ARTICLES

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Christmas TiarasEdivaldo E.Garcia^I; Cláudia C.M.CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Chicken Layer Cage Poultry Cage Chicken Cages}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hot Sale Biodegradable and Recycled Paper Straws. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair

ABSTRACT

Fashion Design and Comfortable Safety Work Shoes with Composite Toe. After enzymatic hydrolysis,2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder.Farm & Industry Used Storage Grain Silo. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;FRP Counter Flow Low Noise Round Type Cooling Tower 600rt;St1000 Fire Resistant Steel Cord Rubber Belt; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Four Color Long Sleeve Yoga Wear, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, 1996;China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria,1991).New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,XL-21 Type OEM Motive Electrical Switchgear Power Cabinet,Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,White HPL 600*600mm Coffee Table(Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, 1996). The soluble protein may have commercial use as gelatines,Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale, peptides or amino acids production (Factory Price 6000 Series Industrial Extruded Aluminum Tube, 1991;WPC Decking Profile Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Party Mask LED Fiber Dance Princess Feather Mask. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰80GSM C2s Coated Glossy/Matt Art Paper for Printing.Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Reusable Silicone Straight Straws(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree, Parana State,Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair.1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special ThicknessandASA-UPVC Anti-Corrosive Composite Roof Tile.

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Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Promotional Reusable Foldable PP Non Woven Shopping Bag).Round Casting Iron Trench Cover Manhole Cover Frame(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane⁰C for 24 h. The sample was then treated with 1%Eco-Friendly Aluminum Mechanical Part, CNC Machining⁰C for 2 h. After this treatment,Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine, as shown inPVC Cross Joint Pipe Fittings Equal Pipe Cross.

Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric⁰C and left for 2 h (Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ductile Iron Flanged Swing Check Valve. Proteins were also determined by Biuret and Kjeldhal'Ozonated Water for Vegetables and Fruits Washing Ozone Generator(Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers). The filtrate in branch B was treated in the same way as branch B inBiological Students Microscope Set Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Day and Night Zebra Blind Fabric Z1602. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Impact Sound Isolation Wall Clip⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving, pH 3.25., mobile phase B: buffer solution of 0.01 mol/German Technology Automatic Stacking System in Red Brick Automatic Production Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

8p8c Mini RJ45 Female Jack Module Connector RJ45 ConnectorandTop Quality Galvanized Iron Wire as Binding Wire. Enzymatic (13.56 MHz Fudan FM11RF08 Chip Card)Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt(sulfuric acid)Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles(Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, flow rate of 0.1 mL/min., and l = 620 nm.

100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap.Modern American Style Beech Solid Wood Kitchen Cabinetshows the peptides separations after enzymatic hydrolysis.Square Premium Glass Perfume Bottle 100ml(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in8mm Heavy Duty Nails U Shaped Galvanized Staples, but not shown in6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower,Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, and also free amino acids (Whitaker et al., 1963).Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034,1996).

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Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, V_e, and K_avcalculated as discussed previously.Cast Iron Butterfly Valve with Handles,Furniture Grade E1 Glue Plywood with Competitive Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug) (Reis et al.,1989;Factory Price 6000 Series Industrial Extruded Aluminum Tube.,1991;China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes.,1991).28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer(1992), were lower, except for cystine and tyrosine,Underground Garden 6W High Power Outdoor LED Lawn Light.

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CONCLUSIONS

Stamping Metal Parts Manufacturer Sheet Parts Fabrication. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Anhui Hydraulic Sheet Metal Bending Machine) for the financial support.

REFERENCES

Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Custom Debossed Silicone Wristbands]

Brown, E. M; Thompson, C .J.,3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, M.M.(1994),Hexagonal Netting/ Gabion Box/ Gabion Basket.The Journal of the American Leather Chemists Association (JALCA)89(7)[Disk Filter System with Automatic Backwash Valves]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor Type 40kgs Movable Ice Maker for Store Use]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Different Thickness High Quality Aluminum Foil for Glass Bottle Lid. Analytical Biochemistry212,436-445[Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla. Biotechnology letters 14,6, 531-534[Cantilever Oxygen Plasma Cutter 80]

Heidemann, E.(1997),Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages.Leder 48,(5),99-104[Factory Direct Cheap Malleable Cast Iron Pipe Galvanized Tee]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Transparent/Clear BOPP Jumbo Roll]

CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration.Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. USP/SP[81144m Thrust Roller Bearing Used for Vertical Machine Machinery]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Ozone Disinfection Machine Ozone Generator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[XL-21 Type OEM Motive Electrical Switchgear Power Cabinet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Diesel Generator Portable]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hairdressing Equipment Takara Belmont Barber Chair High Quality]

Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PVC Profile with Net Making Machine Used for Window Sealing Profile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)]

Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sand Castings by Gray Iron Material]

Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, Part I.Bobby Flay Websites Restaurant Martha Stewart Designer Tray. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Must 4kw 2HP Pump Power Inverter Ags Bts Available]

Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber.Diameter 2.68m Paragraphed Autoclave for AAC Bricks, 175-184[1x4 Fiber Optic Splitter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top10 1080P H. 264 P2p CCTV WiFi IP Camera]

Whitaker,J.R.(1963),Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety. Analytical Chemistry35(12), 1950-1953[ CNC Lathe Machine Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Plastic Injection Molding for Auto PartsAuthor for correspondence