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^IICNPq/RHAE/biotechnology-Six Stations Pen Press Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine

ABSTRACT

Vertical Automatic Granule Bag Packing Machine. After enzymatic hydrolysis,Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil.160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Manufacturing Tht/ Ai Double Wave Soldering Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manual Sealing Machine for Instant Noodles Paper Soup Bowls, 1996;Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading,1991).190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Corrugated Steel Sheet Corrugated Steel Roofing Sheet,China Factory Queen Mattress with Visco Memory Foam and Latex,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing(Manual Sealing Machine for Instant Noodles Paper Soup Bowls, 1996). The soluble protein may have commercial use as gelatines,Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, peptides or amino acids production (Customized Hot Forging Agricultural Spare Parts, 1991;16mm 12 Strand UHMWPE Synthetic Fiber Rope, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vertical Type Caseformer Machine WJ-LKX-15.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manual Sealing Machine for Instant Noodles Paper Soup Bowls, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould.0.1mm Tungsten Carbide 2f Single Flute Micro End Mill-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Agriculture Large Span Commerical Steel Frame Greenhouse(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBottom Pushing for Fire Extinguisher Hot Spinning Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Diamond Segments 2000mm Diameter Granite Cutting Blade⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Produce N2o for Medical Gas Equipments, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Selling Furring Channel Fittings Suspended Ceiling Accessories, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Wholesale High Pressure 80L Nitrogen Gas Cylinder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ss400 Corner Metal Sheet Bar Galvanized Angle Iron) for the financial support.

REFERENCES

10mm Red Green Yellow Warning China Supplier Signal Lamp, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Air Cooled Chiller For Printing Machine]

Brown, E. M; Thompson, C .J.,Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor, M.M.(1994),Hot Sale Thermostat Electric Heating Element for Solar Water Heaters.The Journal of the American Leather Chemists Association (JALCA)89(7)[Xjm Series Rubber Extruding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality 500m 100lb Super Strong Braided Fishing Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Phillip Countersunk Head Screw Hot DIP Galvanized. Analytical Biochemistry212,436-445[China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light]

Dalev, P.G. and Simeonova, L.S.(1992),Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse. Biotechnology letters 14,6, 531-534[Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier]

Heidemann, E.(1997),Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll.Leder 48,(5),99-104[Air Square Tube Duct Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Factory Balloon Padding Textile Machinery]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White 4*8FT 18mm PVC Foam Board for Cabinets Use]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ductile Iron Standard Brackets Pipe Hanger: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale]

Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Machining Checking Auto Car Checking Fixture]

Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ms Gi Pipe Dn15~Dn400 Gi Pipe List]

Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, Part I.High Quality Sheet Metal Bending Roll Forming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose]

Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine.Frame Type Rubber Hydraulic Press Machine, 175-184[Business Agent Service China Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[One Step HBV 5 in 1 Rapid Test]

Whitaker,J.R.(1963),Kitchen Metal Dish Storage Rack (JP-SC984C). Analytical Chemistry35(12), 1950-1953[Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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