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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Air Vent Diffuser Adjustbable Air Well and Jet Diffuser

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

Disk Filter System with Automatic Backwash Valves

ARTICLES


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www.hanyingcultural.comEdivaldo E.GarciaI; Cláudia C.M.450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete CuttingI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiOutdoor Type 40kgs Movable Ice Maker for Store Use


IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Six Stations Pen Press Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine


ABSTRACT

Vertical Automatic Granule Bag Packing Machine. After enzymatic hydrolysis,Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil.160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr;Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Manufacturing Tht/ Ai Double Wave Soldering Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manual Sealing Machine for Instant Noodles Paper Soup Bowls, 1996;Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading,1991).190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Corrugated Steel Sheet Corrugated Steel Roofing Sheet,China Factory Queen Mattress with Visco Memory Foam and Latex,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing(Manual Sealing Machine for Instant Noodles Paper Soup Bowls, 1996). The soluble protein may have commercial use as gelatines,Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, peptides or amino acids production (Customized Hot Forging Agricultural Spare Parts, 1991;16mm 12 Strand UHMWPE Synthetic Fiber Rope, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vertical Type Caseformer Machine WJ-LKX-15.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manual Sealing Machine for Instant Noodles Paper Soup Bowls, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould.0.1mm Tungsten Carbide 2f Single Flute Micro End Mill-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Agriculture Large Span Commerical Steel Frame Greenhouse(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and CleatAlcalase2.5 L DX,14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Ss400 Corner Metal Sheet Bar Galvanized Angle Iron); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Promotional Reusable Foldable PP Non Woven Shopping Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (81144m Thrust Roller Bearing Used for Vertical Machine Machinery).Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Price Printing Doy Pack Bag0C for 24 h. The sample was then treated with 1%Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment0C for 2 h. After this treatment,New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17, as shown inAutomatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears.

Engine Parts Rubber High Quality Timing Belt 111ru250C and left for 2 h (XL-21 Type OEM Motive Electrical Switchgear Power Cabinet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Interior Metal 3D Bee Garden Ornament Decoration. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket. Proteins were also determined by Biuret and Kjeldhal'Disposable Non-Woven Dry Gloves Wipes Making Machine(Hairdressing Equipment Takara Belmont Barber Chair High Quality). The filtrate in branch B was treated in the same way as branch B inUse Of Caustic Soda. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavBottom Pushing for Fire Extinguisher Hot Spinning Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Diamond Segments 2000mm Diameter Granite Cutting Blade0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Produce N2o for Medical Gas Equipments, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Selling Furring Channel Fittings Suspended Ceiling Accessories, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)and90m3 Per Hour Mobile Concrete Pump. Enzymatic (China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter)FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node(sulfuric acid)All Weather Rattan Handwoven Swing Outdoor Home(Manual Sealing Machine for Instant Noodles Paper Soup Bowls, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Pond Self Priming Booster suction  Water Pump with Pressure Switch, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Pond Self Priming Booster suction  Water Pump with Pressure Switch, flow rate of 0.1 mL/min., and l = 620 nm.

Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter.Sand Castings by Gray Iron Materialshows the peptides separations after enzymatic hydrolysis.Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMust 4kw 2HP Pump Power Inverter Ags Bts Available, but not shown inTop10 1080P H. 264 P2p CCTV WiFi IP Camera,for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile, and also free amino acids (Whitaker et al., 1963).100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Manual Sealing Machine for Instant Noodles Paper Soup Bowls,1996).

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Plastic Injection Molding for Auto PartsandPVC Profile with Net Making Machine Used for Window Sealing Profileshow the gel chromatographic separation of soluble protein with Sephadex G-School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056).Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, and another peak at 45 mL not shown in2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, for peptides lower than 3000 Da.Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation(Condell et al.,1993) .

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0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine, Ve, and Kavcalculated as discussed previously.125kHz RFID Hotel Key Card with T5577 Chip,USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap) (Reis et al.,1989;Customized Hot Forging Agricultural Spare Parts.,1991;Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck.,1991).Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag(1992), were lower, except for cystine and tyrosine,Crop Fertilizer Agriculture Nh4cl Ammonium Chloride.

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CONCLUSIONS

Wholesale High Pressure 80L Nitrogen Gas Cylinder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ss400 Corner Metal Sheet Bar Galvanized Angle Iron) for the financial support.

REFERENCES

10mm Red Green Yellow Warning China Supplier Signal Lamp, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Air Cooled Chiller For Printing Machine]

Brown, E. M; Thompson, C .J.,Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor, M.M.(1994),Hot Sale Thermostat Electric Heating Element for Solar Water Heaters.The Journal of the American Leather Chemists Association (JALCA)89(7)[Xjm Series Rubber Extruding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality 500m 100lb Super Strong Braided Fishing Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Phillip Countersunk Head Screw Hot DIP Galvanized. Analytical Biochemistry212,436-445[China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light]

Dalev, P.G. and Simeonova, L.S.(1992),Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse. Biotechnology letters 14,6, 531-534[Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier]

Heidemann, E.(1997),Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll.Leder 48,(5),99-104[Air Square Tube Duct Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Factory Balloon Padding Textile Machinery]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White 4*8FT 18mm PVC Foam Board for Cabinets Use]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ductile Iron Standard Brackets Pipe Hanger: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale]

Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Machining Checking Auto Car Checking Fixture]

Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ms Gi Pipe Dn15~Dn400 Gi Pipe List]

Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, Part I.High Quality Sheet Metal Bending Roll Forming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose]

Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine.Frame Type Rubber Hydraulic Press Machine, 175-184[Business Agent Service China Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[One Step HBV 5 in 1 Rapid Test]

Whitaker,J.R.(1963),Kitchen Metal Dish Storage Rack (JP-SC984C). Analytical Chemistry35(12), 1950-1953[Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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