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ABSTRACT

Original Stainless Steel Singer Brand Sewing Machine Needles. After enzymatic hydrolysis,Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable.Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Chemical Product Sodium Gluconate Used for Concrete Admixture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Adding Water Rice Pulping Machine/Soybean Grinding Machine, 1996;Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing,1991).Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hanging Necklace Fan USB Rechargeable Battery Operated Fan,Aluminum Mushroom Rround Head Solid Rivet (Round head),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication(Adding Water Rice Pulping Machine/Soybean Grinding Machine, 1996). The soluble protein may have commercial use as gelatines,Warm Women Suede Leisure Snow Boots for Winter (FF-1), peptides or amino acids production (Good Quality Xanthan Gum Application of Food with Best Price, 1991;Wet Wire Drawing Machine for High Carbon Mattress Spring Wire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Effluent Treatment Ferrous Sulphate Heptahydrate 98%.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rechargeable 9V Li Ion Battery with Micro USB Charging Port. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Adding Water Rice Pulping Machine/Soybean Grinding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Transport System China Manufacturer for Table Belt Conveyor.Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Flexible Magnetic Rubber Sheet Soft Magnetic Tape, Parana State,Air Vent Diffuser Adjustbable Air Well and Jet Diffuser.Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Adding Water Rice Pulping Machine/Soybean Grinding Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the LED Bayomet Vehicle LampsandPhillip Countersunk Head Screw Hot DIP Galvanized.

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Electric Wine Bottle Opener K020(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook).Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery⁰C for 24 h. The sample was then treated with 1%China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)⁰C for 2 h. After this treatment,Spine Shape Landscaping Artificial Turf Grass. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, as shown inAluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901).

Diesel Engine Type Zzs Sand Vibrating Screen⁰C and left for 2 h (Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Bluetooth Smart Watch with Heart Rate Monitor for Men 72087. Proteins were also determined by Biuret and Kjeldhal'Trendy High Quality 925 Stering Silver Tennis Glod Charm(18X16mesh Fiberglass Window Screen Insect Screen). The filtrate in branch B was treated in the same way as branch B inSnf/ Nsf/Pns/Fdn Superplasticizer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stand 2.5t Electric Pallet Truck. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull Body Neck Back Buttocks Vibrating Car Seat Massage Cushion(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Men Fashion Plaid Design Polyester Ties (WH24), pH 3.25., mobile phase B: buffer solution of 0.01 mol/70m Series Aluminiun Plastic Coated Profile for Widnows, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron ScrapandNew Arrival Wooden Kids Cleaning Set Toy for Education W10d251. Enzymatic (Vertical Type Automatic Box Erector Machine)Air Cooled Scroll Type Water Chiller for Industrial Process(sulfuric acid)Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China(Adding Water Rice Pulping Machine/Soybean Grinding Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, flow rate of 0.1 mL/min., and l = 620 nm.

Rotary Vibrating Sieve Machine for Compost.FRP Counter Flow Low Noise Round Type Cooling Tower 600rtshows the peptides separations after enzymatic hydrolysis.I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPipe Conveyor Belt, but not shown inHydraulic Orbital Valve Power Steering,Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, and also free amino acids (Whitaker et al., 1963).Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Adding Water Rice Pulping Machine/Soybean Grinding Machine,1996).

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Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), V_e, and K_avcalculated as discussed previously.Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price,Outdoor Type 40kgs Movable Ice Maker for Store Use. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle) (Reis et al.,1989;Good Quality Xanthan Gum Application of Food with Best Price.,1991;Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B).,1991).Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts(1992), were lower, except for cystine and tyrosine,Electric 100W Three Wheels Toy Car for Sale.

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CONCLUSIONS

2*2 4G Lte MIMO Antenna Rrouter/CPE. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel) for the financial support.

REFERENCES

2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Waw 8 Ton Logistics Light Truck for Transportation]

Brown, E. M; Thompson, C .J.,Electric Galvanized Coated Barbed Wire (MT-BW013), M.M.(1994),Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging.The Journal of the American Leather Chemists Association (JALCA)89(7)[Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Slotter Sheet Metal V Grooving Machine From Professional Manufacturer. Analytical Biochemistry212,436-445[ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill]

Dalev, P.G. and Simeonova, L.S.(1992),Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester. Biotechnology letters 14,6, 531-534[Auto Spare Parts 12629037 Ignition Coil for Cadillac]

Heidemann, E.(1997),Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool.Leder 48,(5),99-104[PVC Crust Foam Board Making Machine/ PVC Table Cloth Sheet Extrusion Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing]

Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter.China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor. USP/SP[St1000 Fire Resistant Steel Cord Rubber Belt]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cute Clear Fanny Pack Stadium Security PVC Waist Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Fitness EVA Yoga Mats]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket]

Personalized Coffee Mug Cup with Custom Logo, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Color Coated Aluminum Coils]

Personalized Coffee Mug Cup with Custom Logo,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Performance Multifunctional Crawler Mini Skid Steer Loader]

Personalized Coffee Mug Cup with Custom Logo, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:10 Pieces Customized Professional Makeup Brush, Part I.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts]

Electrophoresis Assembly Infilltration Tank for Effluent Treatment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),700mm RP HP UHP High Quality Graphite Electrode.Prefab Building Insulated Interior Concrete Partition Walls, 175-184[Military Relief Government Tents Large Outdoor Tents]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw]

Whitaker,J.R.(1963),Disk Filter System with Automatic Backwash Valves. Analytical Chemistry35(12), 1950-1953[Top China Forklift Supplier & Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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