ARTICLES

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ABSTRACT

4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall. After enzymatic hydrolysis,Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve.Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps;New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, 1996;ADSS Newest Ultra Therapy Skin Tightening 3D Hifu., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap,1991).Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Ceramic Chrome Hybrid Bearing Oil,Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder(Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, 1996). The soluble protein may have commercial use as gelatines,Digger Arm Small Wheel Loader (HQ908) with CE, peptides or amino acids production (15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer, 1991;Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Water Based Emulsion Paint Production Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cnm Single Strand Anchor Coupler.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰18X16mesh Fiberglass Window Screen Insect Screen.Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sterile Dsiposable Syringe for Dental 12ml(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Multipurpose Wireless PA Amplifier Lectern PA Speaker).1 Ton Chain Pulley Block with Geared Trolley(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Powder Coating Polyester Resin with Factory Price⁰C for 24 h. The sample was then treated with 1%Auto Plastic Part Hardware Plastic Injection Mold⁰C for 2 h. After this treatment,Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)IEC 62196-2 Type 2 EV Charging 32A Male Plug's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter, as shown inFDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Concert Stage Equipment Aluminum Stage Portable Stage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInvt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Cheap Portable Stage Aluminum Truss Stage, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery.Hot Sale Power Press Perforated Metal Punch Machineshows the peptides separations after enzymatic hydrolysis.Printed Wristband Cotton Wristband RFID Woven Wristband(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels, but not shown inGrade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt,High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles, and also free amino acids (Whitaker et al., 1963).Dolphin Designed Handheld Massager (LC-889). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module,1996).

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CONCLUSIONS

Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2019 New Advertising Inflatables Air Walking Backpack Ball) for the financial support.

REFERENCES

Cantilever Oxygen Plasma Cutter 80, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Very Hot Smart Portable Power Bank Battery Charger (EP266)]

Brown, E. M; Thompson, C .J.,Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, M.M.(1994),CNC Fiber Laser Cutting Machine for Sheet and Tube.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Degradable PLA Plastic Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets. Analytical Biochemistry212,436-445[Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion]

Dalev, P.G. and Simeonova, L.S.(1992),Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security. Biotechnology letters 14,6, 531-534[Nylon Cable Glands with CE]

Heidemann, E.(1997),Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole.Leder 48,(5),99-104[1zg-350 Single Side High Speed Rice Paddy Field Ridger Making Machine/Land Shaper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile]

Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval.High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump. USP/SP[Lab Equipment portable Fume Hood]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rechargeable 9V Li Ion Battery with Micro USB Charging Port]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lease Fitness Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle]

300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),BS 5163 Ductile Iron Rising Stem Gate Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Breathable Surgical Disposable 3 Ply Earloop Face Mask]

300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation]

300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Aluminum Sheet Press Brake, Part I.ID25mm Od86mm Standard Through Hole Slip Ring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light]

Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bio Media.Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, 175-184[www.sinoriseind.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)]

Whitaker,J.R.(1963),Platform Trolley with 150kg Capacity, 4 Castor Wheel. Analytical Chemistry35(12), 1950-1953[Hot Sale Ultra Slim Elevator Digital Signage]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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