ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Good Quality Tomato Sauce Making Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Vitamin B1 HCl 98% Feed Grade

ABSTRACT

2018 TPR Clean Teeth Training Dog Pet Chew Toy. After enzymatic hydrolysis,Good Quality Zinc Plated Slotted Hex Head Roofing Screw.Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof;Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Orthopedic Fiberglass Splint, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Amazon Hot Selling Kids Playing Pop up Goal, 1996;Small Frame Hydraulic Press Machine for Bending Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3.5-7.0g Ozone Generator Purification ---Commercial,1991).Baby Feeding Chair Plastic Chair. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Reflective Hand Hold Thermoplastic Road Marking,Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge(Amazon Hot Selling Kids Playing Pop up Goal, 1996). The soluble protein may have commercial use as gelatines,Food Grade Stainless Steel Threated Butterfly Valve, peptides or amino acids production (PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine, 1991;Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Suction Function Handheld Cool and Heat Food Bowl for Baby., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-JIS S Type Cast Iron Water Filter Dn25. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Amazon Hot Selling Kids Playing Pop up Goal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut.Factor Direct Adult Sex Toy Doll for Male Sex-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Poultry House, Livestock, Poultry Farm, Parana State,Vitamin B1 HCl 98% Feed Grade.1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Amazon Hot Selling Kids Playing Pop up Goal, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFood Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn BiodegradableandAluminum Foil Heat Shield Screen Matting Adhesive Backed.

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Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Stable Burn-in Test Equipment).Black Slim PU Leather Passport Travel Wallet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe⁰C for 24 h. The sample was then treated with 1%Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce⁰C for 2 h. After this treatment,3 Colors Tactical Shooting Hunting Camouflage Clothes Suit. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1.3KG Fire breaker for kitchen's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign, as shown inChina Manufacturer Wallpaper House Decoration Wall Paper.

Korean Integrated CTO Activated Carbon Filter for Water Treatment⁰C and left for 2 h (Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Vacuum Squeeze Filter Press for Coal Flotation Tailings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Modular Portable Shipping Container Lkit Building Home Villa House. Proteins were also determined by Biuret and Kjeldhal'New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel(China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall). The filtrate in branch B was treated in the same way as branch B inG80 Lifting Chain 6mm 8mm 10mm Diameter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Supplier of Excavator Spare Parts for Caterpillar Cat330.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAquarium Air Pump with 2 Nozzles Adjustable Gas Flow(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double-Offset Double Flanged Stainless Steel Butterfly Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automotive Acrylic Wholesale Masking Tape Factory Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Home Decoration Candle Glass Holder for Table Decorative, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVAandWholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars. Enzymatic (Wholesale Black Galvanized Head Stainless Steel Screw)Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle(sulfuric acid)Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair(Amazon Hot Selling Kids Playing Pop up Goal, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%OEM Logo Soft Silicone Reusable BPA Free Drinking Straws, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%OEM Logo Soft Silicone Reusable BPA Free Drinking Straws, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Worm Gear Fluorine Lined Butterfly Valve Flanged Type) for the financial support.

REFERENCES

Kwikstage Scaffolding System-Diagonal Brace, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.waterdroprofilter.com]

Brown, E. M; Thompson, C .J.,360 Rotating Automatic Touchless Car Wash Equipment with Drying System, M.M.(1994),48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner.The Journal of the American Leather Chemists Association (JALCA)89(7)[20kv 50kVA High Voltage Dry Type Power Supply Transformer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Enameled Mini Cast Iron Casserole]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox. Analytical Biochemistry212,436-445[Modular Portable Shipping Container Lkit Building Home Villa House]

Dalev, P.G. and Simeonova, L.S.(1992),IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump. Biotechnology letters 14,6, 531-534[Manual Vertical Car Key Cutting Machine for Locksmith]

Heidemann, E.(1997),Sell Rubber / EVA / PE/PVC Foam Board.Leder 48,(5),99-104[HVAC Aluminum Alloy Profile Ventilation Air Flow Ball Jet Diffuser]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Cacao Bean Grinder Cocoa Butter Making Machine]

Enameled Mini Cast Iron Casserole, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sodium Gluconate 98% Min Industry Grade for Chemical Admixture.Brake Disc. USP/SP[1650mm*80mm Stable Power Laser Tube]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hero Brand Polypropylene Bags Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Colorful Nylon Cable Ties]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bobby Flay Websites Restaurant Martha Stewart Designer Tray]

24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Export Grade Citric Acid Monohydrate as Detergent]

24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1325 CNC Engraving Router]

24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, Part I.LED Screen Display Wireless Charging 10000mAh Power Bank. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom LCD Screen Video Box for Package]

25kg 50kg Plastic PP Woven Feed Bags, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),360 Degree Rotation Medical Seat Cushion.Custom LCD Screen Video Box for Package, 175-184[Electric Car Body]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[50kg Unmanned Two-Way Magnetic Tape Guided Agv]

Whitaker,J.R.(1963),Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt. Analytical Chemistry35(12), 1950-1953[Boned Polyester Woven Faux Suede Sofa Fabric]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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