ARTICLES

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ABSTRACT

Cross Stripe Sleeveless Yoga Wear. After enzymatic hydrolysis,Construction & Decoration ERW Square Steel Tube Manufacturer.DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Black Steel ASTM A192 Seamless Pipe Sch120;China Excavator Radiator /Oil Radiator /Water Radiator Manufacture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, 1996;Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Machining Checking Auto Car Checking Fixture,1991).Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,T Series Bevel Helical Gearboxes for Sale,Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Colorful PVC Foam Board with High Density and Customizable Size(BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, 1996). The soluble protein may have commercial use as gelatines,Air Vent Diffuser Adjustbable Air Well and Jet Diffuser, peptides or amino acids production (Original Stainless Steel Singer Brand Sewing Machine Needles, 1991;Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Steel Bar Peeling Lathe Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chemical Product Sodium Gluconate Used for Concrete Admixture.Adding Water Rice Pulping Machine/Soybean Grinding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, Parana State,10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing.Warm Women Suede Leisure Snow Boots for Winter (FF-1), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDIN Standard Gratings/Gavlanized Steel GratingandCoated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin.

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Good Quality Xanthan Gum Application of Food with Best Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing).Wet Wire Drawing Machine for High Carbon Mattress Spring Wire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome⁰C for 24 h. The sample was then treated with 1%Effluent Treatment Ferrous Sulphate Heptahydrate 98%⁰C for 2 h. After this treatment,Rechargeable 9V Li Ion Battery with Micro USB Charging Port. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Transport System China Manufacturer for Table Belt Conveyor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, as shown inCustomized Hot Forging Agricultural Spare Parts.

Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)⁰C and left for 2 h (Ladder Type Cable Tray with Ce/ GOST/ TUV/UL). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Mini LED Flood Light SMD Flood Light 50W. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ferrite Core for Power Transformer (Ee16e). Proteins were also determined by Biuret and Kjeldhal'Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel(Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair). The filtrate in branch B was treated in the same way as branch B inMagnetic Letters For Signs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Manufacturer Wallpaper House Decoration Wall Paper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flexible Magnetic Rubber Sheet Soft Magnetic Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing ModuleandSpine Shape Landscaping Artificial Turf Grass. Enzymatic (08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain)3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board(sulfuric acid)Diesel Engine Type Zzs Sand Vibrating Screen(BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply, flow rate of 0.1 mL/min., and l = 620 nm.

Bluetooth Smart Watch with Heart Rate Monitor for Men 72087.Printing Adhesive Waterproof Custom Membrane Switch for Electric Cookershows the peptides separations after enzymatic hydrolysis.Trendy High Quality 925 Stering Silver Tennis Glod Charm(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYsdcnc Manual Sheet Metal Plate Bending Roller Machine, but not shown in3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light,Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites, and also free amino acids (Whitaker et al., 1963).Stand 2.5t Electric Pallet Truck. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film,1996).

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Men Fashion Plaid Design Polyester Ties (WH24), V_e, and K_avcalculated as discussed previously.70m Series Aluminiun Plastic Coated Profile for Widnows,New Arrival Wooden Kids Cleaning Set Toy for Education W10d251. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ASME VIII Pressure Vessel for Curing Carbon Fiber Parts) (Reis et al.,1989;Original Stainless Steel Singer Brand Sewing Machine Needles.,1991;Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel.,1991).Vertical Type Automatic Box Erector Machine(1992), were lower, except for cystine and tyrosine,Air Cooled Scroll Type Water Chiller for Industrial Process.

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CONCLUSIONS

Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hanging Necklace Fan USB Rechargeable Battery Operated Fan) for the financial support.

REFERENCES

Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chemical Reactor with Heating and Mixing]

Brown, E. M; Thompson, C .J.,Rotary Vibrating Sieve Machine for Compost, M.M.(1994),I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud.The Journal of the American Leather Chemists Association (JALCA)89(7)[Latex Paint Emulsion Thickener Agents with High Leveling Property]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine. Analytical Biochemistry212,436-445[Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi]

Dalev, P.G. and Simeonova, L.S.(1992),Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. Biotechnology letters 14,6, 531-534[Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool]

Heidemann, E.(1997),Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet.Leder 48,(5),99-104[Naphtalene Based Admixtures Concrete Admixture Naphthalene Sulfonate Formaldehyde]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw]

Silicone Cake Mold Sinle Cupcake DIY, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube.Condoms/Sex Toys/Capsule Toy Vending Machine. USP/SP[Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Humizone Seaweed Customized Organic Liquid Fertilizer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Baby Finger Toothbrush]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.suchuangmachine.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Kindergarten Playground Preschool Kids Plastic Slide and Swing]

Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor Type 40kgs Movable Ice Maker for Store Use-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Blasting Cleaning Shot Peeling Machine Tumble Belt Type]

Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3D Embossed Iron on Label Clothing Heat Transfer Label]

Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, Part I.Electric 100W Three Wheels Toy Car for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED Solar Powered Traffic Blinking Emergency Light]

2*2 4G Lte MIMO Antenna Rrouter/CPE, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′).Electric Galvanized Coated Barbed Wire (MT-BW013), 175-184[Dc Spring Power Cable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Black Steel ASTM A192 Seamless Pipe Sch120]

Whitaker,J.R.(1963),Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging. Analytical Chemistry35(12), 1950-1953[Sexy Mesh Transparent Female Running Tank Top with Hood Custom]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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