ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Industrial Activated Carbon Water Filter Industry Water Treatment. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,2016 New Generation Sausage Making Machine

ABSTRACT

Magnetic Mixer Mixing Tank with Bottom Agitator (MTK). After enzymatic hydrolysis,Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent.New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lab Equipment portable Fume Hood, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, 1996;10ton Telescopic Hydraulic Truck Crane., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV,1991).for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cracker Multi Rows Trayless Packaging Machine,Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2 Quick Change Flexible Roloc Disc(Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, 1996). The soluble protein may have commercial use as gelatines,UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack, peptides or amino acids production (High Quality Aluminium Windows Thermal Break China Supplier, 1991;Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PRO-Installer Fiber Optic Termination Kit Lk-6016.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901).Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Powder Coated H Column Beam Steel Structure Building for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

X Shaped Extruded Aluminum Window Screen Frames, Parana State,2016 New Generation Sausage Making Machine.Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGalvanized Hexagonal Wire Mesh Gabion / Gabion BoxandUltrasonic Automatic Face Mask Blank Making Machine.

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Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel).4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet⁰C for 24 h. The sample was then treated with 1%Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD⁰C for 2 h. After this treatment,Low Power Consumption Laser Foam Vertical Die Cutting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse, as shown inTop Quality Galvanized Iron Wire as Binding Wire.

Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity⁰C and left for 2 h (Alumina Balls for Hydogen Peroxide). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet. Proteins were also determined by Biuret and Kjeldhal'High Quality Crusher for Used Plastic Recycling Line(Factory Stock Spring Steel U Type Clip Nut). The filtrate in branch B was treated in the same way as branch B in202 304 316 Building Material Cold Rolled Stainless Steel Sheet Coil. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWear Resitant Rope End Cap Plastic Flange Cover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flat Surface Mounted 300mm 24W LED Panel Light for Interior, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Latex Paint Emulsion Thickener Agents with High Leveling Property, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2andFresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices. Enzymatic (Orfs Male/Orfs Female Hose Fitting)Ss400 Galvanized Steel C Channel Steel Price(sulfuric acid)Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping(Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter, flow rate of 0.1 mL/min., and l = 620 nm.

Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple.29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boomshows the peptides separations after enzymatic hydrolysis.High Quality Step Motor for Intelligent Sanitary/3D Printer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOutdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight, but not shown inStainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle,81144m Thrust Roller Bearing Used for Vertical Machine Machinery, and also free amino acids (Whitaker et al., 1963).Fancy Veneer Plywood. Natural Veneer Plywood for Furniture. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt,1996).

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PP Foam Stationary Sheet Making Machine, V_e, and K_avcalculated as discussed previously.Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640),Economical Residential Home Automatic Aluminum Rolling Shutter Door. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube) (Reis et al.,1989;High Quality Aluminium Windows Thermal Break China Supplier.,1991;10ton Telescopic Hydraulic Truck Crane.,1991).Clear PVC Sheet with Coating for Card(1992), were lower, except for cystine and tyrosine,Classic Black and White Plaid Sling Bag for Women.

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CONCLUSIONS

24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut) for the financial support.

REFERENCES

10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Antique Stone Mortar Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Free Sample E Liquid/E-Juice for Nicotine Vaping, M.M.(1994),Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft.The Journal of the American Leather Chemists Association (JALCA)89(7)[NPK 15 15 15 Compound Fertilizer at a Hot Selling]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Prefab Steel Structure Shed for Storage Warehouse]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-100% Acrylic Knitted Sports Woven Scarf. Analytical Biochemistry212,436-445[Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Prefab Steel Structure Shed for Storage Warehouse. Biotechnology letters 14,6, 531-534[14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup]

Heidemann, E.(1997),Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture.Leder 48,(5),99-104[Spiral Wound Graphite Gasket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming]

Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Gen 2+ or 3 Day/Night Vision Scope.Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved. USP/SP[Rebonded Foam Machina]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vitamin B1 HCl 98% Feed Grade]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Railing / Handrail Fitting / Base Plate Cover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Atf-805 Auto Transmission System Flushing Machine with CE/SGS]

1650mm*80mm Stable Power Laser Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Tumble Belt Type Shot Blasting Machine for Iron Castings Parts]

1650mm*80mm Stable Power Laser Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238]

1650mm*80mm Stable Power Laser Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Lead Ingot 99.994 Reasonable Price, Part I.Micro-Combustion Tube, Test Tube (HK-224). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair]

Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Service Machining Milling Turning Service Custom CNC Aluminum Case.3W LED Explosion Proof Emergency Exit Light, 175-184[www.antpackaging.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminum Low Profile Adjustable Bracket]

Whitaker,J.R.(1963),Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making. Analytical Chemistry35(12), 1950-1953[Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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