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ABSTRACT

3D Embossed Iron on Label Clothing Heat Transfer Label. After enzymatic hydrolysis,Glass Food Jar & Plastic Lid.0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Building Material High Quality 316 Stainless Steel Grating, 1996;Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sc-2400 Automatic Multi Function Sosn Side Drilling Machine,1991).Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Quality Shaper Machine Bc6085 Shapping Machine,Thermoplastic Road Marking Paint Glass Beads,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine(Building Material High Quality 316 Stainless Steel Grating, 1996). The soluble protein may have commercial use as gelatines,Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, peptides or amino acids production (I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case, 1991;Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Free Sample! AC Power Contactor for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PA System Loudspeaker 100W Outdoor Column Speaker .

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Building Material High Quality 316 Stainless Steel Grating, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Breathable Surgical Disposable 3 Ply Earloop Face Mask.Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Medical Endoscope Disposable Bite Block with Bandage, Parana State,FSTN Type Monochrome Graphic Display Cog LCD with Backlight.Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Building Material High Quality 316 Stainless Steel Grating, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEco-Friendly Recycling PS Foam Container Production LineandMedical Packing Bags.

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Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man).Ladder Type Cable Tray with Ce/ GOST/ TUV/UL(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%12V Electric Portable Outdoor Water Fruit Car Fridge with Ce⁰C for 24 h. The sample was then treated with 1%Fine Bubble Diffuser for Air Purifier⁰C for 2 h. After this treatment,Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Cheap Price Custom Aluminum Logo Metal Label Plate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3120/17/38 Boltable Crane Rail Clips, as shown in1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine.

2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide⁰C and left for 2 h (Brake Disc). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Supplier Rubber Injection Molding Machine with Ce. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China. Proteins were also determined by Biuret and Kjeldhal'Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery(Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size). The filtrate in branch B was treated in the same way as branch B in10Ton Double Column Welding Positioner. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHonkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed 3000W Fiber Laser Cutter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Customized Aluminum Foil Shisha SheetandPersonality Cat Eye Sunglasses. Enzymatic (ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline)UV Coated PVC Panel for Interior Wall Board(sulfuric acid)Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.(Building Material High Quality 316 Stainless Steel Grating, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, flow rate of 0.1 mL/min., and l = 620 nm.

Wholesale 30W LED Down Lights with Budget Price.High Quality Mini LED Flood Light SMD Flood Light 50Wshows the peptides separations after enzymatic hydrolysis.Embossed Textured Wallpaper for Solvent Printing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, but not shown inAnti-High Pressure Insulated Pliers with PVC Dipped Handles,Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray, and also free amino acids (Whitaker et al., 1963).4-14 Manual Staple Gun with Staples for Hand Tools. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Building Material High Quality 316 Stainless Steel Grating,1996).

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High Speed Automatic Packing Machine (GK-650 BA), V_e, and K_avcalculated as discussed previously.5000-5900MHz 4G Built-in Automotive Antenna,Glass Lined Reactor for Chemical Factory. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla) (Reis et al.,1989;I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case.,1991;Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table.,1991).OEM Metal Stamping Stainless Steel Parts with CNC Machining(1992), were lower, except for cystine and tyrosine,Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper.

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CONCLUSIONS

Supermarket Plastic T-Wire Basket Swing up Price Label Holder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Design Indoor Artificial Mini Rockery Fountains) for the financial support.

REFERENCES

1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cnc Machine Manufacturing]

Brown, E. M; Thompson, C .J.,Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, M.M.(1994),Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[Steel Bar Peeling Lathe Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pipe Sheet Laser Cutter with Tube Cutting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Degradable PLA Plastic Bottle. Analytical Biochemistry212,436-445[Galfan Wire (Aluminium-Zinc Alloy coated)]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale China Merchandise 12V Horizontal Mini Camping Fridge. Biotechnology letters 14,6, 531-534[LED Screen Display Wireless Charging 10000mAh Power Bank]

Heidemann, E.(1997),20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House.Leder 48,(5),99-104[Hand Single-Shoulder Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Jewelry Ear Loops]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Parts for Crane Outrigger Hydraulic Oil Cylinder]

10ml Dropper Bottle for Skin Care Packing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom High Precision Micro Machining Service Brass Micromachining Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine]

10ml Dropper Bottle for Skin Care Packing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Car Seat Belt Buckle Mold Maker]

10ml Dropper Bottle for Skin Care Packing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag, Part I.Replacement Cell Phone LCD for iPhone 6s Spare Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Baby Feeding Chair Plastic Chair]

China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Explosion Proof Fog Cannon for Coal Plant.Silicone Large Non-Stick BPA Free Baking Mat Sheet, 175-184[Micro DC 24V Worm Gear Brushless Motor for Medical Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity]

Whitaker,J.R.(1963),19mic Black Cast PE Stretch Film for Pallet Wrapping. Analytical Chemistry35(12), 1950-1953[Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Food Grade Stainless Steel Threated Butterfly ValveAuthor for correspondence