River Water Cleaning Sludge Sediment Machine Lamella Clarifier

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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace. After enzymatic hydrolysis,Teerte Square Flat Head Self Tapping Screw.Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Bto 22 Electric Galvanized Razor Concertina Wire for Military;Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Selling Military Large Event Marquee Tent Sale, 1996;High-Quality Pre-Seasoned Cast Iron Wok., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle,1991).Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Marine High Visibility Solas Reflective Tape,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)(Hot Selling Military Large Event Marquee Tent Sale, 1996). The soluble protein may have commercial use as gelatines,Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, peptides or amino acids production (Magic Tape Hook and Loop Fasteners, 1991;Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Starch Doy Bag Packaging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Selling Military Large Event Marquee Tent Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A).Fashion Soft PVC Rubber Keychains for Promation Gift-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, Parana State,2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life.304 &314 Stainless Steel Seamless Bending Tube, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Hot Selling Military Large Event Marquee Tent Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDolphin Designed Handheld Massager (LC-889)andWholesale Prices of 20W 40W 60W Solar Street Lights.

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Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Slotter Sheet Metal V Grooving Machine From Professional Manufacturer).Custom Grey Full-Zip Cotton Mens Hoodies(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook0C for 24 h. The sample was then treated with 1%7inch Industrial Tablet Computer Naked Resistance Touch Screen0C for 2 h. After this treatment,Glass Door Passenger Sightseeing Elevator for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, as shown inElectronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve.

Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple0C and left for 2 h (New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Fcy30 Transport Mini Tipping Dumper. Proteins were also determined by Biuret and Kjeldhal'Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park(FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System). The filtrate in branch B was treated in the same way as branch B inKallaite. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Premium Grade Rubber Cloth Duct Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavSud1000h Poly Welder Butt Fusion Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare PartsandOEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic. Enzymatic (PP Woven Bag, Rice, Beans, Plastic Bag)China Professional Mold Factory Plastic Injection Mould Maker(sulfuric acid)Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated(Hot Selling Military Large Event Marquee Tent Sale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, flow rate of 0.1 mL/min., and l = 620 nm.

Industrial Carbon Steel Wire Hose Hydraulic Pipe.12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Ropeshows the peptides separations after enzymatic hydrolysis.24k Gold Whitening Anti-Spot Mask(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in5000-5900MHz 4G Built-in Automotive Antenna, but not shown inUCP Color Army Camouflage Uniform Army Military Uniform Suit,Clay Tiles Making Machine with Latest Technology, and also free amino acids (Whitaker et al., 1963).DIN Standard Gratings/Gavlanized Steel Grating. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Selling Military Large Event Marquee Tent Sale,1996).

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Corrugated Steel Sheet Corrugated Steel Roofing Sheetand Caulk Strip Trim Tape (38mm)show the gel chromatographic separation of soluble protein with Sephadex G-50mm Magnetic River Water Sewage Flow Meter for Acid.PE Biodegradable Sheet Product Line/Single Screw Extruder, and another peak at 45 mL not shown inLong Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress, for peptides lower than 3000 Da.27L/Min Water Exchanger Heat Pump Mold Temperature Machine(Condell et al.,1993) .

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Felt Belt for Textile Compactor, Ve, and Kavcalculated as discussed previously.Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt,Metal Soft Enamel Souvenir Metal Medal Sport Medallions. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Heavy Duty Marquee Advertising Pop up Tent) (Reis et al.,1989;Magic Tape Hook and Loop Fasteners.,1991;High-Quality Pre-Seasoned Cast Iron Wok.,1991).20L Water Tank Lid Mold 3D Printing Samples Available(1992), were lower, except for cystine and tyrosine,Wholesale High Quality Metal Car Mount Holder Phone Holder.

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Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments) for the financial support.


High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dual Isolator Manufacturers]

Brown, E. M; Thompson, C .J.,Pet Laminate Aluminium Foil/Pet+Alu+PE, M.M.(1994),6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[ CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Design Multi-Color Silicone Coin Purse for Lady. Analytical Biochemistry212,436-445[Taiding Custom Tank Sheet Metal Fabrication]

Dalev, P.G. and Simeonova, L.S.(1992),Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065. Biotechnology letters 14,6, 531-534[BS 5163 Ductile Iron Rising Stem Gate Valve]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4-14 Manual Staple Gun with Staples for Hand Tools]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Direct Sales Energy Efficient DC Solar Centrifugal Water Pump (S122T-30)]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile]

1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Water Treatment Anthracite-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant]

1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wd190V Handheld Full Body Scanner Hand Held Metal Detector]

1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heat Treated Welding Protection Glassfiber Cloth Ht800, Part I.Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Single Board 3.7V~24VDC Microwave Detector PCB]

Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heat Pump Non-Programmable HVAC Room Thermostat.Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, 175-184[Children Action Camera]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic]

Whitaker,J.R.(1963),Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable. Analytical Chemistry35(12), 1950-1953[Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Precision Plastic Mold Manufacturer in ChinaAuthor for correspondence

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