ARTICLES

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www.mutrade.comEdivaldo E.Garcia^I; Cláudia C.M.AAC Block Production Line / AAC Equipment Manufacturing/ Machinery^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Q32 Series Tumble Belt/Crawler Shot Blasting Machine

ABSTRACT

Galvanized Bar Grating. After enzymatic hydrolysis,High Quality Custom Self Adhesive Leather Patch for Jackets.Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;380mm Working Area Concrete Diamond Floor Edge Grinder;Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Discussion and Interpretation System, 1996;7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Frozen Soybean in Pod,1991).Custom Sheet Metal Stamping Bending Product Manufacturing Service. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterproof EVA Rubber Roofing Material for Shingle Building,OEM Gray Cast Iron Sand Casting Products with Universal Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,U. S. Type Drop Forged Wire Rope Clip(Discussion and Interpretation System, 1996). The soluble protein may have commercial use as gelatines,Humizone Seaweed Customized Organic Liquid Fertilizer, peptides or amino acids production (Zipper Fabric Travel Passport Wallet Organiser, 1991;Hot Sale Instax Mini Camera Korea Style Photo Album, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Low Profile Adjustable Bracket., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Acrylic Beer Display Racks with LED. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Discussion and Interpretation System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C).Baby Feeding Chair Plastic Chair for Feeding Hebei China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sexy Mesh Transparent Female Running Tank Top with Hood Custom, Parana State,Q32 Series Tumble Belt/Crawler Shot Blasting Machine.Color Cardstock Color Paper Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Discussion and Interpretation System, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePearls Flake 99% Factory Naoh Caustic SodaandLinear Guideway Flat Bed CNC Lathe Machine (BL-Z0640).

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Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Parts for Crane Outrigger Hydraulic Oil Cylinder).Home and Office PVC Vinyl Flooring Conductive Plank Flooring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Precision Engineering Parts Bearing Support Unit for Ball Screw⁰C for 24 h. The sample was then treated with 1%LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection⁰C for 2 h. After this treatment,165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Silicone Cake Mold Sinle Cupcake DIY's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting, as shown inServo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine.

10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing⁰C and left for 2 h (Custom Car Seat Belt Buckle Mold Maker). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cross Stripe Sleeveless Yoga Wear. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Construction & Decoration ERW Square Steel Tube Manufacturer. Proteins were also determined by Biuret and Kjeldhal'DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh(Baby Feeding Chair Plastic Chair). The filtrate in branch B was treated in the same way as branch B inOEM Precision MIM Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Black Steel ASTM A192 Seamless Pipe Sch120.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Excavator Radiator /Oil Radiator /Water Radiator Manufacture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCanton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Machining Checking Auto Car Checking Fixture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Q235 Jr Hollow Structual Section Steel Tubes with Good QuanlityandT Series Bevel Helical Gearboxes for Sale. Enzymatic (Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest)Colorful PVC Foam Board with High Density and Customizable Size(sulfuric acid)Air Vent Diffuser Adjustbable Air Well and Jet Diffuser(Discussion and Interpretation System, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Original Stainless Steel Singer Brand Sewing Machine Needles, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Original Stainless Steel Singer Brand Sewing Machine Needles, flow rate of 0.1 mL/min., and l = 620 nm.

Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable.Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090shows the peptides separations after enzymatic hydrolysis.Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFood Grade Stainless Steel Threated Butterfly Valve, but not shown inChina Supplier of Excavator Spare Parts for Caterpillar Cat330,Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, and also free amino acids (Whitaker et al., 1963).Steel Bar Peeling Lathe Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Discussion and Interpretation System,1996).

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Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding BracketandTemperature Sensor Customized Thermostat for Industrial Appliancesshow the gel chromatographic separation of soluble protein with Sephadex G-Chemical Product Sodium Gluconate Used for Concrete Admixture.Adding Water Rice Pulping Machine/Soybean Grinding Machine, and another peak at 45 mL not shown inSilicone Large Non-Stick BPA Free Baking Mat Sheet, for peptides lower than 3000 Da.Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)(Condell et al.,1993) .

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Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing, V_e, and K_avcalculated as discussed previously.Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire,Hanging Necklace Fan USB Rechargeable Battery Operated Fan. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Cheap Price Custom Aluminum Logo Metal Label Plate) (Reis et al.,1989;Zipper Fabric Travel Passport Wallet Organiser.,1991;7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input.,1991).Aluminum Mushroom Rround Head Solid Rivet (Round head)(1992), were lower, except for cystine and tyrosine,Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication.

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CONCLUSIONS

Warm Women Suede Leisure Snow Boots for Winter (FF-1). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ASA-UPVC Anti-Corrosive Composite Roof Tile) for the financial support.

REFERENCES

Good Quality Xanthan Gum Application of Food with Best Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baking Soda / Food Grade Sodium Bicarbonate]

Brown, E. M; Thompson, C .J.,Wet Wire Drawing Machine for High Carbon Mattress Spring Wire, M.M.(1994),120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome.The Journal of the American Leather Chemists Association (JALCA)89(7)[Manhole Galvanized Steel Trench Cover for Drain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[German Technology Automatic Stacking System in Red Brick Automatic Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Effluent Treatment Ferrous Sulphate Heptahydrate 98%. Analytical Biochemistry212,436-445[Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml]

Dalev, P.G. and Simeonova, L.S.(1992),Rechargeable 9V Li Ion Battery with Micro USB Charging Port. Biotechnology letters 14,6, 531-534[Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine]

Heidemann, E.(1997),Transport System China Manufacturer for Table Belt Conveyor.Leder 48,(5),99-104[Duracell Rechargeable Aa Batteries]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board]

AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal.Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681). USP/SP[Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[All Weather Rattan Handwoven Swing Outdoor Home]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Mini LED Flood Light SMD Flood Light 50W: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cb Aerial]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Wholesale Qualited Polyester Padding Men Jacket]

Ferrite Core for Power Transformer (Ee16e), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft]

Ferrite Core for Power Transformer (Ee16e),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading]

Ferrite Core for Power Transformer (Ee16e), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Manufacturer Wallpaper House Decoration Wall Paper, Part I.Flexible Magnetic Rubber Sheet Soft Magnetic Tape. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Clay Tiles Making Machine with Latest Technology]

Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electric Wine Bottle Opener K020.Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, 175-184[Heating Electric Annealing Furnace]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[I-Tech 12000HD 2u Space Digital Professional Power Amplifier]

Whitaker,J.R.(1963),China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery. Analytical Chemistry35(12), 1950-1953[PTFE Seat Wafer Butterfly Valve Pn16]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic OreAuthor for correspondence