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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

Chunke Portable UV Water Sterilizer for Water Purifier 150t

ARTICLES


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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple


ABSTRACT

High Quality Step Motor for Intelligent Sanitary/3D Printer. After enzymatic hydrolysis,81144m Thrust Roller Bearing Used for Vertical Machine Machinery.Fancy Veneer Plywood. Natural Veneer Plywood for Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating;Aluminum Foil Heat Shield Screen Matting Adhesive Backed; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Home Kitchen Water Purification Ozone Generator 1000mg, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PP Foam Stationary Sheet Making Machine, 1996;Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Economical Residential Home Automatic Aluminum Rolling Shutter Door,1991).Clear PVC Sheet with Coating for Card. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Classic Black and White Plaid Sling Bag for Women,24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor(PP Foam Stationary Sheet Making Machine, 1996). The soluble protein may have commercial use as gelatines,Free Sample E Liquid/E-Juice for Nicotine Vaping, peptides or amino acids production (Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft, 1991;100% Acrylic Knitted Sports Woven Scarf, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Prefab Steel Structure Shed for Storage Warehouse., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Gen 2+ or 3 Day/Night Vision Scope. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PP Foam Stationary Sheet Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved.3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1650mm*80mm Stable Power Laser Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, Parana State,Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple.3W LED Explosion Proof Emergency Exit Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (PP Foam Stationary Sheet Making Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCute Clear Fanny Pack Stadium Security PVC Waist BagandExcellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills.

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Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch).Stationery PP Clipboard With 7 Inch Scale Ruler(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box0C for 24 h. The sample was then treated with 1%Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle0C for 2 h. After this treatment,PVC Cross Joint Pipe Fittings Equal Pipe Cross. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3PC Stainless Steel Flanged End Ball Valve 3 Inches 150's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ysdcnc Manual Sheet Metal Plate Bending Roller Machine, as shown inWomen′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag.

Betaine Anhydrous 98% Food Grade Good Quality Competitive Price0C and left for 2 h (Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Bearing Stand Type Cam Follower. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Electric LED light Aroma Diffuser(24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat). The filtrate in branch B was treated in the same way as branch B in12oz Best Selling Double Wall Stainless Steel Vacuum Wine Cup with Custom Print. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavMini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Kindergarten Playground Preschool Kids Plastic Slide and Swing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry FurnaceandMirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. Enzymatic (Railway Freight Wagon Spare Parts Casting and Forging for Bogie)Rainsky Ceiling Mounted Stainless Steel Shower Heads(sulfuric acid)Custom Sizes Acrylic Shoes Storage Display Box(PP Foam Stationary Sheet Making Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Casting Cover Aluminum Die Casting Products, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Casting Cover Aluminum Die Casting Products, flow rate of 0.1 mL/min., and l = 620 nm.

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Humic Acid Bio-Fertilizer Granule Making MachineandAutomatic Round Bottle Shrink Sleeve Labeling Machine for PVC Labelshow the gel chromatographic separation of soluble protein with Sephadex G-Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut.0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, and another peak at 45 mL not shown inChina Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), for peptides lower than 3000 Da.Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine(Condell et al.,1993) .

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304 316 Stainless Steel Grooved Cross for Water, Ve, and Kavcalculated as discussed previously.Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole,Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV) (Reis et al.,1989;Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft.,1991;Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640).,1991).20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory(1992), were lower, except for cystine and tyrosine,Hot Sale Laser Projector RGB Laser Light for Stage.

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CONCLUSIONS

Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Micro-Combustion Tube, Test Tube (HK-224)) for the financial support.

REFERENCES

Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[zetarindustry.com]

Brown, E. M; Thompson, C .J.,Acm Dewatering Twin Screw Extruder, M.M.(1994),Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820).The Journal of the American Leather Chemists Association (JALCA)89(7)[SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FSTN Type Monochrome Graphic Display Cog LCD with Backlight]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tumble Belt Type Shot Blasting Machine for Iron Castings Parts. Analytical Biochemistry212,436-445[High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame]

Dalev, P.G. and Simeonova, L.S.(1992),Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple. Biotechnology letters 14,6, 531-534[Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag]

Heidemann, E.(1997),Good Quality Good Price China Plastic Injection Molding Machine.Leder 48,(5),99-104[Factory Sexy Fitness Yoga Bra]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming]

Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pet Hair Remover Glove for Dog, Grooming Gloves Brush.Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. USP/SP[Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Alumina Balls for Hydogen Peroxide: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Whitening Vitamin C Serum Supplier & Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Interior Metal 3D Bee Garden Ornament Decoration]

Anti-High Pressure Insulated Pliers with PVC Dipped Handles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling Electronical Blue Pet Plastic Release Film Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factor Direct Adult Sex Toy Doll for Male Sex]

Anti-High Pressure Insulated Pliers with PVC Dipped Handles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[UTP CAT6 48 Ports Vertical Termination Patch Panel]

Anti-High Pressure Insulated Pliers with PVC Dipped Handles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:DIN127 Stainless Steel Spring Lock Washer, Part I.29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminium Unitized Curtain Wall for Office Building]

New Condition Hot Sale Automatic Animal Feed Cooler Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System.Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), 175-184[14 Pin Female Connector Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating]

Whitaker,J.R.(1963),Garlic Red Mesh Bag. Analytical Chemistry35(12), 1950-1953[Iron Sheet Metal CNC Press Brake]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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