ARTICLES

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ABSTRACT

Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. After enzymatic hydrolysis,Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine.100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, 1996;Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Inflatable PVC Flocking Health Bed,1991).Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cast Iron Butterfly Valve with Handles,Furniture Grade E1 Glue Plywood with Competitive Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer(Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, 1996). The soluble protein may have commercial use as gelatines,Underground Garden 6W High Power Outdoor LED Lawn Light, peptides or amino acids production (Stamping Metal Parts Manufacturer Sheet Parts Fabrication, 1991;Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hexagonal Netting/ Gabion Box/ Gabion Basket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Different Thickness High Quality Aluminum Foil for Glass Bottle Lid. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla.Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor).Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Diameter 2.68m Paragraphed Autoclave for AAC Bricks⁰C for 24 h. The sample was then treated with 1%Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety⁰C for 2 h. After this treatment,Travelling Weekender Mens Canvas Duffle Gym Sports Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), as shown in300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction.

Best Selling Household Living Room Mat Chenille Carpets⁰C and left for 2 h (5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.130HP 4WD Large Farm Power Engine Tractor From High Quality Factory. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. Proteins were also determined by Biuret and Kjeldhal'Large Size Outdoor Store Front Signs Advertising Billboard(4 Styles Blue Glass Crystal Butt Beads Spiral Plug). The filtrate in branch B was treated in the same way as branch B inExperienced CNC Machining Parts for Aluminum CNC Milling Machining. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1325 CNC Engraving Router. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGarden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Machining 6601 Aluminum Parts with Anodized Automoblile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cummins L10 Engine Piston Cooling Nozzle 3070218, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Auto Spare Parts 12629037 Ignition Coil for Cadillac.Smallest Portable Waste Transformer Oil Purifier Machine Zy-6shows the peptides separations after enzymatic hydrolysis.High Quality PVC Resin with Low Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTransparent Color Stretch Wrap Film Manual Operate Wrap Film, but not shown inMotor Bearing Automobile Bearing Self-Aligning Ball Bearing,Small Backhoe Loader with Air Conditioning, and also free amino acids (Whitaker et al., 1963).Line Scan Uvss System Portable Under Vehicle Surveillance. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep,1996).

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Glass Food Jar & Plastic Lid, V_e, and K_avcalculated as discussed previously.0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer,Easy Operation Low Investment Cassava Silo. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper) (Reis et al.,1989;Stamping Metal Parts Manufacturer Sheet Parts Fabrication.,1991;Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing.,1991).CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making(1992), were lower, except for cystine and tyrosine,100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve.

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CONCLUSIONS

Building Material High Quality 316 Stainless Steel Grating. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube) for the financial support.

REFERENCES

Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5\\\\\\\" Truck Led Light]

Brown, E. M; Thompson, C .J.,Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, M.M.(1994),Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair.The Journal of the American Leather Chemists Association (JALCA)89(7)[PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quality Shaper Machine Bc6085 Shapping Machine. Analytical Biochemistry212,436-445[Automotive Acrylic Wholesale Masking Tape Factory Price]

Dalev, P.G. and Simeonova, L.S.(1992),Thermoplastic Road Marking Paint Glass Beads. Biotechnology letters 14,6, 531-534[Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light]

Heidemann, E.(1997),Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine.Leder 48,(5),99-104[Electric Scooter City Coco]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Trailer RV Vision Security]

Top Quality Galvanized Iron Wire as Binding Wire, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. USP/SP[Custom Waterproof Pizza Sign Large Advertising Signboard]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fine Bubble Diffuser for Air Purifier]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3240 Epoxy Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine]

Free Sample! AC Power Contactor for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PA System Loudspeaker 100W Outdoor Column Speaker -inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings]

Free Sample! AC Power Contactor for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Copper]

Free Sample! AC Power Contactor for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, Part I.Breathable Surgical Disposable 3 Ply Earloop Face Mask. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Customized Stainless Steel Parts by Deep Drawing]

Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw.Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, 175-184[Fiber Laser Cutting Machine Sheet Metal/CNC Fiber Laser Cutter Sheet Metal]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[One Arm Motorized Multifunction Hospital Equipment for China Supply]

Whitaker,J.R.(1963),Flower Design Custom Made Silk Scarf. Analytical Chemistry35(12), 1950-1953[Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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