ARTICLES

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ABSTRACT

Home Kitchen Water Purification Ozone Generator 1000mg. After enzymatic hydrolysis,PP Foam Stationary Sheet Making Machine.Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Economical Residential Home Automatic Aluminum Rolling Shutter Door;Clear PVC Sheet with Coating for Card; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Classic Black and White Plaid Sling Bag for Women, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, 1996;10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Free Sample E Liquid/E-Juice for Nicotine Vaping,1991).Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,100% Acrylic Knitted Sports Woven Scarf,Prefab Steel Structure Shed for Storage Warehouse,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture(24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, 1996). The soluble protein may have commercial use as gelatines,Gen 2+ or 3 Day/Night Vision Scope, peptides or amino acids production (Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, 1991;3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1650mm*80mm Stable Power Laser Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Lead Ingot 99.994 Reasonable Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Micro-Combustion Tube, Test Tube (HK-224).Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Service Machining Milling Turning Service Custom CNC Aluminum Case(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3PC Stainless Steel Flanged End Ball Valve 3 Inches 150(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output).Ysdcnc Manual Sheet Metal Plate Bending Roller Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Betaine Anhydrous 98% Food Grade Good Quality Competitive Price⁰C for 24 h. The sample was then treated with 1%2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover⁰C for 2 h. After this treatment,Bearing Stand Type Cam Follower. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Electric LED light Aroma Diffuser's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner, as shown inModern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale.

DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306⁰C and left for 2 h (PVC Ceiling Wall Pannelling). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,7PCS RGBW LED Plastic PAR Light Wall Washer. Proteins were also determined by Biuret and Kjeldhal'750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm(Furniture Grade E1 Glue Plywood with Competitive Price). The filtrate in branch B was treated in the same way as branch B inConcrete Polypropylene PP Waved Fibre. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Kindergarten Playground Preschool Kids Plastic Slide and Swing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rainsky Ceiling Mounted Stainless Steel Shower Heads⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Sizes Acrylic Shoes Storage Display Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Casting Cover Aluminum Die Casting Products, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Round Type Twin Screw Extruder Barrel ManufacturerandBakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags. Enzymatic (304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser)SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment(sulfuric acid)Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes(24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, flow rate of 0.1 mL/min., and l = 620 nm.

0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts.Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)shows the peptides separations after enzymatic hydrolysis.Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEmbossed Textured Wallpaper for Solvent Printing, but not shown inPowerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms,304 316 Stainless Steel Grooved Cross for Water, and also free amino acids (Whitaker et al., 1963).Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable,1996).

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Cashew Grading Machine for Cashew Processing Lineand Grooming Gloves Brushshow the gel chromatographic separation of soluble protein with Sephadex G-Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home.20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory, and another peak at 45 mL not shown inTravelling Weekender Mens Canvas Duffle Gym Sports Bag, for peptides lower than 3000 Da.Hot Sale Laser Projector RGB Laser Light for Stage(Condell et al.,1993) .

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CONCLUSIONS

Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stationery PP Clipboard With 7 Inch Scale Ruler) for the financial support.

REFERENCES

Good Quality Good Price China Plastic Injection Molding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.liweipvc.com]

Brown, E. M; Thompson, C .J.,Pet Hair Remover Glove for Dog, Grooming Gloves Brush, M.M.(1994),Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Supply Cheap Custom Bleached White Cotton Gloves]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Alumina Balls for Hydogen Peroxide. Analytical Biochemistry212,436-445[4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box]

Dalev, P.G. and Simeonova, L.S.(1992),Anti-High Pressure Insulated Pliers with PVC Dipped Handles. Biotechnology letters 14,6, 531-534[Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price]

Heidemann, E.(1997),Hot Selling Electronical Blue Pet Plastic Release Film Price.Leder 48,(5),99-104[Stainless Steel Gold Sliver Repair Jewelry Platinum Copper Laser Welding System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1.5 Inch High Pressure Petrol/Gasoline Water Pump]

Fancy Veneer Plywood. Natural Veneer Plywood for Furniture, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),DIN127 Stainless Steel Spring Lock Washer.29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom. USP/SP[Farm & Industry Used Storage Grain Silo]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Condition Hot Sale Automatic Animal Feed Cooler Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Perforated Pattern Aluminum Facade Panel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fish Scales Texture Fashion Leather for Shoe (S312100GH)]

Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Chicken Nipple Drinker System]

Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle]

Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Garlic Red Mesh Bag, Part I.Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling]

Ground Finish Molybdenum Tzm Rod, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Transparent/Clear BOPP Jumbo Roll.Yashi 19 270degree Pyramid Hologram Display Showcase Attractive, 175-184[High Lumen Aluminum Outdoor LED Wall Lamp 6W]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rectangular Stainless Steel Pipe SS304]

Whitaker,J.R.(1963),Chenille Microfiber Mop Head. Analytical Chemistry35(12), 1950-1953[Stainless Steel Ozone Disinfection Machine Ozone Generator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ISO 3821 3/16′′ Twin Line Welding Hose for USA MarketAuthor for correspondence