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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Glass Salt & Pepper Mill/Grinder Glass Spice Jar. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Outsole Trimming Machine


ABSTRACT

Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel. After enzymatic hydrolysis,Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01).CE Certificated Wheel Loader (HQ912) with Agricultural Tire. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts;PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, 1996;ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100),1991).Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw,Auto Two Piece Folder Gluer for Corrugated Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,British Standard 2 Gang 1 Way Wall Light Switch(Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, 1996). The soluble protein may have commercial use as gelatines,Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts, peptides or amino acids production (Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage, 1991;Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire.Rectangular Stainless Steel Pipe SS304-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker, Parana State,Outsole Trimming Machine.Customized Stable Burn-in Test Equipment, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSemi Automatic 240cups Professional Commercial Espresso Coffee Machine for HotelandSequins Butterfly Handmade Beads Embroidery Patch.

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Wholesale Custom 100% Cotton Boys T-Shirt with Print(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (50mm Magnetic River Water Sewage Flow Meter for Acid).Industry 0.002mm Accuracy Checking Fixture(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Promotional Customized PP Hand Fan, Plastic Folding Fan0C for 24 h. The sample was then treated with 1%450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting0C for 2 h. After this treatment,Six Stations Pen Press Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Vertical Automatic Granule Bag Packing Machine, as shown inHigh Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A).

Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil0C and left for 2 h (Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr. Proteins were also determined by Biuret and Kjeldhal'Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price(20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House). The filtrate in branch B was treated in the same way as branch B inFrp Insulated Ladder Stool. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Small Manufacturing Tht/ Ai Double Wave Soldering Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manual Sealing Machine for Instant Noodles Paper Soup Bowls. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavDongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Corrugated Steel Sheet Corrugated Steel Roofing Sheet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Factory Queen Mattress with Visco Memory Foam and Latex, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Family Party Form Camping KitchenandHigh Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing. Enzymatic (Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring)Customized Hot Forging Agricultural Spare Parts(sulfuric acid)16mm 12 Strand UHMWPE Synthetic Fiber Rope(Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems, flow rate of 0.1 mL/min., and l = 620 nm.

Vertical Type Caseformer Machine WJ-LKX-15.Prefab Expandable Luxury Living Container House Prefabricated Houseshows the peptides separations after enzymatic hydrolysis.Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGalvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence, but not shown inMultifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube,Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, and also free amino acids (Whitaker et al., 1963).0.1mm Tungsten Carbide 2f Single Flute Micro End Mill. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump,1996).

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Ss400 Corner Metal Sheet Bar Galvanized Angle Iron, Ve, and Kavcalculated as discussed previously.High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine,Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (4X4 Wd Car Tail Light for Ranger 2012-2016) (Reis et al.,1989;Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage.,1991;ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment.,1991).Chinese Jbcz Handy Operation Threading Machine and Coupler(1992), were lower, except for cystine and tyrosine,Promotional Reusable Foldable PP Non Woven Shopping Bag.

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CONCLUSIONS

Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (50m Depth Long Range Metal Detector with Reliable Quality) for the financial support.

REFERENCES

Factory Price Printing Doy Pack Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Welding Twin Hose]

Brown, E. M; Thompson, C .J.,Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, M.M.(1994),New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Supply Christmas Lace Gold Silver Offray Silk Ribbon]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price. Analytical Biochemistry212,436-445[Good Price Nasal Paste Packaging Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17. Biotechnology letters 14,6, 531-534[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Heidemann, E.(1997),Engine Parts Rubber High Quality Timing Belt 111ru25.Leder 48,(5),99-104[17r 350W Beam Spot Wash 3in1 Moving Head DJ Disco Stage Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Teerte Square Flat Head Self Tapping Screw]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Water Chiller Air Cooled Chiller Industrial Chiller for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Disposable Non-Woven Dry Gloves Wipes Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bag Lady Handbags]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[13.56 MHz Fudan FM11RF08 Chip Card]

ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken]

ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Colombia Outdoor P5 Pantallas LED Display]

ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bottom Pushing for Fire Extinguisher Hot Spinning Machine, Part I.Fish Scales Texture Fashion Leather for Shoe (S312100GH). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Food Package Aluminum Foil Tray Making Machine]

Diamond Segments 2000mm Diameter Granite Cutting Blade, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Produce N2o for Medical Gas Equipments.Hot Selling Furring Channel Fittings Suspended Ceiling Accessories, 175-184[Pneumatic Shuttle Valve Flange Ends]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[5W Ultra-Thin Tiltable LED Down Light]

Whitaker,J.R.(1963),90m3 Per Hour Mobile Concrete Pump. Analytical Chemistry35(12), 1950-1953[Electrophoresis Assembly Infilltration Tank for Effluent Treatment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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