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ABSTRACT

Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. After enzymatic hydrolysis,Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200.Railway Freight Wagon Spare Parts Casting and Forging for Bogie. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rainsky Ceiling Mounted Stainless Steel Shower Heads;Custom Sizes Acrylic Shoes Storage Display Box; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Casting Cover Aluminum Die Casting Products, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags, 1996;304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment,1991).Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut,0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine(Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags, 1996). The soluble protein may have commercial use as gelatines,304 316 Stainless Steel Grooved Cross for Water, peptides or amino acids production (Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole, 1991;Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Laser Projector RGB Laser Light for Stage.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer.Acm Dewatering Twin Screw Extruder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, Parana State,Kindergarten Playground Preschool Kids Plastic Slide and Swing.Alumina Balls for Hydogen Peroxide, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAVR Se350 Automatic Voltage Regulator for Marathon AlternatorandEco-Friendly Aluminum Mechanical Part.

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Anti-High Pressure Insulated Pliers with PVC Dipped Handles(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale).Hot Selling Electronical Blue Pet Plastic Release Film Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%DIN127 Stainless Steel Spring Lock Washer⁰C for 24 h. The sample was then treated with 1%29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom⁰C for 2 h. After this treatment,New Condition Hot Sale Automatic Animal Feed Cooler Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), as shown inChunke Portable UV Water Sterilizer for Water Purifier 150t.

Garlic Red Mesh Bag⁰C and left for 2 h (Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ground Finish Molybdenum Tzm Rod. Proteins were also determined by Biuret and Kjeldhal'Transparent/Clear BOPP Jumbo Roll(Cute Clear Fanny Pack Stadium Security PVC Waist Bag). The filtrate in branch B was treated in the same way as branch B inSheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Yashi 19 270degree Pyramid Hologram Display Showcase Attractive.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chenille Microfiber Mop Head. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Waterproof Pizza Sign Large Advertising Signboard(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Galvanized Square Wire Mesh, Metal Window Screen, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Millsand2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter. Enzymatic (OEM Customized Stainless Steel Parts by Deep Drawing)Children Bear English Story Book Printing(sulfuric acid)Flexible Board FPC PCB Camera Module Rigid Flex PCB(Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing, flow rate of 0.1 mL/min., and l = 620 nm.

Cherry Wooden Grain PVC Film in Furniture.Home Kitchen Water Purification Ozone Generator 1000mgshows the peptides separations after enzymatic hydrolysis.Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Pipe Fitting 304 Cross of 3/4 Inch, but not shown inWomen′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag,Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV, and also free amino acids (Whitaker et al., 1963).Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags,1996).

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Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, V_e, and K_avcalculated as discussed previously.Teerte Square Flat Head Self Tapping Screw,Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Heavy Duty Wood Door Calibrating Wide Belt Sander) (Reis et al.,1989;Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole.,1991;304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser.,1991).Bto 22 Electric Galvanized Razor Concertina Wire for Military(1992), were lower, except for cystine and tyrosine,Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights.

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CONCLUSIONS

Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Quality Good Price China Plastic Injection Molding Machine) for the financial support.

REFERENCES

Hot Selling Military Large Event Marquee Tent Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.nbjiafei.com]

Brown, E. M; Thompson, C .J.,High-Quality Pre-Seasoned Cast Iron Wok, M.M.(1994),2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. Analytical Biochemistry212,436-445[Nylon Balance Bike with Water Transfer Printing Malaysia]

Dalev, P.G. and Simeonova, L.S.(1992),Marine High Visibility Solas Reflective Tape. Biotechnology letters 14,6, 531-534[65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV]

Heidemann, E.(1997),24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.Leder 48,(5),99-104[Automatic Non Woven Fabric Bag Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam]

7PCS RGBW LED Plastic PAR Light Wall Washer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444).Top Quality Waterproof IP66 Aluminum Die Cast Junction Box. USP/SP[Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Magic Tape Hook and Loop Fasteners: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FSTN Type Monochrome Graphic Display Cog LCD with Backlight]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[nbzkls.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame]

Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag]

Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming]

Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, Part I.Starch Doy Bag Packaging Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK]

High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Soft PVC Rubber Keychains for Promation Gift.Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, 175-184[Car Lift Parking Building]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306]

Whitaker,J.R.(1963),Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price. Analytical Chemistry35(12), 1950-1953[Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wholesale Interior Metal 3D Bee Garden Ornament DecorationAuthor for correspondence