White Plaid Sling Bag for Women(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

100% Acrylic Knitted Sports Woven Scarf, Parana State,SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving.Prefab Steel Structure Shed for Storage Warehouse, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Orfs Male/Orfs Female Hose Fitting, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light FixturesandLine Scan Uvss System Portable Under Vehicle Surveillance.

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Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electric 100W Three Wheels Toy Car for Sale).Gen 2+ or 3 Day/Night Vision Scope(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved⁰C for 24 h. The sample was then treated with 1%3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA⁰C for 2 h. After this treatment,1650mm*80mm Stable Power Laser Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Lead Ingot 99.994 Reasonable Price, as shown in2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life.

Micro-Combustion Tube, Test Tube (HK-224)⁰C and left for 2 h (Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Service Machining Milling Turning Service Custom CNC Aluminum Case. Proteins were also determined by Biuret and Kjeldhal'3W LED Explosion Proof Emergency Exit Light(Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication). The filtrate in branch B was treated in the same way as branch B inWholesale Custom Aluminum Flag Pole with Best Quality. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stationery PP Clipboard With 7 Inch Scale Ruler. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Food Cookies Cake Cartoning Machine for Big Size Carton Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PVC Cross Joint Pipe Fittings Equal Pipe Cross⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3PC Stainless Steel Flanged End Ball Valve 3 Inches 150, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ysdcnc Manual Sheet Metal Plate Bending Roller Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Bio Media

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CONCLUSIONS

304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Free Sample E Liquid/E-Juice for Nicotine Vaping) for the financial support.

REFERENCES

SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bath Mat Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, M.M.(1994),Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut.The Journal of the American Leather Chemists Association (JALCA)89(7)[Yashi 19 270degree Pyramid Hologram Display Showcase Attractive]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Portable Electric Concrete Vibrator with 18000 High Rotation Speed]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts. Analytical Biochemistry212,436-445[Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant)]

Dalev, P.G. and Simeonova, L.S.(1992),Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine. Biotechnology letters 14,6, 531-534[China Supplier Rubber Injection Molding Machine with Ce]

Heidemann, E.(1997),304 316 Stainless Steel Grooved Cross for Water.Leder 48,(5),99-104[China Automobile Parts Sourcing High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom High Precision Micro Machining Service Brass Micromachining Parts]

High Quality Crusher for Used Plastic Recycling Line, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole.Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home. USP/SP[Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Three Side Sealing Facial Mask Bag Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Steel Bar Binding Hook]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply]

Hot Sale Laser Projector RGB Laser Light for Stage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple]

Hot Sale Laser Projector RGB Laser Light for Stage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding]

Hot Sale Laser Projector RGB Laser Light for Stage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, Part I.Acm Dewatering Twin Screw Extruder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof]

Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tumble Belt Type Shot Blasting Machine for Iron Castings Parts.Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple, 175-184[Cast Iron Round Mini Skillet with Handle 6.5]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory]

Whitaker,J.R.(1963),Good Quality Good Price China Plastic Injection Molding Machine. Analytical Chemistry35(12), 1950-1953[Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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