ARTICLES

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ABSTRACT

Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers. After enzymatic hydrolysis,50m Depth Long Range Metal Detector with Reliable Quality.CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Custom 100% Cotton Boys T-Shirt with Print, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industry 0.002mm Accuracy Checking Fixture, 1996;Promotional Customized PP Hand Fan, Plastic Folding Fan., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting,1991).Six Stations Pen Press Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine,Vertical Automatic Granule Bag Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil(Industry 0.002mm Accuracy Checking Fixture, 1996). The soluble protein may have commercial use as gelatines,160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures, peptides or amino acids production (4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, 1991;Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Small Manufacturing Tht/ Ai Double Wave Soldering Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Manual Sealing Machine for Instant Noodles Paper Soup Bowls.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industry 0.002mm Accuracy Checking Fixture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading.190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Corrugated Steel Sheet Corrugated Steel Roofing Sheet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Vertical Type Caseformer Machine WJ-LKX-15(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Size Christmas Gift Personalized Watch Boxes for Men).Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould⁰C for 24 h. The sample was then treated with 1%0.1mm Tungsten Carbide 2f Single Flute Micro End Mill⁰C for 2 h. After this treatment,Agriculture Large Span Commerical Steel Frame Greenhouse. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, as shown inGood Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.

Ss400 Corner Metal Sheet Bar Galvanized Angle Iron⁰C and left for 2 h (Automotive Perforated Metal Stamping Part for Furniture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer. Proteins were also determined by Biuret and Kjeldhal'Chinese Jbcz Handy Operation Threading Machine and Coupler(Fancy Veneer Plywood. Natural Veneer Plywood for Furniture). The filtrate in branch B was treated in the same way as branch B inMarine And Coastal Structures. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Promotional Reusable Foldable PP Non Woven Shopping Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Price Printing Doy Pack Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring) for the financial support.

REFERENCES

for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminium Oxide Material Al2O3 Granules for Optical Coating]

Brown, E. M; Thompson, C .J.,100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree, M.M.(1994),School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056).The Journal of the American Leather Chemists Association (JALCA)89(7)[Glass Door Passenger Sightseeing Elevator for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle. Analytical Biochemistry212,436-445[High Quality Lead Ingot 99.994 Reasonable Price]

Dalev, P.G. and Simeonova, L.S.(1992),Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation. Biotechnology letters 14,6, 531-534[ Half Nut]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vacuum Squeeze Filter Press for Coal Flotation Tailings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cracker Multi Rows Trayless Packaging Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[All in One Motion Sensor Integrated Solar Street Light LED]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels]

Crop Fertilizer Agriculture Nh4cl Ammonium Chloride, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale High Pressure 80L Nitrogen Gas Cylinder-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System]

Crop Fertilizer Agriculture Nh4cl Ammonium Chloride,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bottom Pushing for Fire Extinguisher Hot Spinning Machine]

Crop Fertilizer Agriculture Nh4cl Ammonium Chloride, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:10mm Red Green Yellow Warning China Supplier Signal Lamp, Part I.Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[80GSM C2s Coated Glossy/Matt Art Paper for Printing]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Real Leather Wallet Mens Designer Leather Wallets]

Whitaker,J.R.(1963),Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll. Analytical Chemistry35(12), 1950-1953[Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Cross Recesed Flat Head Self Tapping ScrewAuthor for correspondence