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Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-LED Screen Display Wireless Charging 10000mAh Power Bank. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,25kg 50kg Plastic PP Woven Feed Bags


360 Degree Rotation Medical Seat Cushion. After enzymatic hydrolysis,Custom LCD Screen Video Box for Package.Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning;2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Alu 2319 Slimline 12mm Wide Recessed Profile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, 1996;Custom Size Christmas Gift Personalized Watch Boxes for Men., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope,1991).8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality,New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)(High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Cross Recesed Flat Head Self Tapping Screw, peptides or amino acids production (Our Perforated Aluminum & Solid Panel for New Office Wall Cladding, 1991;2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Kids Trucker Hat, Kids Mesh Cap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wd190V Handheld Full Body Scanner Hand Held Metal Detector.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Hydraulic Orbital Valve Power Steering.W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:AISI 410 Stainless Steel Galvanized Wire for Making Scourer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

ISO 3821 3/16′′ Twin Line Welding Hose for USA Market, Parana State,25kg 50kg Plastic PP Woven Feed Bags.Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSmall Leather Shoes Craft Laser Cutting Machineand1 Inch NPT Screwed Globe Valve.

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Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal).Factory Stock Spring Steel U Type Clip Nut(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ASME VIII Pressure Vessel for Curing Carbon Fiber Parts0C for 24 h. The sample was then treated with 1%Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck0C for 2 h. After this treatment,Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Portable Electric Concrete Vibrator with 18000 High Rotation Speed's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster, as shown inCNC Machining 6601 Aluminum Parts with Anodized Automoblile.

Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb41220C and left for 2 h (Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing. Proteins were also determined by Biuret and Kjeldhal'UCP Color Army Camouflage Uniform Army Military Uniform Suit(Food Grade Silicone Baby Pacifier for Newborns). The filtrate in branch B was treated in the same way as branch B inAluminum Foil Bake Pan. The final soluble protein shown were used for peptides separations using gel permeation chromatography,0.5 Gallons Cool Mist Ultrasonic Humidifier.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavSanjing PVC Lining Layflat Fire Hose(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic CNC Glass Cutting Machine Glass Cutting Table, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)andAutomatic Moder Double Seal Nipple Drinking System for Broiler Chicken. Enzymatic (Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price)Pet PP Rope and Broom Wire Drawing Machine(sulfuric acid)14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup(High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PTFE Seat Wafer Butterfly Valve Pn16, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PTFE Seat Wafer Butterfly Valve Pn16, flow rate of 0.1 mL/min., and l = 620 nm.

Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part.Quality Shaper Machine Bc6085 Shapping Machineshows the peptides separations after enzymatic hydrolysis.China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEbay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, but not shown in16mm 12 Strand UHMWPE Synthetic Fiber Rope,Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, and also free amino acids (Whitaker et al., 1963).Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo,1996).

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Blue White Yellow Red Black Nylon Mosquito  Window NettingandConcrete Aluminum Formwork System Aluminium Formworkshow the gel chromatographic separation of soluble protein with Sephadex G-Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt.Plain Makeup Bag Canvas Cosmetic Bag, and another peak at 45 mL not shown inWorm Gear Fluorine Lined Butterfly Valve Flanged Type, for peptides lower than 3000 Da.14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow(Condell et al.,1993) .

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1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, Ve, and Kavcalculated as discussed previously.High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple,Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W) (Reis et al.,1989;Our Perforated Aluminum & Solid Panel for New Office Wall Cladding.,1991;Custom Size Christmas Gift Personalized Watch Boxes for Men.,1991).Factory Wholesale Qualited Polyester Padding Men Jacket(1992), were lower, except for cystine and tyrosine,5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam.

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Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile) for the financial support.


Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 Summer Fresh Landscaping Turf Lawn]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-River Water Cleaning Sludge Sediment Machine Lamella Clarifier. Analytical Biochemistry212,436-445[Wholesale Custom 100% Cotton Boys T-Shirt with Print]

Dalev, P.G. and Simeonova, L.S.(1992),Polyester Coated Stainless Steel Ladder Type Metal Ties. Biotechnology letters 14,6, 531-534[T6s 86 PCD Core Bit for Geotechnical Drilling]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flooded Type Water Cooled Screw Chiller]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Halal Empty Gelatin Capsule for Medicine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3D Large Format Sublimation Heat Press Machine (ST-3042)]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Size Christmas Gift Personalized Watch Boxes for Men]

Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gym Fitness Exercise Sporting Body Building Bicep Press-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines]

Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automotive Perforated Metal Stamping Part for Furniture]

Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck, Part I.Metal Steel Frame Membrane Structure Car Parking Shed Cover Design. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fancy Veneer Plywood. Natural Veneer Plywood for Furniture]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Design and Nice Price All in One Solar Street Light]

Whitaker,J.R.(1963),Polyester Dust Sport Gym Fabric Shoes Drawstring Bag. Analytical Chemistry35(12), 1950-1953[Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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