ARTICLES

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ABSTRACT

Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark. After enzymatic hydrolysis,Custom Grey Full-Zip Cotton Mens Hoodies.Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, 1996;Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase,1991).Fcy30 Transport Mini Tipping Dumper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park,Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Premium Grade Rubber Cloth Duct Tape(12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, 1996). The soluble protein may have commercial use as gelatines,Sud1000h Poly Welder Butt Fusion Machine, peptides or amino acids production (Customized Anti-Rust Torsion Spring, 1991;Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PP Woven Bag, Rice, Beans, Plastic Bag.China Professional Mold Factory Plastic Injection Mould Maker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

DIN Standard Gratings/Gavlanized Steel Grating, Parana State,304 &314 Stainless Steel Seamless Bending Tube.50mm Magnetic River Water Sewage Flow Meter for Acid, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the4 Drawer Steel Office Vertical Filing CabinetandInsecticidal Lamp with Solar Energy Frequent Vibration.

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PE Biodegradable Sheet Product Line/Single Screw Extruder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel).27L/Min Water Exchanger Heat Pump Mold Temperature Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Felt Belt for Textile Compactor⁰C for 24 h. The sample was then treated with 1%Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt⁰C for 2 h. After this treatment,Metal Soft Enamel Souvenir Metal Medal Sport Medallions. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)20L Water Tank Lid Mold 3D Printing Samples Available's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale High Quality Metal Car Mount Holder Phone Holder, as shown inSequins Butterfly Handmade Beads Embroidery Patch.

Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch⁰C and left for 2 h (20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pet Laminate Aluminium Foil/Pet+Alu+PE. Proteins were also determined by Biuret and Kjeldhal'6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale(50mm Magnetic River Water Sewage Flow Meter for Acid). The filtrate in branch B was treated in the same way as branch B inVape Tool Kit. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Design Multi-Color Silicone Coin Purse for Lady.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPortable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM), pH 3.25., mobile phase B: buffer solution of 0.01 mol/1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)andWater Treatment Anthracite. Enzymatic (Heat Treated Welding Protection Glassfiber Cloth Ht800)Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring(sulfuric acid)Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector(12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Heat Pump Non-Programmable HVAC Room Thermostat, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Heat Pump Non-Programmable HVAC Room Thermostat, flow rate of 0.1 mL/min., and l = 620 nm.

Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo.Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screenshows the peptides separations after enzymatic hydrolysis.Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House, but not shown in Family Party Form Camping Kitchen,Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer, and also free amino acids (Whitaker et al., 1963).Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor,1996).

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Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, V_e, and K_avcalculated as discussed previously.Polyester / Polypropylene Woven Geotextile for Road Construction,Granite Stone Jaw Crusher of Mining Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair) (Reis et al.,1989;Customized Anti-Rust Torsion Spring.,1991;Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple.,1991).Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor(1992), were lower, except for cystine and tyrosine,Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer.

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CONCLUSIONS

The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (24k Gold Whitening Anti-Spot Mask) for the financial support.

REFERENCES

Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Forged & Machined Parts]

Brown, E. M; Thompson, C .J.,Wholesale All Kinds Cheap Plush Animal Baby Slippers, M.M.(1994),Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car.The Journal of the American Leather Chemists Association (JALCA)89(7)[4X4 Wd Car Tail Light for Ranger 2012-2016]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane. Analytical Biochemistry212,436-445[P5 960*960mm LED Display Screen SMD Indoor /Outdoor]

Dalev, P.G. and Simeonova, L.S.(1992),Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine. Biotechnology letters 14,6, 531-534[Supply Christmas Lace Gold Silver Offray Silk Ribbon]

Heidemann, E.(1997),New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress.Leder 48,(5),99-104[Wholesale 7.2X4.5X4.7m Christmas Inflatable Santa Bouncer with Slide]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet]

Factory Supply Cheap Custom Bleached White Cotton Gloves, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale.Taiding Custom Tank Sheet Metal Fabrication. USP/SP[Good Price Nasal Paste Packaging Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Airtight Candy Bin with Scoop: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Teerte Square Flat Head Self Tapping Screw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[glpurifier88]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Sheet Metal Stamping Bending Product Manufacturing Service]

Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Water Chiller Air Cooled Chiller Industrial Chiller for Sale]

Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)]

Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3 Layer Round Tea Tin with Air Tight Lid, Part I.China PA System Power Amplifier with Enough Rated Power. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[13.56 MHz Fudan FM11RF08 Chip Card]

Export Grade Citric Acid Monohydrate as Detergent, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace.Familu Use Mini Small Pig Feed Pellet Mill, 175-184[Air Filter Box Type]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken]

Whitaker,J.R.(1963),3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light. Analytical Chemistry35(12), 1950-1953[Colombia Outdoor P5 Pantallas LED Display]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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