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ABSTRACT

Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting. After enzymatic hydrolysis,10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing.Cross Stripe Sleeveless Yoga Wear. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Construction & Decoration ERW Square Steel Tube Manufacturer;DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Black Steel ASTM A192 Seamless Pipe Sch120, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, 1996;Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film,1991).Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Machining Checking Auto Car Checking Fixture,Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,T Series Bevel Helical Gearboxes for Sale(China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, 1996). The soluble protein may have commercial use as gelatines,Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, peptides or amino acids production (Colorful PVC Foam Board with High Density and Customizable Size, 1991;Air Vent Diffuser Adjustbable Air Well and Jet Diffuser, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Original Stainless Steel Singer Brand Sewing Machine Needles., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve.Steel Bar Peeling Lathe Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chemical Product Sodium Gluconate Used for Concrete Admixture(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hanging Necklace Fan USB Rechargeable Battery Operated Fan, Parana State,Silicone Cake Mold Sinle Cupcake DIY.Aluminum Mushroom Rround Head Solid Rivet (Round head), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSoldering Machine LED Module PCB Hot Air Soldering StationandGround Finish Molybdenum Tzm Rod.

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Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Manufacturer of Soda Ash Dense 99 2% Price).Warm Women Suede Leisure Snow Boots for Winter (FF-1)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Good Quality Xanthan Gum Application of Food with Best Price⁰C for 24 h. The sample was then treated with 1%Wet Wire Drawing Machine for High Carbon Mattress Spring Wire⁰C for 2 h. After this treatment,120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Effluent Treatment Ferrous Sulphate Heptahydrate 98%'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rechargeable 9V Li Ion Battery with Micro USB Charging Port, as shown inBarbed Wire Right off The Border Wall to Protect Homes.

Transport System China Manufacturer for Table Belt Conveyor⁰C and left for 2 h (New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681). Proteins were also determined by Biuret and Kjeldhal'High Quality Mini LED Flood Light SMD Flood Light 50W(Magnifying Mirror Shaving Mirror 2X). The filtrate in branch B was treated in the same way as branch B inPortable Solar Panel 160W for Camping with Motorhome. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ferrite Core for Power Transformer (Ee16e).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Manufacturer Wallpaper House Decoration Wall Paper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Wine Bottle Opener K020, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide PowderandChina Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery. Enzymatic (China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010))Spine Shape Landscaping Artificial Turf Grass(sulfuric acid)08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain(China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, flow rate of 0.1 mL/min., and l = 620 nm.

Diesel Engine Type Zzs Sand Vibrating Screen.Outdoor Concert Stage Equipment Aluminum Stage Portable Stageshows the peptides separations after enzymatic hydrolysis.Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine, but not shown inCe 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery,Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, and also free amino acids (Whitaker et al., 1963).Trendy High Quality 925 Stering Silver Tennis Glod Charm. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Excavator Radiator /Oil Radiator /Water Radiator Manufacture,1996).

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CONCLUSIONS

Vertical Type Automatic Box Erector Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing) for the financial support.

REFERENCES

Air Cooled Scroll Type Water Chiller for Industrial Process, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sodium Bicarbonate 99% Promotion Hot]

Brown, E. M; Thompson, C .J.,Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China, M.M.(1994),Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090.The Journal of the American Leather Chemists Association (JALCA)89(7)[Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PP Woven Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rotary Vibrating Sieve Machine for Compost. Analytical Biochemistry212,436-445[ASTM A240 321 Stainless Steel Sheet Price Per Kg]

Dalev, P.G. and Simeonova, L.S.(1992),I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud. Biotechnology letters 14,6, 531-534[ CNC Lathe Machine Milling]

Heidemann, E.(1997),Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine.Leder 48,(5),99-104[Brushless Dc Submersible Solar Pumps Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bending Sheet Metal Custom-Made Aluminum Bracket]

LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa.Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet. USP/SP[Pneumatic Air Driven Liquid Booster Pump]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Engine Parts Rubber High Quality Timing Belt 111ru25]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[White HPL 600*600mm Coffee Table]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[40 FT 3 Axle Heavy Duty Semi Trailer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant]

Condoms/Sex Toys/Capsule Toy Vending Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Air Cooled Scroll Type Water Chiller for Industrial Process]

Condoms/Sex Toys/Capsule Toy Vending Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mini Blown Film Extrusion]

Condoms/Sex Toys/Capsule Toy Vending Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price, Part I.Outdoor Type 40kgs Movable Ice Maker for Store Use. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured]

Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electric 100W Three Wheels Toy Car for Sale.2*2 4G Lte MIMO Antenna Rrouter/CPE, 175-184[Anti-Mud Agent For Concrete]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer]

Whitaker,J.R.(1963),2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′). Analytical Chemistry35(12), 1950-1953[Rectangular Box Facial Tissue Paper Fk-110]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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