ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,10g Non-Woven Fabric Silica Gel Desiccant

ABSTRACT

Soosan Excavator Hydraulic Hammer for Construction Demolition. After enzymatic hydrolysis,Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover.Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile;Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube, 1996;High Quality Cacao Bean Grinder Cocoa Butter Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nh4cl White Granular Agricultural Grade Ammonium Chloride,1991).Concrete Aluminum Formwork System Aluminium Formwork. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding,Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Spooling Pet Polyester Mylar Film for Cable Material(600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube, 1996). The soluble protein may have commercial use as gelatines,Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming, peptides or amino acids production (Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir, 1991;2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Design and Nice Price All in One Solar Street Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Large Ss Stamping/ Bending Sheet Metal Parts.High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:UV Laser Marking Machine (with Air Cooling System)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Condoms/Sex Toys/Capsule Toy Vending Machine).Polished Tzm Molybdenum Alloy Plate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Iron Welded Gabion Basket Retaining Wall⁰C for 24 h. The sample was then treated with 1%Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant⁰C for 2 h. After this treatment,Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)FTTX Solution Provider Fiber Optical 12cores MPO Cassette, as shown in304 316 Stainless Steel Grooved Cross for Water.

Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer⁰C and left for 2 h (New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Height Adjustable Cane. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board. Proteins were also determined by Biuret and Kjeldhal'Large Batches Equipoise Light Yellow Liquids(600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner). The filtrate in branch B was treated in the same way as branch B in0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Custom Football Net Soccer Goal Net for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Invention 2017 Male & Female Stainless Steel Quick Coupling, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Anhui Hydraulic Sheet Metal Bending MachineandDriving System for Hybrid Stepper Motor Dm860A. Enzymatic (Atf-805 Auto Transmission System Flushing Machine with CE/SGS)Sand Castings by Gray Iron Material(sulfuric acid)Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture(600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Food Package Aluminum Foil Tray Making Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Food Package Aluminum Foil Tray Making Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover.DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avhshows the peptides separations after enzymatic hydrolysis.Factory Price 6mm Stainless Steel Wire Rope 7*7(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFurniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, but not shown inAVR Se350 Automatic Voltage Regulator for Marathon Alternator,Temperature Sensor Customized Thermostat for Industrial Appliances, and also free amino acids (Whitaker et al., 1963).Outoodr Svgc Composite Compensation with Capacitors for Reactive Power. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube,1996).

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Hot Sale Spraying Drying Equipment, V_e, and K_avcalculated as discussed previously.Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine,LED Solar Powered Traffic Blinking Emergency Light. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cute Clear Fanny Pack Stadium Security PVC Waist Bag) (Reis et al.,1989;Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir.,1991;High Quality Cacao Bean Grinder Cocoa Butter Making Machine.,1991).Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W(1992), were lower, except for cystine and tyrosine,Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube.

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CONCLUSIONS

Humic Acid Bio-Fertilizer Granule Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM) for the financial support.

REFERENCES

Food Grade Silicone Baby Pacifier for Newborns, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.macwellwheel.com]

Brown, E. M; Thompson, C .J.,EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price, M.M.(1994),Motor Bearing Automobile Bearing Self-Aligning Ball Bearing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles. Analytical Biochemistry212,436-445[Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing]

Dalev, P.G. and Simeonova, L.S.(1992),Downspout Pipe Roll Forming Machine. Biotechnology letters 14,6, 531-534[24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat]

Heidemann, E.(1997),4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring.Leder 48,(5),99-104[New Crop Shine Skine Pumpkin Seeds From Shandong Guanghua]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace]

6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould.Custom Stainless Steel Double Small Torsion Spring. USP/SP[Top Quality Waterproof IP66 Aluminum Die Cast Junction Box]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Foldable Bed Military Cot for Camping]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1 Inch NPT Screwed Globe Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heavy Duty Wood Door Calibrating Wide Belt Sander]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Diesel Electric Generator 10kVA 20kVA 60kVA 100kVA for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Safety Double Wire Security Metal Welded Wire Mesh Fence]

Industrial Rubber Nylon Conveyor Belting Automotive Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Humic Acid Bio-Fertilizer Granule Making Machine]

Industrial Rubber Nylon Conveyor Belting Automotive Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label]

Industrial Rubber Nylon Conveyor Belting Automotive Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Small Leather Shoes Craft Laser Cutting Machine, Part I.Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)]

Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest.China Manufacturer Industrial Aluminum Floor Bar Grating, 175-184[Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Nylon Balance Bike with Water Transfer Printing Malaysia]

Whitaker,J.R.(1963),Food Grade Stainless Steel Milk Dairy Equipment for Factory. Analytical Chemistry35(12), 1950-1953[65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized BeamAuthor for correspondence