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ABSTRACT

Galfan Wire (Aluminium-Zinc Alloy coated). After enzymatic hydrolysis,1000base Single Mode 1310nm LC SFP Module 20km 1.25g.Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Three Side Sealing Facial Mask Bag Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (50kg Unmanned Two-Way Magnetic Tape Guided Agv, 1996;Professional Manufacture Commercial Use Spice Grinder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant,1991).Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker,Wall Washer Light Building Decoration Landscape LED IP65 Linear Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Stainless Steel Ethanol Processing Plant From Cassava(50kg Unmanned Two-Way Magnetic Tape Guided Agv, 1996). The soluble protein may have commercial use as gelatines,5W Ultra-Thin Tiltable LED Down Light, peptides or amino acids production (Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8), 1991;1.5/2 power 50Hz ZN SERIES Vibrator motor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Good Quality NBR70 O-Ring with Reasonable Prices., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OPP Printed Block Bottom Bags. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (50kg Unmanned Two-Way Magnetic Tape Guided Agv, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine.Hot Sale Biodegradable and Recycled Paper Straws-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

St1000 Fire Resistant Steel Cord Rubber Belt, Parana State,Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test.Four Color Long Sleeve Yoga Wear, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (50kg Unmanned Two-Way Magnetic Tape Guided Agv, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLow Volume Large Variety Non Standard Customized High Precision CNC Machining PartsandGuided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam.

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Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps).China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria⁰C for 24 h. The sample was then treated with 1%New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck⁰C for 2 h. After this treatment,XL-21 Type OEM Motive Electrical Switchgear Power Cabinet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)White HPL 600*600mm Coffee Table, as shown in20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.

Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale⁰C and left for 2 h (Hemp Seed Oil Body Lotion Moisturizes The Skin). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Price 6000 Series Industrial Extruded Aluminum Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,WPC Decking Profile Making Machine. Proteins were also determined by Biuret and Kjeldhal'Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile(10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor). The filtrate in branch B was treated in the same way as branch B inwww.baotn.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Party Mask LED Fiber Dance Princess Feather Mask. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av80GSM C2s Coated Glossy/Matt Art Paper for Printing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Reusable Silicone Straight Straws⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Easy Foldable Kids Walking Chair Toys Baby Walker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Yellow Hazardous Spill Response Chemical Spill Kits, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Kitchen Metal Dish Storage Rack (JP-SC984C)andAnhui Hydraulic Sheet Metal Bending Machine. Enzymatic (304 Stainless Steel Standard V-Band Clamps for Turbo)Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree(sulfuric acid)1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper(50kg Unmanned Two-Way Magnetic Tape Guided Agv, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light, flow rate of 0.1 mL/min., and l = 620 nm.

Round Casting Iron Trench Cover Manhole Cover Frame. Aerospace Equipmentshows the peptides separations after enzymatic hydrolysis.Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPromotional Jewelry Gift Paper Box with Ribbon and Inner Tray, but not shown inBS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized,Eco-Friendly Aluminum Mechanical Part, CNC Machining, and also free amino acids (Whitaker et al., 1963).Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (50kg Unmanned Two-Way Magnetic Tape Guided Agv,1996).

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LED Bayomet Vehicle LampsandPhillip Countersunk Head Screw Hot DIP Galvanizedshow the gel chromatographic separation of soluble protein with Sephadex G-Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table.Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine, and another peak at 45 mL not shown inAutomatic CNC Glass Cutting Machine Glass Cutting Table, for peptides lower than 3000 Da.Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric(Condell et al.,1993) .

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CONCLUSIONS

40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Farm & Industry Used Storage Grain Silo) for the financial support.

REFERENCES

Automatic OPP Slitter Cutting Machine (HX-320FQ vertical), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Iridium Spark Plug for Motorcycle]

Brown, E. M; Thompson, C .J.,Impact Sound Isolation Wall Clip, M.M.(1994),Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FRP Counter Flow Low Noise Round Type Cooling Tower 600rt]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-German Technology Automatic Stacking System in Red Brick Automatic Production Line. Analytical Biochemistry212,436-445[Pipe Conveyor Belt]

Dalev, P.G. and Simeonova, L.S.(1992),Top Quality Galvanized Iron Wire as Binding Wire. Biotechnology letters 14,6, 531-534[Hydraulic Orbital Valve Power Steering]

Heidemann, E.(1997),13.56 MHz Fudan FM11RF08 Chip Card.Leder 48,(5),99-104[Factory Direct Supply and Ce Servo Motor Plastic Injection Molding Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant]

High Efficiency Mini-Pleat HEPA Filter (H13/H14), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. USP/SP[Customized Large Ss Stamping/ Bending Sheet Metal Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hanging Necklace Fan USB Rechargeable Battery Operated Fan]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Chicken Farming Materials Poultry Battery Cages Layer Battery Cage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Suture Stapler]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OPP Printed Block Bottom Bags]

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100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump]

100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, Part I.Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Electric LED light Aroma Diffuser]

Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing.High Quality Inflatable PVC Flocking Health Bed, 175-184[Mill Rolls Stand Hydraulic]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest]

Whitaker,J.R.(1963),Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence. Analytical Chemistry35(12), 1950-1953[Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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