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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery. After enzymatic hydrolysis,3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting.Personality Cat Eye Sunglasses. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline;UV Coated PVC Panel for Interior Wall Board; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring., about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, 1996;Wholesale 30W LED Down Lights with Budget Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Embossed Textured Wallpaper for Solvent Printing,1991).Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4-14 Manual Staple Gun with Staples for Hand Tools,500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Smallest Portable Waste Transformer Oil Purifier Machine Zy-6(Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, 1996). The soluble protein may have commercial use as gelatines,Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, peptides or amino acids production (High Speed Automatic Packing Machine (GK-650 BA), 1991;5000-5900MHz 4G Built-in Automotive Antenna, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Glass Lined Reactor for Chemical Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Metal Stamping Stainless Steel Parts with CNC Machining.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Supermarket Plastic T-Wire Basket Swing up Price Label Holder.1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless PipeAlcalase2.5 L DX,Degradable PLA Plastic Bottle, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Wholesale China Merchandise 12V Horizontal Mini Camping Fridge); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet, Parana State,High Speed 3000W Fiber Laser Cutter.2D LED Rope Lighting Motif Lights for Christmas Decoration, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHeat Shrink Automatic Flow Wrapping Packing MachineandPolyester Dust Sport Gym Fabric Shoes Drawstring Bag.

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3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Nh4cl White Granular Agricultural Grade Ammonium Chloride).10ml Dropper Bottle for Skin Care Packing(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom High Precision Micro Machining Service Brass Micromachining Parts0C for 24 h. The sample was then treated with 1%7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag0C for 2 h. After this treatment,Replacement Cell Phone LCD for iPhone 6s Spare Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Explosion Proof Fog Cannon for Coal Plant, as shown inNew Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt.

Silicone Large Non-Stick BPA Free Baking Mat Sheet0C and left for 2 h (Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.19mic Black Cast PE Stretch Film for Pallet Wrapping. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens. Proteins were also determined by Biuret and Kjeldhal'Custom Design Plush Panda Animal Slippers Comfort Foam Support by(3PC Stainless Steel Flanged End Ball Valve 3 Inches 150). The filtrate in branch B was treated in the same way as branch B inNut Plug / Nut Shell Granules / Drilling Addtive / Coarse. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best 6-Axis Electric Screwdriver Machine Robot.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavGalvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multi-Function Small Tree Digging Shovel/Tree Machine0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic L Bar Sealer Shrink Tunnel Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Full Automatic Carbonated Water Filling Full Line, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machineand2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile. Enzymatic (Superior Watertightness JS Polymer Cement Waterproof Coating for Basement)PS 200 Upper Discharge Centrifuge(sulfuric acid)3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew(Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, flow rate of 0.1 mL/min., and l = 620 nm.

Electrosurgical Generator High Frequency Electricity Kinfe.Halal Chicken Slaughter Machine for Saleshows the peptides separations after enzymatic hydrolysis.Viton O Ring Kit with Excellent with Oil Resistance(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Wire and Insulation, but not shown in12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor,One Step HBV 5 in 1 Rapid Test, and also free amino acids (Whitaker et al., 1963).Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device,1996).

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Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckleand304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispensershow the gel chromatographic separation of soluble protein with Sephadex G-2019 Summer Fresh Landscaping Turf Lawn.Pipe Sheet Laser Cutter with Tube Cutting, and another peak at 45 mL not shown inParty Mask LED Fiber Dance Princess Feather Mask, for peptides lower than 3000 Da.Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan(Condell et al.,1993) .

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Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils, Ve, and Kavcalculated as discussed previously.Supply Christmas Lace Gold Silver Offray Silk Ribbon,Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ductile Iron Standard Brackets Pipe Hanger) (Reis et al.,1989;High Speed Automatic Packing Machine (GK-650 BA).,1991;Wholesale 30W LED Down Lights with Budget Price.,1991).Toast Stamp Loaf Bread Tin Pan (ZMA)(1992), were lower, except for cystine and tyrosine,Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light.

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ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale China Merchandise 12V Horizontal Mini Camping Fridge) for the financial support.


Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[]

Brown, E. M; Thompson, C .J.,Baking Soda 99.8% Min Sodium Bicarbonate, M.M.(1994),BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized.The Journal of the American Leather Chemists Association (JALCA)89(7)[UV Coated PVC Panel for Interior Wall Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine. Analytical Biochemistry212,436-445[Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire]

Dalev, P.G. and Simeonova, L.S.(1992),6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower. Biotechnology letters 14,6, 531-534[Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor]

Heidemann, E.(1997),Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder.Leder 48,(5),99-104[180 Degree Revolve Work Light/camping Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures]

Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),10g Non-Woven Fabric Silica Gel Desiccant.Soosan Excavator Hydraulic Hammer for Construction Demolition. USP/SP[Line Scan Uvss System Portable Under Vehicle Surveillance]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electric 100W Three Wheels Toy Car for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[43 Inch All in One Wall Mounted Advertising WiFi Computer Monitor Touch Screen]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life]

Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home]

Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication]

Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950, Part I.Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box]

600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Cacao Bean Grinder Cocoa Butter Making Machine.Nh4cl White Granular Agricultural Grade Ammonium Chloride, 175-184[Stainless Steel Philips Countersunk Head Self-Tapping Screw]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws]

Whitaker,J.R.(1963),Concrete Aluminum Formwork System Aluminium Formwork. Analytical Chemistry35(12), 1950-1953[Heat Treated Welding Protection Glassfiber Cloth Ht800]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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