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www.clin-ele.comEdivaldo E.Garcia^I; Cláudia C.M.Hot Selling Military Large Event Marquee Tent Sale^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Chemical Plant Transfer Industrial Water Pump}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High-Quality Pre-Seasoned Cast Iron Wok. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle

ABSTRACT

Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. After enzymatic hydrolysis,Marine High Visibility Solas Reflective Tape.24L Thermoelectric Cooler and Warmer Car Mini Car Fridge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444);Top Quality Waterproof IP66 Aluminum Die Cast Junction Box; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Magic Tape Hook and Loop Fasteners, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, 1996;Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor,1991).Starch Doy Bag Packaging Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A),Fashion Soft PVC Rubber Keychains for Promation Gift,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper(Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, 1996). The soluble protein may have commercial use as gelatines,Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, peptides or amino acids production (Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes, 1991;Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Supply Cheap Custom Bleached White Cotton Gloves., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-304 &314 Stainless Steel Seamless Bending Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark.Custom Grey Full-Zip Cotton Mens Hoodies-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, Parana State,2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle.Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSMS 25g Non Woven Isolation Gown with Knitted Cuff for HospitalandBest Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector.

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Fcy30 Transport Mini Tipping Dumper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut).Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)⁰C for 24 h. The sample was then treated with 1%Premium Grade Rubber Cloth Duct Tape⁰C for 2 h. After this treatment,Sud1000h Poly Welder Butt Fusion Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Anti-Rust Torsion Spring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, as shown inStarch Doy Bag Packaging Machine.

New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt⁰C and left for 2 h (2 Quick Change Flexible Roloc Disc). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic. Proteins were also determined by Biuret and Kjeldhal'PP Woven Bag, Rice, Beans, Plastic Bag(Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration). The filtrate in branch B was treated in the same way as branch B inCMC Transparent Grade for Mining Production. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Professional Mold Factory Plastic Injection Mould Maker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Ice Cube Maker Ice Making Machine with Selectable Cube Size(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.24k Gold Whitening Anti-Spot Mask⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Clay Tiles Making Machine with Latest Technology, pH 3.25., mobile phase B: buffer solution of 0.01 mol/DIN Standard Gratings/Gavlanized Steel Grating, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Soldering Machine LED Module PCB Hot Air Soldering Stationand50mm Magnetic River Water Sewage Flow Meter for Acid. Enzymatic (PE Biodegradable Sheet Product Line/Single Screw Extruder)27L/Min Water Exchanger Heat Pump Mold Temperature Machine(sulfuric acid)Felt Belt for Textile Compactor(Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, flow rate of 0.1 mL/min., and l = 620 nm.

Metal Soft Enamel Souvenir Metal Medal Sport Medallions.Ground Finish Molybdenum Tzm Rodshows the peptides separations after enzymatic hydrolysis.20L Water Tank Lid Mold 3D Printing Samples Available(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in 20%Sc, but not shown inManufacturer of Soda Ash Dense 99 2% Price,Wholesale High Quality Metal Car Mount Holder Phone Holder, and also free amino acids (Whitaker et al., 1963).Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool,1996).

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New Design Multi-Color Silicone Coin Purse for Lady, V_e, and K_avcalculated as discussed previously.Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065,Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine) (Reis et al.,1989;Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes.,1991;Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010).,1991).Dark Green Plastic PVC Film for Christmas Tree Leaves(1992), were lower, except for cystine and tyrosine,Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor.

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CONCLUSIONS

Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys) for the financial support.

REFERENCES

1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Price Permanent Magnet Screw Air Compressor Copeland Compressor Screw Air Compressorpiston Air Compressor Atlas Copco VSD]

Brown, E. M; Thompson, C .J.,Water Treatment Anthracite, M.M.(1994),Heat Treated Welding Protection Glassfiber Cloth Ht800.The Journal of the American Leather Chemists Association (JALCA)89(7)[FTTX Solution Provider Fiber Optical 12cores MPO Cassette]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. Analytical Biochemistry212,436-445[Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved]

Dalev, P.G. and Simeonova, L.S.(1992),Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector. Biotechnology letters 14,6, 531-534[Hot Sale Instax Mini Camera Korea Style Photo Album]

Heidemann, E.(1997),Heat Pump Non-Programmable HVAC Room Thermostat.Leder 48,(5),99-104[Rock Wool Board for Building Wall as Fireproof 900X600X25mm]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sterile Dsiposable Syringe for Dental 12ml]

Hot Selling Military Large Event Marquee Tent Sale, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo.Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable. USP/SP[Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Rubber Nylon Conveyor Belting Automotive Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Double Heads Commercial Coffee Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PP Woven Bag]

Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Injector Pump Calibration Test Bench-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ASTM A240 321 Stainless Steel Sheet Price Per Kg]

Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ CNC Lathe Machine Milling]

Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ASME/ASTM Butt-Welding Stainless Steel Plate Flange, Part I.500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bending Sheet Metal Custom-Made Aluminum Bracket]

Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Polyester / Polypropylene Woven Geotextile for Road Construction.Granite Stone Jaw Crusher of Mining Machine, 175-184[Smt Inspection System]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pneumatic Air Driven Liquid Booster Pump]

Whitaker,J.R.(1963),Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor. Analytical Chemistry35(12), 1950-1953[Engine Parts Rubber High Quality Timing Belt 111ru25]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

White HPL 600*600mm Coffee TableAuthor for correspondence