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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Water Based Emulsion Paint Production Line. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Cnm Single Strand Anchor Coupler

ABSTRACT

Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp. After enzymatic hydrolysis,18X16mesh Fiberglass Window Screen Insect Screen.Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sterile Dsiposable Syringe for Dental 12ml;Foldable Bed Military Cot for Camping; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.P5 960*960mm LED Display Screen SMD Indoor /Outdoor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2019 New Advertising Inflatables Air Walking Backpack Ball, 1996;Side Pack Manual Gas Engine Power Brush Cutter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Punching Bending Welding Steel Work Fabrication,1991).Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears,1 Ton Chain Pulley Block with Geared Trolley,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Powder Coating Polyester Resin with Factory Price(2019 New Advertising Inflatables Air Walking Backpack Ball, 1996). The soluble protein may have commercial use as gelatines,Auto Plastic Part Hardware Plastic Injection Mold, peptides or amino acids production (Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine, 1991;IEC 62196-2 Type 2 EV Charging 32A Male Plug, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.180W 1850mm*80mm Reci Laser Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Factory Balloon Padding Textile Machinery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2019 New Advertising Inflatables Air Walking Backpack Ball, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy.Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, Parana State,Cnm Single Strand Anchor Coupler.Outdoor Cheap Portable Stage Aluminum Truss Stage, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2019 New Advertising Inflatables Air Walking Backpack Ball, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSanitary Stainless Steel Heating Cooling Mixing Buffer Storage TankandWide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04).

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One Arm Motorized Multifunction Hospital Equipment for China Supply(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Biodegradable and Recycled Paper Straws).Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display⁰C for 24 h. The sample was then treated with 1%Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine⁰C for 2 h. After this treatment,Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Printed Wristband Cotton Wristband RFID Woven Wristband, as shown inConstruction & Decoration ERW Square Steel Tube Manufacturer.

High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles⁰C and left for 2 h (M4X10 Round Head Core Pulling Aluminum Nail). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dolphin Designed Handheld Massager (LC-889). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Real Leather Wallet Mens Designer Leather Wallets. Proteins were also determined by Biuret and Kjeldhal'ASTM A240 321 Stainless Steel Sheet Price Per Kg(EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price). The filtrate in branch B was treated in the same way as branch B inDesigner Leather Handbags. The final soluble protein shown were used for peptides separations using gel permeation chromatography,V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Promotional Plain Silver Empty Hinged Rectangular Tin Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avExplosion-Proof Hydraulic Crawler Excavator with Quick Coupling(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Metal Window ScreenandCantilever Oxygen Plasma Cutter 80. Enzymatic (Stainless Steel Expansion Anchor Bolt Steel Zinc Plated)CNC Fiber Laser Cutting Machine for Sheet and Tube(sulfuric acid)Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets(2019 New Advertising Inflatables Air Walking Backpack Ball, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security, flow rate of 0.1 mL/min., and l = 620 nm.

Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole.High Quality Casting Cover Aluminum Die Casting Productsshows the peptides separations after enzymatic hydrolysis.Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine, but not shown inFood Grade Stainless Steel Milk Dairy Equipment for Factory,High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, and also free amino acids (Whitaker et al., 1963).10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2019 New Advertising Inflatables Air Walking Backpack Ball,1996).

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ID25mm Od86mm Standard Through Hole Slip Ring, V_e, and K_avcalculated as discussed previously.Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding,Bio Media. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter) (Reis et al.,1989;Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.,1991;Side Pack Manual Gas Engine Power Brush Cutter.,1991).Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine(1992), were lower, except for cystine and tyrosine,Platform Trolley with 150kg Capacity, 4 Castor Wheel.

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CONCLUSIONS

Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (22*30mm 12SMD 5050 LED Reading Light for Car) for the financial support.

REFERENCES

LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Industrial Footwear]

Brown, E. M; Thompson, C .J.,All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, M.M.(1994),Pre-Engineered Cage Broiler Poultry Farm Building.The Journal of the American Leather Chemists Association (JALCA)89(7)[9~12 Inch Mechanical Backing Plate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Alumina Ball with High Quality. Analytical Biochemistry212,436-445[7PCS RGBW LED Plastic PAR Light Wall Washer]

Dalev, P.G. and Simeonova, L.S.(1992),PVC Textile Fabric for Outdoor Sports Waterproof Backpack. Biotechnology letters 14,6, 531-534[Chicken Layer Cage Poultry Cage Chicken Cages]

Heidemann, E.(1997),White 4*8FT 18mm PVC Foam Board for Cabinets Use.Leder 48,(5),99-104[Glorystar Metal Steel Laser Cutting System for Tube and Pipe]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness]

Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating.Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants. USP/SP[ASA-UPVC Anti-Corrosive Composite Roof Tile]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sleeved Wire Harness for Industry Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Powerful Anti-Stress, Strong Flexibility Spoke Wire: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Promotional Reusable Foldable PP Non Woven Shopping Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[12ml Glass Oil Bottle with Dropper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PVC Cross Joint Pipe Fittings Equal Pipe Cross]

Modern American Style Beech Solid Wood Kitchen Cabinet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hero Brand Polypropylene Bags Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries]

Modern American Style Beech Solid Wood Kitchen Cabinet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers]

Modern American Style Beech Solid Wood Kitchen Cabinet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Machining Metal/Aluminum/Brass Auto Parts, Part I.Surprise! Range Hood Free! ! ! Juice Cart. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector]

Chicken Layer Cage Poultry Cage Chicken Cages, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),316L Cold Rolled Stainless Steel Coil 316L Inox.Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, 175-184[China Custom Swing Tags String Hang Tag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Modern American Style Beech Solid Wood Kitchen Cabinet]

Whitaker,J.R.(1963),Fully Automatic C Z Purlin Cold Roll Forming Machinery. Analytical Chemistry35(12), 1950-1953[8mm Heavy Duty Nails U Shaped Galvanized Staples]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle BlowerAuthor for correspondence