ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Linen Quiltcover Set with Quilting. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life

ABSTRACT

Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace. After enzymatic hydrolysis,Teerte Square Flat Head Self Tapping Screw.Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Selling Military Large Event Marquee Tent Sale, 1996;High-Quality Pre-Seasoned Cast Iron Wok., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle,1991).Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Marine High Visibility Solas Reflective Tape,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)(Hot Selling Military Large Event Marquee Tent Sale, 1996). The soluble protein may have commercial use as gelatines,Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, peptides or amino acids production (Magic Tape Hook and Loop Fasteners, 1991;Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Starch Doy Bag Packaging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Selling Military Large Event Marquee Tent Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A).Fashion Soft PVC Rubber Keychains for Promation Gift-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Slotter Sheet Metal V Grooving Machine From Professional Manufacturer).Custom Grey Full-Zip Cotton Mens Hoodies(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook⁰C for 24 h. The sample was then treated with 1%7inch Industrial Tablet Computer Naked Resistance Touch Screen⁰C for 2 h. After this treatment,Glass Door Passenger Sightseeing Elevator for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, as shown inElectronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Premium Grade Rubber Cloth Duct Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSud1000h Poly Welder Butt Fusion Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare PartsandOEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic. Enzymatic (PP Woven Bag, Rice, Beans, Plastic Bag)China Professional Mold Factory Plastic Injection Mould Maker(sulfuric acid)Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated(Hot Selling Military Large Event Marquee Tent Sale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments) for the financial support.

REFERENCES

High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dual Isolator Manufacturers]

Brown, E. M; Thompson, C .J.,Pet Laminate Aluminium Foil/Pet+Alu+PE, M.M.(1994),6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[ CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Design Multi-Color Silicone Coin Purse for Lady. Analytical Biochemistry212,436-445[Taiding Custom Tank Sheet Metal Fabrication]

Dalev, P.G. and Simeonova, L.S.(1992),Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065. Biotechnology letters 14,6, 531-534[BS 5163 Ductile Iron Rising Stem Gate Valve]

Heidemann, E.(1997),Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen.Leder 48,(5),99-104[Factory Best Sodium Carboxymethyl Cellulose CMC for Oil Drilling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair]

Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dark Green Plastic PVC Film for Christmas Tree Leaves.Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor. USP/SP[Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4-14 Manual Staple Gun with Staples for Hand Tools]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Direct Sales Energy Efficient DC Solar Centrifugal Water Pump (S122T-30)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile]

1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Water Treatment Anthracite-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant]

1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wd190V Handheld Full Body Scanner Hand Held Metal Detector]

1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heat Treated Welding Protection Glassfiber Cloth Ht800, Part I.Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Single Board 3.7V~24VDC Microwave Detector PCB]

Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heat Pump Non-Programmable HVAC Room Thermostat.Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, 175-184[Children Action Camera]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic]

Whitaker,J.R.(1963),Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable. Analytical Chemistry35(12), 1950-1953[Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Precision Plastic Mold Manufacturer in ChinaAuthor for correspondence