ARTICLES

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ABSTRACT

Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. After enzymatic hydrolysis,Printed Wristband Cotton Wristband RFID Woven Wristband.High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Dolphin Designed Handheld Massager (LC-889);Real Leather Wallet Mens Designer Leather Wallets; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ASTM A240 321 Stainless Steel Sheet Price Per Kg, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, 1996;Promotional Plain Silver Empty Hinged Rectangular Tin Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling,1991).5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm),11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)(V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, 1996). The soluble protein may have commercial use as gelatines,Cantilever Oxygen Plasma Cutter 80, peptides or amino acids production (Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, 1991;CNC Fiber Laser Cutting Machine for Sheet and Tube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval.High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DIN929 Low-Cost Supply Hexagon Weld Nut).Platform Trolley with 150kg Capacity, 4 Castor Wheel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine⁰C for 24 h. The sample was then treated with 1%LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V⁰C for 2 h. After this treatment,All Size Colored Tapping Hex Head Self Drill Screw Wood Screw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pre-Engineered Cage Broiler Poultry Farm Building's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Alumina Ball with High Quality, as shown inHome Decoration Candle Glass Holder for Table Decorative.

PVC Textile Fabric for Outdoor Sports Waterproof Backpack⁰C and left for 2 h (Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.White 4*8FT 18mm PVC Foam Board for Cabinets Use. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating. Proteins were also determined by Biuret and Kjeldhal'Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants(CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine). The filtrate in branch B was treated in the same way as branch B inLed Rigid Strip. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Powerful Anti-Stress, Strong Flexibility Spoke Wire.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Modern American Style Beech Solid Wood Kitchen Cabinet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHero Brand Polypropylene Bags Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Surprise! Range Hood Free! ! ! Juice Cart⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chicken Layer Cage Poultry Cage Chicken Cages, pH 3.25., mobile phase B: buffer solution of 0.01 mol/316L Cold Rolled Stainless Steel Coil 316L Inox, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

12ml Round Shape Empty Clear Nail Polish Glass Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Aluminum Sheet Press Brake) for the financial support.

REFERENCES

M4X10 Round Head Core Pulling Aluminum Nail, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[chinacarmates.com]

Brown, E. M; Thompson, C .J.,Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, M.M.(1994),Skytone 4 Channels 3500W Each DJ Power Amplifier.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price. Analytical Biochemistry212,436-445[Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring]

Dalev, P.G. and Simeonova, L.S.(1992),PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels. Biotechnology letters 14,6, 531-534[Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting]

Heidemann, E.(1997),2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture.Leder 48,(5),99-104[Canvas Knife Roll Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fire Extinguisher UV Curing Machine Cylinder Drying Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Betaine 98% Betaina Feed Grade]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Digital Code Gun Lock]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Vertical Automatic Granule Bag Packing Machine]

Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flexible PVC Granules for Cable, Wire and Insulation-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)]

Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal]

Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security, Part I.Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa]

Brass Pump Spare Parts for Circulating Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes.Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi, 175-184[dvds - Dvd Management Rfid Sticker]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Damask Pillows Cover]

Whitaker,J.R.(1963),Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011. Analytical Chemistry35(12), 1950-1953[Hf Vacuum Dryer Machine with Advanced High Frequency Technology]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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