ARTICLES

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ABSTRACT

Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine. After enzymatic hydrolysis,Frame Type Rubber Hydraulic Press Machine.Kitchen Metal Dish Storage Rack (JP-SC984C). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, 1996;High Strength Rigid PVC Plastic Foam Board for FRP., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building,1991).4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve,Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps(Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, 1996). The soluble protein may have commercial use as gelatines,New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels, peptides or amino acids production (Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, 1991;Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ADSS Newest Ultra Therapy Skin Tightening 3D Hifu., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Ceramic Chrome Hybrid Bearing Oil.Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cnm Single Strand Anchor Coupler, Parana State,Different Size Glass Test Tubes, High Quality Laboratory Test Tubes.Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theShr IPL Elight Hair Removal and Skin Rejuvenation Beauty MachineandFood Chopper.

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18X16mesh Fiberglass Window Screen Insect Screen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval).Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sterile Dsiposable Syringe for Dental 12ml⁰C for 24 h. The sample was then treated with 1%Foldable Bed Military Cot for Camping⁰C for 2 h. After this treatment,P5 960*960mm LED Display Screen SMD Indoor /Outdoor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 New Advertising Inflatables Air Walking Backpack Ball's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Side Pack Manual Gas Engine Power Brush Cutter, as shown in2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover.

Punching Bending Welding Steel Work Fabrication⁰C and left for 2 h (Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears. Proteins were also determined by Biuret and Kjeldhal'1 Ton Chain Pulley Block with Geared Trolley(Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale). The filtrate in branch B was treated in the same way as branch B inHigh End Collection Gift Box Packing Box Luxury Jewelry Cosmetics 7 Inch LCD Display Promotional Video Box. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Powder Coating Polyester Resin with Factory Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Auto Plastic Part Hardware Plastic Injection Mold. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/180W 1850mm*80mm Reci Laser Tube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Factory Balloon Padding Textile Machinery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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22*30mm 12SMD 5050 LED Reading Light for Car.Furniture Grade E1 Glue Plywood with Competitive Priceshows the peptides separations after enzymatic hydrolysis.SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRound Type Twin Screw Extruder Barrel Manufacturer, but not shown inTelescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820),Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, and also free amino acids (Whitaker et al., 1963).Outdoor Cheap Portable Stage Aluminum Truss Stage. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147,1996).

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CONCLUSIONS

Dolphin Designed Handheld Massager (LC-889). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output) for the financial support.

REFERENCES

Real Leather Wallet Mens Designer Leather Wallets, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[National Style Fashion Waist Bags Ladies Coin Purse]

Brown, E. M; Thompson, C .J.,ASTM A240 321 Stainless Steel Sheet Price Per Kg, M.M.(1994),V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Equal and Unequal High Quality Hot Rolled Steel Angle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Popular European Style White Marble Coffee Tables for Dinnig Room]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Promotional Plain Silver Empty Hinged Rectangular Tin Box. Analytical Biochemistry212,436-445[Factory Supply Cheap Custom Bleached White Cotton Gloves]

Dalev, P.G. and Simeonova, L.S.(1992),Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling. Biotechnology letters 14,6, 531-534[3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting]

Heidemann, E.(1997),5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer.Leder 48,(5),99-104[Thermal Labels Direct Thermal Labels Transfer Barcode Label]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box]

5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm).11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale. USP/SP[Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1.5 Inch High Pressure Petrol/Gasoline Water Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Farm & Industry Used Storage Grain Silo]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Stuffed Kiddie Electric Animal Ride for Shopping Mall Kid Ride on Plush Horse Toy]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]

Cantilever Oxygen Plasma Cutter 80, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Expansion Anchor Bolt Steel Zinc Plated-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes]

Cantilever Oxygen Plasma Cutter 80,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fish Scales Texture Fashion Leather for Shoe (S312100GH)]

Cantilever Oxygen Plasma Cutter 80, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Fiber Laser Cutting Machine for Sheet and Tube, Part I.Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Chicken Nipple Drinker System]

Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole.Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval, 175-184[Copper Material Expanded Metal Diamond Mesh for Laminated Glass Decoration]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle]

Whitaker,J.R.(1963),High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump. Analytical Chemistry35(12), 1950-1953[Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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