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ABSTRACT

Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient. After enzymatic hydrolysis,White HPL 600*600mm Coffee Table.Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Price 6000 Series Industrial Extruded Aluminum Tube;WPC Decking Profile Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, 1996;Party Mask LED Fiber Dance Princess Feather Mask., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (80GSM C2s Coated Glossy/Matt Art Paper for Printing,1991).Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Reusable Silicone Straight Straws,Easy Foldable Kids Walking Chair Toys Baby Walker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Yellow Hazardous Spill Response Chemical Spill Kits(CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, 1996). The soluble protein may have commercial use as gelatines,Anhui Hydraulic Sheet Metal Bending Machine, peptides or amino acids production (304 Stainless Steel Standard V-Band Clamps for Turbo, 1991;Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Round Casting Iron Trench Cover Manhole Cover Frame. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane.Eco-Friendly Aluminum Mechanical Part, CNC Machining-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting TableAlcalase2.5 L DX,Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Ductile Iron Flanged Swing Check Valve, Parana State,XL-21 Type OEM Motive Electrical Switchgear Power Cabinet.Ozonated Water for Vegetables and Fruits Washing Ozone Generator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAdjustable Aluminum Solar Mounting SystemandStand 2.5t Electric Pallet Truck.

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Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)).Day and Night Zebra Blind Fabric Z1602(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock⁰C for 24 h. The sample was then treated with 1%Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)⁰C for 2 h. After this treatment,Impact Sound Isolation Wall Clip. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)German Technology Automatic Stacking System in Red Brick Automatic Production Line, as shown inPaper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon.

Top Quality Galvanized Iron Wire as Binding Wire⁰C and left for 2 h (Precision Engineering Parts Bearing Support Unit for Ball Screw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.13.56 MHz Fudan FM11RF08 Chip Card. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt. Proteins were also determined by Biuret and Kjeldhal'Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles(Discussion and Interpretation System). The filtrate in branch B was treated in the same way as branch B inSmart Small Concrete Pump. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSquare Premium Glass Perfume Bottle 100ml(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

UV Laser Marking Machine (with Air Cooling System)andHigh Quality Inflatable PVC Flocking Health Bed. Enzymatic (Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence)Cast Iron Butterfly Valve with Handles(sulfuric acid)Furniture Grade E1 Glue Plywood with Competitive Price(CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer, flow rate of 0.1 mL/min., and l = 620 nm.

Underground Garden 6W High Power Outdoor LED Lawn Light.12V Electric Portable Outdoor Water Fruit Car Fridge with Ceshows the peptides separations after enzymatic hydrolysis.Stamping Metal Parts Manufacturer Sheet Parts Fabrication(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inQueen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace, but not shown in100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree,Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag, and also free amino acids (Whitaker et al., 1963).3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories,1996).

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Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages, V_e, and K_avcalculated as discussed previously.Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration,Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Colorful PVC Foam Board with High Density and Customizable Size) (Reis et al.,1989;304 Stainless Steel Standard V-Band Clamps for Turbo.,1991;Party Mask LED Fiber Dance Princess Feather Mask.,1991).Stainless Steel Ozone Disinfection Machine Ozone Generator(1992), were lower, except for cystine and tyrosine,Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube.

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CONCLUSIONS

PVC Profile with Net Making Machine Used for Window Sealing Profile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric) for the financial support.

REFERENCES

Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Tank Longitudinal Seam Welding Manipulator for Pipe Welding Equipment]

Brown, E. M; Thompson, C .J.,Bobby Flay Websites Restaurant Martha Stewart Designer Tray, M.M.(1994),Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Personalized Coffee Mug Cup with Custom Logo]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber. Analytical Biochemistry212,436-445[Driving System for Hybrid Stepper Motor Dm860A]

Dalev, P.G. and Simeonova, L.S.(1992),Diameter 2.68m Paragraphed Autoclave for AAC Bricks. Biotechnology letters 14,6, 531-534[Small Backhoe Loader with Air Conditioning]

Heidemann, E.(1997),Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety.Leder 48,(5),99-104[Pecvd Coating Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass]

Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Travelling Weekender Mens Canvas Duffle Gym Sports Bag.4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box. USP/SP[4 Drawer Steel Office Vertical Filing Cabinet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Insecticidal Lamp with Solar Energy Frequent Vibration]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sinopec Sichuan Vinylon Works Vam Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel]

Best Selling Household Living Room Mat Chenille Carpets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),130HP 4WD Large Farm Power Engine Tractor From High Quality Factory-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sequins Butterfly Handmade Beads Embroidery Patch]

Best Selling Household Living Room Mat Chenille Carpets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory]

Best Selling Household Living Room Mat Chenille Carpets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment, Part I.Large Size Outdoor Store Front Signs Advertising Billboard. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[50mm Magnetic River Water Sewage Flow Meter for Acid]

Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1325 CNC Engraving Router.Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, 175-184[China Investment Casting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)]

Whitaker,J.R.(1963),T6s 86 PCD Core Bit for Geotechnical Drilling. Analytical Chemistry35(12), 1950-1953[Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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