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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-LED Solar Powered Traffic Blinking Emergency Light. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W

ABSTRACT

Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube. After enzymatic hydrolysis,Humic Acid Bio-Fertilizer Granule Making Machine.Food Grade Silicone Baby Pacifier for Newborns. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price;Motor Bearing Automobile Bearing Self-Aligning Ball Bearing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Downspout Pipe Roll Forming Machine, 1996;4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould,1991).Custom Stainless Steel Double Small Torsion Spring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1 Inch NPT Screwed Globe Valve,Industrial Rubber Nylon Conveyor Belting Automotive Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm(Downspout Pipe Roll Forming Machine, 1996). The soluble protein may have commercial use as gelatines,Small Leather Shoes Craft Laser Cutting Machine, peptides or amino acids production (Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop, 1991;Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Manufacturer Industrial Aluminum Floor Bar Grating.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Food Grade Stainless Steel Milk Dairy Equipment for Factory. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Downspout Pipe Roll Forming Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine.Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Low Frequency 3000W 24V 230V Inverter with Battery Charger(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

200W/300W Die Casting Aluminum LED Profile Spot Effect Light, Parana State,Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W.2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Downspout Pipe Roll Forming Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAuto Car Accessories Seat for ChildandCustom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service.

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Ce Approved Sperm Concentration Test for Sperm Density Test(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yashi 19 270degree Pyramid Hologram Display Showcase Attractive).Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation⁰C for 24 h. The sample was then treated with 1%Sand Casting Parts Large Size for Industrial Use⁰C for 2 h. After this treatment,Durable Steel Retainer Plastic Road Safety Trench Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Iron Cast Brake Drums for Trucks's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws, as shown inPortable Electric Concrete Vibrator with 18000 High Rotation Speed.

Folding Aluminium Easy Move Choral Stage with Wheels for School⁰C and left for 2 h (Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing. Proteins were also determined by Biuret and Kjeldhal'Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor(China Supplier Rubber Injection Molding Machine with Ce). The filtrate in branch B was treated in the same way as branch B inAluminum Profile Tube. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Computerized Single Fishnet Jacquard Circular Knitting Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMilk Cream Disc Centrifugal Separator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Counter Top Working Table Chiller, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cashew Grading Machine for Cashew Processing Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Custom High Precision Micro Machining Service Brass Micromachining Partsand4X4 Wd Car Tail Light for Ranger 2012-2016. Enzymatic (Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable)New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings(sulfuric acid)Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(Downspout Pipe Roll Forming Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot Sale Power Press Perforated Metal Punch Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot Sale Power Press Perforated Metal Punch Machine, flow rate of 0.1 mL/min., and l = 620 nm.

100%Polyester Satin Fabric for Blouse.Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Closeshows the peptides separations after enzymatic hydrolysis.Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Three Side Sealing Facial Mask Bag Making Machine, but not shown inYutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt,Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools, and also free amino acids (Whitaker et al., 1963).High-End Compression Rash Guards, Long Sleeve Compression Shirt. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Downspout Pipe Roll Forming Machine,1996).

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9~12 Inch Mechanical Backing Plate, V_e, and K_avcalculated as discussed previously.DIN929 Low-Cost Supply Hexagon Weld Nut,Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench) (Reis et al.,1989;Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop.,1991;4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring.,1991).High Efficiency Solar Cell Photovoltaic 300W Hot Sell(1992), were lower, except for cystine and tyrosine,60m3/H Lamella Clarifier for River Water Treatment.

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CONCLUSIONS

SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (8mm Heavy Duty Nails U Shaped Galvanized Staples) for the financial support.

REFERENCES

Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fiber Optic Cleaning Kit High Quality Supplier]

Brown, E. M; Thompson, C .J.,High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes, M.M.(1994),Soldering Machine LED Module PCB Hot Air Soldering Station.The Journal of the American Leather Chemists Association (JALCA)89(7)[All Size Colored Tapping Hex Head Self Drill Screw Wood Screw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Low Frequency 3000W 24V 230V Inverter with Battery Charger]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist. Analytical Biochemistry212,436-445[Ss400 Galvanized Steel C Channel Steel Price]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W. Biotechnology letters 14,6, 531-534[School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)]

Heidemann, E.(1997),High Safety Double Wire Security Metal Welded Wire Mesh Fence.Leder 48,(5),99-104[Fuel Injector Nozzle Ce6479-S1 for High Quality]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera]

Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pneumatic Air Driven Liquid Booster Pump.Heavy Duty Wood Door Calibrating Wide Belt Sander. USP/SP[Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Mobile Smartphone for Xiaomi 9 PRO]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bamboo Tea Cup]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker]

Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),X-Humate 95% Min Super Sodium Humate (Nut Moradant)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud]

Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting]

Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, Part I.Women Summer Swimsuit Chest Zip Bathing Suit. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint]

Hemp Seed Oil Body Lotion Moisturizes The Skin, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh.Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal, 175-184[12.7X0.7 mm Tp316L Stainless Steel Tube Manufacturers Welded Steel Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PVC Shrink Sleeve Label for Drinking Water Bottle]

Whitaker,J.R.(1963),UTP CAT6 48 Ports Vertical Termination Patch Panel. Analytical Chemistry35(12), 1950-1953[750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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