ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Ductile Iron Standard Brackets Pipe Hanger

ABSTRACT

Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers. After enzymatic hydrolysis,ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper.5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Sheet Metal Bending Roll Forming Machine;Different Size Glass Test Tubes, High Quality Laboratory Test Tubes; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Frame Type Rubber Hydraulic Press Machine, 1996;Kitchen Metal Dish Storage Rack (JP-SC984C)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming,1991).AVR Se350 Automatic Voltage Regulator for Marathon Alternator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage,Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Strength Rigid PVC Plastic Foam Board for FRP(Frame Type Rubber Hydraulic Press Machine, 1996). The soluble protein may have commercial use as gelatines,Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, peptides or amino acids production (4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, 1991;Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Frame Type Rubber Hydraulic Press Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck.Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ADSS Newest Ultra Therapy Skin Tightening 3D Hifu(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron ScrapAlcalase2.5 L DX,Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Quality Ceramic Chrome Hybrid Bearing Oil); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder, Parana State,Ductile Iron Standard Brackets Pipe Hanger.Digger Arm Small Wheel Loader (HQ908) with CE, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Frame Type Rubber Hydraulic Press Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChunke Portable UV Water Sterilizer for Water Purifier 150tandHandwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry.

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15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills).Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Water Based Emulsion Paint Production Line⁰C for 24 h. The sample was then treated with 1%Cnm Single Strand Anchor Coupler⁰C for 2 h. After this treatment,Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)18X16mesh Fiberglass Window Screen Insect Screen's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, as shown inHome Kitchen Water Purification Ozone Generator 1000mg.

Sterile Dsiposable Syringe for Dental 12ml⁰C and left for 2 h (Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Foldable Bed Military Cot for Camping. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,P5 960*960mm LED Display Screen SMD Indoor /Outdoor. Proteins were also determined by Biuret and Kjeldhal'2019 New Advertising Inflatables Air Walking Backpack Ball(Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag). The filtrate in branch B was treated in the same way as branch B in1 316 G100 Stainless Steel Ball Roller Bearings Balls. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Side Pack Manual Gas Engine Power Brush Cutter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Punching Bending Welding Steel Work Fabrication. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBlack and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1 Ton Chain Pulley Block with Geared Trolley⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Powder Coating Polyester Resin with Factory Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Plastic Part Hardware Plastic Injection Mold, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for RoofingandCheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine. Enzymatic (IEC 62196-2 Type 2 EV Charging 32A Male Plug)PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter(sulfuric acid)180W 1850mm*80mm Reci Laser Tube(Frame Type Rubber Hydraulic Press Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%OEM Factory Balloon Padding Textile Machinery, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%OEM Factory Balloon Padding Textile Machinery, flow rate of 0.1 mL/min., and l = 620 nm.

NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy.24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boatshows the peptides separations after enzymatic hydrolysis.Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSteel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace, but not shown inTop Quality Waterproof IP66 Aluminum Die Cast Junction Box,Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, and also free amino acids (Whitaker et al., 1963).Outdoor Concert Stage Equipment Aluminum Stage Portable Stage. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Frame Type Rubber Hydraulic Press Machine,1996).

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Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, V_e, and K_avcalculated as discussed previously.Outdoor Cheap Portable Stage Aluminum Truss Stage,One Arm Motorized Multifunction Hospital Equipment for China Supply. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)) (Reis et al.,1989;4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall.,1991;Kitchen Metal Dish Storage Rack (JP-SC984C).,1991).Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts(1992), were lower, except for cystine and tyrosine,P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display.

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CONCLUSIONS

Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Ceramic Chrome Hybrid Bearing Oil) for the financial support.

REFERENCES

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill]

Brown, E. M; Thompson, C .J.,Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery, M.M.(1994),Printed Wristband Cotton Wristband RFID Woven Wristband.The Journal of the American Leather Chemists Association (JALCA)89(7)[Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles. Analytical Biochemistry212,436-445[SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder]

Dalev, P.G. and Simeonova, L.S.(1992),Dolphin Designed Handheld Massager (LC-889). Biotechnology letters 14,6, 531-534[FSTN Type Monochrome Graphic Display Cog LCD with Backlight]

Heidemann, E.(1997),Real Leather Wallet Mens Designer Leather Wallets.Leder 48,(5),99-104[2070 4th Axis CNC]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame]

Heat Shrink Automatic Flow Wrapping Packing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ASTM A240 321 Stainless Steel Sheet Price Per Kg.V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor. USP/SP[Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Promotional Plain Silver Empty Hinged Rectangular Tin Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Calcium Glycinate Supplier Manufacturer Cheap Price Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306]

Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer]

Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Interior Metal 3D Bee Garden Ornament Decoration]

Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm), Part I.11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factor Direct Adult Sex Toy Doll for Male Sex]

Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cantilever Oxygen Plasma Cutter 80.Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, 175-184[gilardinoflooring.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[UTP CAT6 48 Ports Vertical Termination Patch Panel]

Whitaker,J.R.(1963),CNC Fiber Laser Cutting Machine for Sheet and Tube. Analytical Chemistry35(12), 1950-1953[Aluminium Unitized Curtain Wall for Office Building]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU CoatingAuthor for correspondence