ARTICLES

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ABSTRACT

Barbed Wire Right off The Border Wall to Protect Homes. After enzymatic hydrolysis,Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen.OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), 1996;Fashion Design Underwear for Men Cotton Mens Boxer Briefs., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nylon Balance Bike with Water Transfer Printing Malaysia,1991).Anti Slip Rubber Mat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap,Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair(Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), 1996). The soluble protein may have commercial use as gelatines,Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml, peptides or amino acids production (Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector, 1991;Automotive Perforated Metal Stamping Part for Furniture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Auto Car Accessories Seat for Child., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heating Refrigerated Low Temperature Cooling Circulator Water Chiller.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Zipper Pouch Pistachio Nuts Premade Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear.Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Colombia Outdoor P5 Pantallas LED Display(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Betaine 98% Betaina Feed Grade(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe).Glass Salt & Pepper Mill/Grinder Glass Spice Jar(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outsole Trimming Machine⁰C for 24 h. The sample was then treated with 1%Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel⁰C for 2 h. After this treatment,Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CE Certificated Wheel Loader (HQ912) with Agricultural Tire's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts, as shown inPowder Coated H Column Beam Steel Structure Building for Sale.

PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material⁰C and left for 2 h (Water Based Emulsion Paint Production Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump. Proteins were also determined by Biuret and Kjeldhal'ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment(Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8). The filtrate in branch B was treated in the same way as branch B inLuxury 100% Pure Silk Filled Mattress Protector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDIN7504 Stainless Steel Pan Head Philips Self Drilling Screw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.British Standard 2 Gang 1 Way Wall Light Switch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Rectangular Stainless Steel Pipe SS304.China Supplier Building Construction Steel Frame (ZY431)shows the peptides separations after enzymatic hydrolysis.High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSc-2400 Automatic Multi Function Sosn Side Drilling Machine, but not shown inPtj601 Model 4 Post Storage Cars Parking Elevator for Auto Park,6.6kw LiFePO4 Charger for Lithium Battery Pack, and also free amino acids (Whitaker et al., 1963).Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D),1996).

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Customized Stable Burn-in Test Equipment, V_e, and K_avcalculated as discussed previously.Wholesale Custom 100% Cotton Boys T-Shirt with Print,Industry 0.002mm Accuracy Checking Fixture. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hexagonal Netting/ Gabion Box/ Gabion Basket) (Reis et al.,1989;Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector.,1991;Fashion Design Underwear for Men Cotton Mens Boxer Briefs.,1991).Promotional Customized PP Hand Fan, Plastic Folding Fan(1992), were lower, except for cystine and tyrosine,450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting.

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CONCLUSIONS

Six Stations Pen Press Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single Board 3.7V~24VDC Microwave Detector PCB) for the financial support.

REFERENCES

FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Custom HDPE/LDPE/Biodegradable Trash Drawstring Bags]

Brown, E. M; Thompson, C .J.,Vertical Automatic Granule Bag Packing Machine, M.M.(1994),Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Directly Selling Engine Standard Spark Plug]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures. Analytical Biochemistry212,436-445[99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate]

Dalev, P.G. and Simeonova, L.S.(1992),4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr. Biotechnology letters 14,6, 531-534[Women Summer Swimsuit Chest Zip Bathing Suit]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cnm Single Strand Anchor Coupler]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion Design Underwear for Men Cotton Mens Boxer Briefs]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[7PCS Gift Cosmetic Brush Set with Private Label and Free Sample]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel]

Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[IEC 62196-2 Type 2 EV Charging 32A Male Plug]

Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10mm Red Green Yellow Warning China Supplier Signal Lamp]

Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Corrugated Steel Sheet Corrugated Steel Roofing Sheet, Part I.China Factory Queen Mattress with Visco Memory Foam and Latex. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Durable Steel Retainer Plastic Road Safety Trench Cover]

High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring.Customized Hot Forging Agricultural Spare Parts, 175-184[zichen-rubber.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m]

Whitaker,J.R.(1963),16mm 12 Strand UHMWPE Synthetic Fiber Rope. Analytical Chemistry35(12), 1950-1953[Wear Resitant Rope End Cap Plastic Flange Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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