ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Premium Grade Rubber Cloth Duct Tape

ABSTRACT

Sud1000h Poly Welder Butt Fusion Machine. After enzymatic hydrolysis,Customized Anti-Rust Torsion Spring.Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PP Woven Bag, Rice, Beans, Plastic Bag, 1996;China Professional Mold Factory Plastic Injection Mould Maker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated,1991).Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Industrial Carbon Steel Wire Hose Hydraulic Pipe,24k Gold Whitening Anti-Spot Mask,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Clay Tiles Making Machine with Latest Technology(PP Woven Bag, Rice, Beans, Plastic Bag, 1996). The soluble protein may have commercial use as gelatines,DIN Standard Gratings/Gavlanized Steel Grating, peptides or amino acids production (50mm Magnetic River Water Sewage Flow Meter for Acid, 1991;PE Biodegradable Sheet Product Line/Single Screw Extruder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;27L/Min Water Exchanger Heat Pump Mold Temperature Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Felt Belt for Textile Compactor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PP Woven Bag, Rice, Beans, Plastic Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Metal Soft Enamel Souvenir Metal Medal Sport Medallions.20L Water Tank Lid Mold 3D Printing Samples Available-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale High Quality Metal Car Mount Holder Phone Holder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, MasterbatchAlcalase2.5 L DX,High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Pet Laminate Aluminium Foil/Pet+Alu+PE); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

New Design Multi-Color Silicone Coin Purse for Lady, Parana State,Premium Grade Rubber Cloth Duct Tape.Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PP Woven Bag, Rice, Beans, Plastic Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the100-350mm Butt Welding Angle Ammonia Freon Stop and Check ValveandChina Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery.

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Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (marble Makeup Brush Set for Travel in 20PCS).Dark Green Plastic PVC Film for Christmas Tree Leaves(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor⁰C for 24 h. The sample was then treated with 1%Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)⁰C for 2 h. After this treatment,1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Water Treatment Anthracite's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Heat Treated Welding Protection Glassfiber Cloth Ht800, as shown inRound Casting Iron Trench Cover Manhole Cover Frame.

Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring⁰C and left for 2 h (iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Heat Pump Non-Programmable HVAC Room Thermostat. Proteins were also determined by Biuret and Kjeldhal'Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo(Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board). The filtrate in branch B was treated in the same way as branch B in1ml Pharmaceutical Injection Glass Ampoule with Color Point. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Injector Pump Calibration Test Bench(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ASME/ASTM Butt-Welding Stainless Steel Plate Flange⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Alu 2319 Slimline 12mm Wide Recessed ProfileandPolyester / Polypropylene Woven Geotextile for Road Construction. Enzymatic (Granite Stone Jaw Crusher of Mining Machine)Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor(sulfuric acid)Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer(PP Woven Bag, Rice, Beans, Plastic Bag, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag.Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africashows the peptides separations after enzymatic hydrolysis.Wholesale All Kinds Cheap Plush Animal Baby Slippers(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAAC Block Production Line / AAC Equipment Manufacturing/ Machinery, but not shown inNew Design Indoor Artificial Mini Rockery Fountains,Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car, and also free amino acids (Whitaker et al., 1963).Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PP Woven Bag, Rice, Beans, Plastic Bag,1996).

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Food Kitchen Mini Milling Electric Grinding Maize and Flour Making MachineandRestaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in Chinashow the gel chromatographic separation of soluble protein with Sephadex G-Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine.New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress, and another peak at 45 mL not shown inSs400 Corner Metal Sheet Bar Galvanized Angle Iron, for peptides lower than 3000 Da.Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale(Condell et al.,1993) .

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Taiding Custom Tank Sheet Metal Fabrication, V_e, and K_avcalculated as discussed previously.Airtight Candy Bin with Scoop,Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse) (Reis et al.,1989;50mm Magnetic River Water Sewage Flow Meter for Acid.,1991;China Professional Mold Factory Plastic Injection Mould Maker.,1991).Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell(1992), were lower, except for cystine and tyrosine,3 Layer Round Tea Tin with Air Tight Lid.

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CONCLUSIONS

China PA System Power Amplifier with Enough Rated Power. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pet Laminate Aluminium Foil/Pet+Alu+PE) for the financial support.

REFERENCES

Export Grade Citric Acid Monohydrate as Detergent, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[RFID Cards RFID Silicone Wristbands RFID Key Tags with Personalization]

Brown, E. M; Thompson, C .J.,Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace, M.M.(1994),Familu Use Mini Small Pig Feed Pellet Mill.The Journal of the American Leather Chemists Association (JALCA)89(7)[Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Aluminium Windows Thermal Break China Supplier]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light. Analytical Biochemistry212,436-445[Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker]

Dalev, P.G. and Simeonova, L.S.(1992),Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer. Biotechnology letters 14,6, 531-534[Super Fireproof Antibiosis Household Spc Vinyl Floor Plank]

Heidemann, E.(1997),Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed.Leder 48,(5),99-104[Badminton Court Flooring]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122]

Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hairdressing Equipment Takara Belmont Barber Chair High Quality.Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000). USP/SP[Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Buy Single Item Moving Bee Eye Light Lowly Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[DIN Standard Gratings/Gavlanized Steel Grating]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[17 Languages Waterproof IP65 Biometric Network Fingerprint Access Control with ID Card]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin]

High Quality Customized Aluminum Foil Shisha Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[En1092-1 05 Pn16 Blind Flat Face Flange]

High Quality Customized Aluminum Foil Shisha Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing]

High Quality Customized Aluminum Foil Shisha Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment, Part I.Rectangular Box Facial Tissue Paper Fk-110. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Hot Forging Agricultural Spare Parts]

SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door.AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, 175-184[Custom 6 Panels Sport Cap Cotton Winter Snapback Camo Hat Fashion Visor Baseball Cap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ladder Type Cable Tray with Ce/ GOST/ TUV/UL]

Whitaker,J.R.(1963),CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine. Analytical Chemistry35(12), 1950-1953[Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing ModuleAuthor for correspondence