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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Stamping Metal Parts Manufacturer Sheet Parts Fabrication. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag

ABSTRACT

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Key words;Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla;Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, 1996;Stainless Steel Ozone Disinfection Machine Ozone Generator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube,1991).PVC Profile with Net Making Machine Used for Window Sealing Profile. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch,Bobby Flay Websites Restaurant Martha Stewart Designer Tray,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line(Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, 1996). The soluble protein may have commercial use as gelatines,Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, peptides or amino acids production (Diameter 2.68m Paragraphed Autoclave for AAC Bricks, 1991;Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Travelling Weekender Mens Canvas Duffle Gym Sports Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Selling Household Living Room Mat Chenille Carpets.130HP 4WD Large Farm Power Engine Tractor From High Quality Factory-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CNC Machining 6601 Aluminum Parts with Anodized Automoblile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker).Cummins L10 Engine Piston Cooling Nozzle 3070218(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Round Shape All Color Pets Feeding Dog Bowl of China Supplies⁰C for 24 h. The sample was then treated with 1%Wvrb Series Planetary Gearbox Used for Robot⁰C for 2 h. After this treatment,NPK 15 15 15 Compound Fertilizer at a Hot Selling. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mini Blown Film Extrusion's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pearls Flake 99% Factory Naoh Caustic Soda, as shown inASME/ASTM Butt-Welding Stainless Steel Plate Flange.

Auto Spare Parts 12629037 Ignition Coil for Cadillac⁰C and left for 2 h (Transport System China Manufacturer for Table Belt Conveyor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality PVC Resin with Low Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Small Backhoe Loader with Air Conditioning. Proteins were also determined by Biuret and Kjeldhal'Line Scan Uvss System Portable Under Vehicle Surveillance(Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover). The filtrate in branch B was treated in the same way as branch B inm.hanyingcultural.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%FSTN Type Monochrome Graphic Display Cog LCD with Backlight. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3D Embossed Iron on Label Clothing Heat Transfer Label(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Easy Operation Low Investment Cassava Silo, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Quality Shaper Machine Bc6085 Shapping Machine.Fcy30 Transport Mini Tipping Dumpershows the peptides separations after enzymatic hydrolysis.Thermoplastic Road Marking Paint Glass Beads(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table, but not shown inThreaded Stainless Steel Motorized Electric Floating 3PC Ball Valve,Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, and also free amino acids (Whitaker et al., 1963).Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Square Plastic PP Geocomposite Drainage Blind Ditch/Drain,1996).

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CONCLUSIONS

Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1325 CNC Engraving Router) for the financial support.

REFERENCES

Flower Design Custom Made Silk Scarf, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bluetooth Led Wireless Speaker]

Brown, E. M; Thompson, C .J.,New Design Indoor Artificial Mini Rockery Fountains, M.M.(1994),7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Q32 Series Tumble Belt/Crawler Shot Blasting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Medical Endoscope Disposable Bite Block with Bandage. Analytical Biochemistry212,436-445[Plain Makeup Bag Canvas Cosmetic Bag]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels. Biotechnology letters 14,6, 531-534[7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane]

Heidemann, E.(1997),Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08).Leder 48,(5),99-104[Aluminum Foil Rewinding Machine (HAFA-850)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′)]

Underground Garden 6W High Power Outdoor LED Lawn Light, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ladder Type Cable Tray with Ce/ GOST/ TUV/UL.12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. USP/SP[Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fine Bubble Diffuser for Air Purifier: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Orthopedic Fiberglass Splint]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Slider Seat]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plain Chipboard]

Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Cheap Price Custom Aluminum Logo Metal Label Plate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories]

Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine]

Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3120/17/38 Boltable Crane Rail Clips, Part I.2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4]

China Supplier Rubber Injection Molding Machine with Ce, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China.Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, 175-184[7.5kw Variable Frequency Inverter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags]

Whitaker,J.R.(1963),High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free. Analytical Chemistry35(12), 1950-1953[Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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