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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-16mm 12 Strand UHMWPE Synthetic Fiber Rope. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems


Vertical Type Caseformer Machine WJ-LKX-15. After enzymatic hydrolysis,Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine.Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;0.1mm Tungsten Carbide 2f Single Flute Micro End Mill;Agriculture Large Span Commerical Steel Frame Greenhouse; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, 1996;Ss400 Corner Metal Sheet Bar Galvanized Angle Iron., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine,1991).Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chinese Jbcz Handy Operation Threading Machine and Coupler,Promotional Reusable Foldable PP Non Woven Shopping Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board(14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, 1996). The soluble protein may have commercial use as gelatines,Factory Price Printing Doy Pack Bag, peptides or amino acids production (Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, 1991;New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Engine Parts Rubber High Quality Timing Belt 111ru25. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Wholesale Interior Metal 3D Bee Garden Ornament Decoration.Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Disposable Non-Woven Dry Gloves Wipes Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with VentingAlcalase2.5 L DX,Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Bottom Pushing for Fire Extinguisher Hot Spinning Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Fish Scales Texture Fashion Leather for Shoe (S312100GH)(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Diamond Segments 2000mm Diameter Granite Cutting Blade, Parana State,High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems.Produce N2o for Medical Gas Equipments, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years LifeandMedical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home.

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Hot Selling Furring Channel Fittings Suspended Ceiling Accessories(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box).90m3 Per Hour Mobile Concrete Pump(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter0C for 24 h. The sample was then treated with 1%FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node0C for 2 h. After this treatment,All Weather Rattan Handwoven Swing Outdoor Home. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pond Self Priming Booster suction  Water Pump with Pressure Switch's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter, as shown inEco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws.

Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace0C and left for 2 h (Heat Treated Welding Protection Glassfiber Cloth Ht800). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree. Proteins were also determined by Biuret and Kjeldhal'School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)(New Condition Hot Sale Automatic Animal Feed Cooler Machine). The filtrate in branch B was treated in the same way as branch B inCustomized Outdoor Inflatable Decoration Column with LED Light for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Crop Fertilizer Agriculture Nh4cl Ammonium Chloride, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Bio MediaandWholesale High Pressure 80L Nitrogen Gas Cylinder. Enzymatic (10mm Red Green Yellow Warning China Supplier Signal Lamp)Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor(sulfuric acid)Hot Sale Thermostat Electric Heating Element for Solar Water Heaters(14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Phillip Countersunk Head Screw Hot DIP Galvanized, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Phillip Countersunk Head Screw Hot DIP Galvanized, flow rate of 0.1 mL/min., and l = 620 nm.

Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse.28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayershows the peptides separations after enzymatic hydrolysis.Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndustrial Hydraulic Ice Grape Press Machine, but not shown inFlower Design Custom Made Silk Scarf,Heat Shrink Automatic Flow Wrapping Packing Machine, and also free amino acids (Whitaker et al., 1963).Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags,1996).

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Kitchen Metal Dish Storage Rack (JP-SC984C). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bottom Pushing for Fire Extinguisher Hot Spinning Machine) for the financial support.


0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pu Handbag Women Bag]

Brown, E. M; Thompson, C .J.,AVR Se350 Automatic Voltage Regulator for Marathon Alternator, M.M.(1994),Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom High Precision Micro Machining Service Brass Micromachining Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147. Analytical Biochemistry212,436-445[Automatic Three Side Sealing Facial Mask Bag Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High Strength Rigid PVC Plastic Foam Board for FRP. Biotechnology letters 14,6, 531-534[Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt]

Heidemann, E.(1997),Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building.Leder 48,(5),99-104[Off Road Trailer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Finished Tubing Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory]

LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench]

LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip]

LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, Part I.Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Glass Salt & Pepper Mill/Grinder Glass Spice Jar]

ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap.Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, 175-184[Tractor Hydraulic Steering Using 50-1000cc Steering Hydraulic Steering Control Unit]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[All Size Colored Tapping Hex Head Self Drill Screw Wood Screw]

Whitaker,J.R.(1963),High Quality Ceramic Chrome Hybrid Bearing Oil. Analytical Chemistry35(12), 1950-1953[Low Frequency 3000W 24V 230V Inverter with Battery Charger]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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