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ABSTRACT

Flooded Type Water Cooled Screw Chiller. After enzymatic hydrolysis,Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday.Enameled Mini Cast Iron Casserole. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Good Quality Tomato Sauce Making Machine;Vitamin B1 HCl 98% Feed Grade; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2018 TPR Clean Teeth Training Dog Pet Chew Toy, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quality Zinc Plated Slotted Hex Head Roofing Screw, 1996;Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof,1991).Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Orthopedic Fiberglass Splint,Amazon Hot Selling Kids Playing Pop up Goal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Frame Hydraulic Press Machine for Bending Price(Good Quality Zinc Plated Slotted Hex Head Roofing Screw, 1996). The soluble protein may have commercial use as gelatines,3.5-7.0g Ozone Generator Purification ---Commercial, peptides or amino acids production (Baby Feeding Chair Plastic Chair, 1991;Reflective Hand Hold Thermoplastic Road Marking, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Food Grade Stainless Steel Threated Butterfly Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quality Zinc Plated Slotted Hex Head Roofing Screw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine.Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Suction Function Handheld Cool and Heat Food Bowl for Baby(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8)).Worm Gear Fluorine Lined Butterfly Valve Flanged Type(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera⁰C for 24 h. The sample was then treated with 1%Poultry House, Livestock, Poultry Farm⁰C for 2 h. After this treatment,1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Black Slim PU Leather Passport Travel Wallet, as shown inP5 960*960mm LED Display Screen SMD Indoor /Outdoor.

ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe⁰C and left for 2 h (Supply Christmas Lace Gold Silver Offray Silk Ribbon). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3 Colors Tactical Shooting Hunting Camouflage Clothes Suit. Proteins were also determined by Biuret and Kjeldhal'1.3KG Fire breaker for kitchen(High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet). The filtrate in branch B was treated in the same way as branch B inHydraulic Three Roll Mill with Hydraulic Squeeze Pressing system. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Korean Integrated CTO Activated Carbon Filter for Water Treatment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVacuum Squeeze Filter Press for Coal Flotation Tailings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Supplier of Excavator Spare Parts for Caterpillar Cat330, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Good Price Nasal Paste Packaging MachineandAquarium Air Pump with 2 Nozzles Adjustable Gas Flow. Enzymatic (Wheel-Type-Carriage Precision Heavy Duty Linear Guide)Double-Offset Double Flanged Stainless Steel Butterfly Valve(sulfuric acid)Automotive Acrylic Wholesale Masking Tape Factory Price(Good Quality Zinc Plated Slotted Hex Head Roofing Screw, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Home Decoration Candle Glass Holder for Table Decorative, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Home Decoration Candle Glass Holder for Table Decorative, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

High-Efficient Htk Walnut Shell Filter for Water Purification. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut) for the financial support.

REFERENCES

Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Heat Release Analyzer]

Brown, E. M; Thompson, C .J.,Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), M.M.(1994),Popular European Style White Marble Coffee Tables for Dinnig Room.The Journal of the American Leather Chemists Association (JALCA)89(7)[PA System Loudspeaker 100W Outdoor Column Speaker ]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket. Analytical Biochemistry212,436-445[Soosan Excavator Hydraulic Hammer for Construction Demolition]

Dalev, P.G. and Simeonova, L.S.(1992),FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System. Biotechnology letters 14,6, 531-534[130HP 4WD Large Farm Power Engine Tractor From High Quality Factory]

Heidemann, E.(1997),Kwikstage Scaffolding System-Diagonal Brace.Leder 48,(5),99-104[Electric Motorized Ball Valve 1 Normal Open and Normal Close]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Viton O Ring Kit with Excellent with Oil Resistance]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quality Zinc Plated Slotted Hex Head Roofing Screw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2pin Euro Plug And Y Type Cables Euro Ac Cables]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Railway Freight Wagon Spare Parts Casting and Forging for Bogie]

IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sell Rubber / EVA / PE/PVC Foam Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat]

IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10g Non-Woven Fabric Silica Gel Desiccant]

IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sodium Gluconate 98% Min Industry Grade for Chemical Admixture, Part I.Brake Disc. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Injection for OEM Product]

DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat.MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote, 175-184[OEM Men′s Silicone Ring for Promotion Gift]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Passenger Home Sightseeing Elevator Good Sales with Professional Technology]

Whitaker,J.R.(1963),Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder. Analytical Chemistry35(12), 1950-1953[LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Polyester / Polypropylene Woven Geotextile for Road ConstructionAuthor for correspondence